Microbial Transformation ofsec-Hydroxyl Group of Polyols into Carbonyl Derivatives by Specific Oxidation Using Methanol Yeast

1991 ◽  
Vol 20 (4) ◽  
pp. 729-730 ◽  
Author(s):  
Shuichi Matsumura ◽  
Tomoko Kawamori ◽  
Sadao Yoshikawa
Keyword(s):  
Microbial Transformation ◽  
Hydroxyl Group ◽  
Carbonyl Derivatives ◽  
Methanol Yeast ◽  
Specific Oxidation

scholarly journals Specific 12β-Hydroxylation of Cinobufagin by Filamentous Fungi

2004 ◽  
Vol 70 (6) ◽  
pp. 3521-3527 ◽  
Author(s):  
Min Ye ◽  
Guiqin Qu ◽  
Hongzhu Guo ◽  
Dean Guo
Keyword(s):  
Microbial Transformation ◽  
Reversed Phase ◽  
In Vitro Models ◽  
In Vitro Cytotoxicity ◽  
New Drugs ◽  
Hydroxyl Group ◽  
Cytotoxic Activities ◽  
Phase Liquid ◽  
New Compounds

ABSTRACT Biotransformation of natural products has great potential for producing new drugs and could provide in vitro models of mammalian metabolism. Microbial transformation of the cytotoxic steroid cinobufagin was investigated. Cinobufagin could be specifically hydroxylated at the 12β-position by the fungus Alternaria alternata. Six products from a scaled-up fermentation were obtained by silica gel column chromatography and reversed-phase liquid chromatography and were identified as 12β-hydroxyl cinobufagin, 12β-hydroxyl desacetylcinobufagin, 3-oxo-12β-hydroxyl cinobufagin, 3-oxo-12β-hydroxyl desacetylcinobufagin, 12-oxo-cinobufagin, and 3-oxo-12α-hydroxyl cinobufagin. The last five products are new compounds. 12β-Hydroxylation of cinobufagin by A. alternata is a fast catalytic reaction and was complete within 8 h of growth with the substrate. This reaction was followed by dehydrogenation of the 3-hydroxyl group and then deacetylation at C-16. Hydroxylation at C-12β also was the first step in the metabolism of cinobufagin by a variety of fungal strains. In vitro cytotoxicity assays suggest that 12β-hydroxyl cinobufagin and 3-oxo-12α-hydroxyl cinobufagin exhibit somewhat decreased but still significant cytotoxic activities. The 12β-hydroxylated bufadienolides produced by microbial transformation are difficult to obtain by chemical synthesis.

Novel biotransformation of menthone by microbial strains and vermicompost microbial consortium

1970 ◽  
Vol 1 ◽  
pp. 204
Author(s):  
Aparna Gayan ◽  
Sonali Sethi ◽  
Om Prakash ◽  
A.K. Pant
Keyword(s):  
Trichoderma Harzianum ◽  
Chemical Structure ◽  
Microbial Consortium ◽  
Microbial Transformation ◽  
Hydroxyl Group ◽  
Ketonic Group ◽  
Microbial Strains ◽  
Different Cultures ◽  
Microbial Strain

The aim of the study was to evaluate the ability of Trichoderma harzianum and Pseudomonas fluorescence and vermicompost microbial consortium to biotransform the monoterpene menthone. The different microbial transformation was carried out in different media including the control flask. Samples of different cultures were taken after every 24 hours, extracted with n-hexane and analysed by GC and GC-MS. The chemical structure of the bio transformed products were identified by GC and GC-MS. All the microbial strains led to the decomposition of menthone with time. The most valuable transformation was the production of menthol by vermicompost microbial strain. The obtained data indicated that vermicompost microbial consortium is a good biocatalyst to convert the ketonic group into hydroxyl group and showed the importance of various microbial strains in the biotransformation of the menthone. 

Specific oxidation of C-14 oxygenated 4(20),11-taxadienes by microbial transformation

2003 ◽  
Vol 44 (5) ◽  
pp. 1091-1094 ◽  
Author(s):  
Jungui Dai ◽  
Shujun Zhang ◽  
Jun-ichi Sakai ◽  
Jiao Bai ◽  
Yoshiki Oku ◽  
...  
Keyword(s):  
Microbial Transformation ◽  
Specific Oxidation

Microbial Transformation of Saponins in Dioscorea zingiberensis for Diosgenin Production with Trichoderma reesei

2013 ◽  
Vol 709 ◽  
pp. 805-809 ◽  
Author(s):  
Li Dan Yu ◽  
Tian Xiang Zheng ◽  
Yu Ling Zhu
Keyword(s):  
Melting Point ◽  
Nmr Spectroscopy ◽  
Trichoderma Reesei ◽  
Microbial Transformation ◽  
Hydroxyl Group ◽  
Dioscorea Zingiberensis ◽  
Sugar Chains ◽  
Hydrolysis Of ◽  
C Nmr

