Viscoelastic Behavior of Thermoresponsive Polymer Hydrogel with Organic Adsorbate Observed Using Quartz Crystal Microbalance

2007 ◽  
Vol 36 (10) ◽  
pp. 1204-1205 ◽  
Author(s):  
Yuri Nakano ◽  
Itaru Sato ◽  
Yoshimi Seida ◽  
Yoshio Nakano
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Viscoelastic Behavior ◽  
Thermoresponsive Polymer ◽  
Polymer Hydrogel

Cellular Adhesion and Spreading of Endothelial Cells Monitored in Real Time Using the Quartz Crystal Microbalance

1998 ◽  
Vol 550 ◽  
Author(s):  
Tiean Zhou ◽  
Susan J. Braunhut ◽  
Diane Medeiros ◽  
Kenneth A. Marx
Keyword(s):  
Endothelial Cells ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Cell Mass ◽  
Viscoelastic Behavior ◽  
Cellular Adhesion ◽  
Frequency Change ◽  
Resistance Change ◽  
Continuous Increase ◽  
Mock Cell

AbstractWe have applied the Quartz Crystal Microbalance (QCM) technique to continuously record the processes of endothelial cell (EC) adhesion, spreading and cellular mass distribution changes during initial cell to surface contact and homeostatic attachment. ECs (50,000) were layered onto a set volume of media in the QCM device and simultaneously in a mock cell used for photomicroscopy. As cells were observed in the mock cell device to contact and attach to the surface over 45-55 min, we measured in the QCM device a continuous decrease in frequency and continuous increase in resistance, achieving a maximum at about one hr (1400 Ω frequency change and 1400 Ω motional resistance change). These frequency and resistance values stabilized over the next 24 hrs and were unchanged out to 72 hr by QCM measurement (to ∼700 Hz, ∼700 Ω), as the cells were observed to spread in the mock device. Both bovine aortic (BAE) and bovine capillary (BCE) endothelial cells were studied and found to exhibit similar behavior. These studies demonstrate that QCM can be used to detect continuous changes in cell mass and viscoelastic behavior.

scholarly journals Effect of Ionic Strength on Initial Interactions ofEscherichia coli with Surfaces, Studied On-Line by a Novel Quartz Crystal Microbalance Technique

1999 ◽  
Vol 181 (17) ◽  
pp. 5210-5218 ◽  
Author(s):  
Karen Otto ◽  
Hans Elwing ◽  
Malte Hermansson
Keyword(s):  
Ionic Strength ◽  
Quartz Crystal Microbalance ◽  
Direct Contact ◽  
Quartz Crystal ◽  
Contact Point ◽  
Viscoelastic Behavior ◽  
Bacterial Cells ◽  
Hydrophobic Surfaces ◽  
Intimate Contact ◽  
Cell Surface Structures

ABSTRACT A novel quartz crystal microbalance (QCM) technique was used to study the adhesion of nonfimbriated and fimbriated Escherichia coli mutant strains to hydrophilic and hydrophobic surfaces at different ionic strengths. This technique enabled us to measure both frequency shifts (Δf), i.e., the increase in mass on the surface, and dissipation shifts (ΔD), i.e., the viscoelastic energy losses on the surface. Changes in the parameters measured by the extended QCM technique reflect the dynamic character of the adhesion process. We were able to show clear differences in the viscoelastic behavior of fimbriated and nonfimbriated cells attached to surfaces. The interactions between bacterial cells and quartz crystal surfaces at various ionic strengths followed different trends, depending on the cell surface structures in direct contact with the surface. While Δf and ΔD per attached cell increased for nonfimbriated cells with increasing ionic strengths (particularly on hydrophobic surfaces), the adhesion of the fimbriated strain caused only low-level frequency and dissipation shifts on both kinds of surfaces at all ionic strengths tested. We propose that nonfimbriated cells may get better contact with increasing ionic strengths due to an increased area of contact between the cell and the surface, whereas fimbriated cells seem to have a flexible contact with the surface at all ionic strengths tested. The area of contact between fimbriated cells and the surface does not increase with increasing ionic strengths, but on hydrophobic surfaces each contact point seems to contribute relatively more to the total energy loss. Independent of ionic strength, attached cells undergo time-dependent interactions with the surface leading to increased contact area and viscoelastic losses per cell, which may be due to the establishment of a more intimate contact between the cell and the surface. Hence, the extended QCM technique provides new qualitative information about the direct contact of bacterial cells to surfaces and the adhesion mechanisms involved.

Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal

Effect of Current Distribution on Quartz Crystal Microbalance Measurements

1998 ◽  
Vol 145 (2) ◽  
pp. 492-497 ◽  
Author(s):  
James J. Kelly ◽  
K. M. Anisur Rahman ◽  
Christopher J. Durning ◽  
Alan C. West
Keyword(s):  
Quartz Crystal Microbalance ◽  
Current Distribution ◽  
Quartz Crystal
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