Employing Two Different Quartz Crystal Microbalance Models To Study Changes in Viscoelastic Behavior upon Transformation of Lipid Vesicles to a Bilayer on a Gold Surface

2007 ◽  
Vol 79 (18) ◽  
pp. 7027-7035 ◽  
Author(s):  
Nam-Joon Cho ◽  
Kay K. Kanazawa ◽  
Jeffrey S. Glenn ◽  
Curtis W. Frank
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Viscoelastic Behavior ◽  
Lipid Vesicles ◽  
Gold Surface

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

Cellular Adhesion and Spreading of Endothelial Cells Monitored in Real Time Using the Quartz Crystal Microbalance

1998 ◽  
Vol 550 ◽  
Author(s):  
Tiean Zhou ◽  
Susan J. Braunhut ◽  
Diane Medeiros ◽  
Kenneth A. Marx
Keyword(s):  
Endothelial Cells ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Cell Mass ◽  
Viscoelastic Behavior ◽  
Cellular Adhesion ◽  
Frequency Change ◽  
Resistance Change ◽  
Continuous Increase ◽  
Mock Cell

AbstractWe have applied the Quartz Crystal Microbalance (QCM) technique to continuously record the processes of endothelial cell (EC) adhesion, spreading and cellular mass distribution changes during initial cell to surface contact and homeostatic attachment. ECs (50,000) were layered onto a set volume of media in the QCM device and simultaneously in a mock cell used for photomicroscopy. As cells were observed in the mock cell device to contact and attach to the surface over 45-55 min, we measured in the QCM device a continuous decrease in frequency and continuous increase in resistance, achieving a maximum at about one hr (1400 Ω frequency change and 1400 Ω motional resistance change). These frequency and resistance values stabilized over the next 24 hrs and were unchanged out to 72 hr by QCM measurement (to ∼700 Hz, ∼700 Ω), as the cells were observed to spread in the mock device. Both bovine aortic (BAE) and bovine capillary (BCE) endothelial cells were studied and found to exhibit similar behavior. These studies demonstrate that QCM can be used to detect continuous changes in cell mass and viscoelastic behavior.

Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique

Biomaterials ◽  
2006 ◽  
Vol 27 (33) ◽  
pp. 5748-5754 ◽  
Author(s):  
Akira Monkawa ◽  
Toshiyuki Ikoma ◽  
Shunji Yunoki ◽  
Tomohiko Yoshioka ◽  
Junzo Tanaka ◽  
...  
Keyword(s):  
Thin Layer ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Gold Surface

scholarly journals Effect of Ionic Strength on Initial Interactions ofEscherichia coli with Surfaces, Studied On-Line by a Novel Quartz Crystal Microbalance Technique

1999 ◽  
Vol 181 (17) ◽  
pp. 5210-5218 ◽  
Author(s):  
Karen Otto ◽  
Hans Elwing ◽  
Malte Hermansson
Keyword(s):  
Ionic Strength ◽  
Quartz Crystal Microbalance ◽  
Direct Contact ◽  
Quartz Crystal ◽  
Contact Point ◽  
Viscoelastic Behavior ◽  
Bacterial Cells ◽  
Hydrophobic Surfaces ◽  
Intimate Contact ◽  
Cell Surface Structures

ABSTRACT A novel quartz crystal microbalance (QCM) technique was used to study the adhesion of nonfimbriated and fimbriated Escherichia coli mutant strains to hydrophilic and hydrophobic surfaces at different ionic strengths. This technique enabled us to measure both frequency shifts (Δf), i.e., the increase in mass on the surface, and dissipation shifts (ΔD), i.e., the viscoelastic energy losses on the surface. Changes in the parameters measured by the extended QCM technique reflect the dynamic character of the adhesion process. We were able to show clear differences in the viscoelastic behavior of fimbriated and nonfimbriated cells attached to surfaces. The interactions between bacterial cells and quartz crystal surfaces at various ionic strengths followed different trends, depending on the cell surface structures in direct contact with the surface. While Δf and ΔD per attached cell increased for nonfimbriated cells with increasing ionic strengths (particularly on hydrophobic surfaces), the adhesion of the fimbriated strain caused only low-level frequency and dissipation shifts on both kinds of surfaces at all ionic strengths tested. We propose that nonfimbriated cells may get better contact with increasing ionic strengths due to an increased area of contact between the cell and the surface, whereas fimbriated cells seem to have a flexible contact with the surface at all ionic strengths tested. The area of contact between fimbriated cells and the surface does not increase with increasing ionic strengths, but on hydrophobic surfaces each contact point seems to contribute relatively more to the total energy loss. Independent of ionic strength, attached cells undergo time-dependent interactions with the surface leading to increased contact area and viscoelastic losses per cell, which may be due to the establishment of a more intimate contact between the cell and the surface. Hence, the extended QCM technique provides new qualitative information about the direct contact of bacterial cells to surfaces and the adhesion mechanisms involved.

scholarly journals Understanding the Dehydration Stress in Lipid Vesicles by a Combined Quartz Crystal Microbalance and Dielectric Spectroscopy Study

2020 ◽  
Vol 217 (13) ◽  
pp. 1900986
Author(s):  
Alessia Gennaro ◽  
Olivier Deschaume ◽  
Helge Pfeiffer ◽  
Carmen Bartic ◽  
Patrick Wagner ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Dielectric Spectroscopy ◽  
Quartz Crystal ◽  
Lipid Vesicles ◽  
Dehydration Stress ◽  
Spectroscopy Study

Chiral Recognition on Bare Gold Surface by Quartz Crystal Microbalance

2021 ◽  
Author(s):  
Xiangyun Xiao ◽  
Chao Chen ◽  
Yehao Zhang ◽  
Huihui Kong ◽  
Rong An ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Chiral Recognition ◽  
Quartz Crystal ◽  
Gold Surface ◽  
Bare Gold

Synthesizing Zinc Sulfide Films on the Gold Surface as the Sensor for Electrochemical Quartz Crystal Microbalance

2021 ◽  
Vol 57 (12) ◽  
pp. 1157-1163
Author(s):  
D. O. Krinitsyn ◽  
A. S. Romanchenko ◽  
S. A. Vorob’ev ◽  
M. N. Likhatskii ◽  
A. A. Karacharov ◽  
...  
Keyword(s):  
Zinc Sulfide ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Gold Surface ◽  
Electrochemical Quartz Crystal Microbalance

Simultaneous anomalous reflection and quartz-crystal microbalance measurements of protein bindings on a gold surface

2007 ◽  
pp. 3574 ◽  
Author(s):  
Yuichi Manaka ◽  
Yukihiko Kudo ◽  
Hiroshi Yoshimine ◽  
Takayoshi Kawasaki ◽  
Kotaro Kajikawa ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Gold Surface ◽  
Anomalous Reflection
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