Microbial transformation of plant tubers for diosgenin production is an attractive clean method in industry. The purpose of this study was to evaluate the quality of the product obtained this way. Changes of saponins during the hydrolysis ofDioscorea zingiberensistubers byTrichoderma reeseiwere investigated. Four main saponins were isolated from the plant herbs. Sugar chains, which connected to the C-3 hydroxyl group in saponins, were cleaved stepwisely byTrichoderma reeseifrom the terminal sugar until the aglycone was released. Diosgenin-3-O-β-D-glucose was a byproduct in this process. While, in the petrol extract of the microbial hydrolyzate, only diosgenin was detected. Melting point, IR spectra and13C NMR spectroscopy were used to estimate the quality of the product. Diosgenin obtained from bioconversion showed similar characteristic to that from acid hydrolyzation, confirming microbial transformation was an effective method for diosgenin production.

scholarly journals Microbial Glycosylation of Phenanthrene and Bibenzyls by Mucor hiemalis

2020 ◽  
Vol 15 (11) ◽  
pp. 1934578X2097450
Author(s):  
Bomi Nam ◽  
Ah-Reum Han ◽  
Ik-Soo Lee
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Resolution ◽  
Microbial Transformation ◽  
Hydroxyl Group ◽  
Ionization Mass ◽  
Mass Spectral Data ◽  
Mucor Hiemalis ◽  
Mass Spectral ◽  
Nuclear Magnetic Resonance Spectra ◽  
Microbial Glycosylation

Microbial transformation of denthyrsinin (1), gigantol (2), and batatasin III (3), the major constituents of Dendrobium species (Orchidaceae), was performed using the filamentous fungus Mucor hiemalis KCTC 26779. Three glycosylated metabolites were obtained in the biotransformation of 1-3, and their structures were identified as denthyrsinin-6- O-β-d-glucoside (4), gigantol-5- O-β-d-glucoside (5), and batatasin III-3- O-β-d-glucoside (6) by analyzing 1-dimensional and 2-dimensional-nuclear magnetic resonance spectra, as well as high-resolution electrospray ionization mass spectral data. Among them, metabolite 4 has not been previously reported. Mucor hiemalis was revealed to catalyze enzymatically glucosylation of the hydroxyl group of phenanthrenes and bibenzyls. This research provides an efficient approach for the glycosylation of phenanthrenes and bibenzyls and can expand the library of available phenanthrene and bibenzyl derivatives for further biological evaluations.

Microbial Transformation of 2-Butyn-1,4-diol into 4-Hydroxy-2-butynoic Acid by Specific Oxidation of the Hydroxymethyl Group

1990 ◽  
Vol 19 (4) ◽  
pp. 647-648 ◽  
Author(s):  
Shuichi Matsumura ◽  
Nobuo Yoda ◽  
Masato Endo ◽  
Sadao Yoshikawa
Keyword(s):  
Microbial Transformation ◽  
Hydroxymethyl Group ◽  
Specific Oxidation

Detection method of combustion oscillation characteristics under strong noise background

2020 ◽  
Vol 21 (6) ◽  
pp. 612
Author(s):  
Yunkun Wei ◽  
Tianhong Zhang ◽  
Zhonglin Lin ◽  
Qi Xie ◽  
Yan Zhang
Keyword(s):  
Radiation Thermometry ◽  
Hydroxyl Group ◽  
Interference Signal ◽  
Engine Vibration ◽  
Combustion Technology ◽  
Lean Fuel ◽  
Combustion Oscillation ◽  
Aero Engines ◽  
Temperature Measurement System ◽  
Multispectral Radiation Thermometry

After the lean fuel premixed combustion technology is applied to aero engines, severe combustion oscillations will be cased and led to hidden safety hazards such as engine vibration, further energy waste and other problems. Therefore, it is increasingly important to actively control combustion oscillations. In this paper, a multispectral radiation thermometry (MRT) is used to analyze the hydroxyl group, which is a measurable research object in the combustion chamber of an aero engine, and to fit the functional relationship between the radiation intensity ratio and the temperature in different bands. The theoretical value of the error is <2%. At the same time, in order to solve the problem of weak detection signal and excessive interference signal, an improved frequency domain filtering method based on fast Fourier transform is designed. Besides, the FPGA platform is used to ensure the real-time performance of the temperature measurement system, and simulations and experiments are performed. An oscillating signal with an oscillation frequency of 315 Hz is obtained on the established test platform, and the error is only 1.42%.

TLC/HPTLC and HPLC methods for monitoring microbial transformation processes

2004 ◽  
Vol 17 (2) ◽  
pp. 132-136 ◽  
Author(s):  
Balázs Erdélyi ◽  
Antal Szabó ◽  
László Birincsik ◽  
Gábor Seres ◽  
János Salát ◽  
...  
Keyword(s):  
Microbial Transformation ◽  
Transformation Processes
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