Improved Singlet Oxygen Detection Sensitivity in Electron Spin Resonance Using a Spin-trap Agent Incorporated into a Water-soluble Polymer Film

2018 ◽  
Vol 47 (9) ◽  
pp. 1191-1193
Author(s):  
Kazuki Hosoya ◽  
Saranya Yenchit ◽  
Yuta Tadokoro ◽  
Kei Oya ◽  
Satoru Iwamori
Keyword(s):  
Electron Spin Resonance ◽  
Singlet Oxygen ◽  
Polymer Film ◽  
Electron Spin ◽  
Spin Trap ◽  
Water Soluble ◽  
Detection Sensitivity ◽  
Water Soluble Polymer ◽  
Oxygen Detection ◽  
Spin Resonance

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

Exploring environment-dependent effects of Pd nanostructures on reactive oxygen species (ROS) using electron spin resonance (ESR) technique: implications for biomedical applications

2015 ◽  
Vol 17 (38) ◽  
pp. 24937-24943 ◽  
Author(s):  
Tao Wen ◽  
Weiwei He ◽  
Yu Chong ◽  
Yi Liu ◽  
Jun-Jie Yin ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Electron Spin Resonance ◽  
Singlet Oxygen ◽  
Electron Spin ◽  
Biomedical Applications ◽  
Reactive Oxygen ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Spin Resonance ◽  
Ph Dependent

Pd nanostructures can promote the decomposition of H2O2 in a pH-dependent manner and scavenge superoxide and singlet oxygen.

Electron spin resonance and spin trap investigation of free radicals in cigarette smoke: development of a quantification procedure

2003 ◽  
Vol 481 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Sarah L Baum ◽  
Ian G.M Anderson ◽  
Richard R Baker ◽  
Damien M Murphy ◽  
Christopher C Rowlands
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Cigarette Smoke ◽  
Electron Spin ◽  
Spin Trap ◽  
Spin Resonance
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