Graphene Oxide-based Amplified Fluorescence Sensor for Nucleic Acid Detection through Target-catalyzed Hairpin Assembly

2015 ◽  
Vol 44 (10) ◽  
pp. 1353-1355 ◽  
Author(s):  
Yusuke Kitamura ◽  
Takaaki Miyahata ◽  
Hirotaka Matsuura ◽  
Kazuto Hatakeyama ◽  
Takaaki Taniguchi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acid ◽  
Fluorescence Sensor ◽  
Nucleic Acid Detection ◽  
Catalyzed Hairpin Assembly

A biosensor for the detection of single base mismatches in microRNA

2015 ◽  
Vol 51 (78) ◽  
pp. 14597-14600 ◽  
Author(s):  
Jieon Lee ◽  
Ginam Park ◽  
Dal-Hee Min
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Single Base ◽  
Target Probe

Graphene oxide enables highly sequence specific nucleic acid detection by selectively removing the signal from a mismatched target/probe duplex.

scholarly journals Optical Graphene-Based Biosensor for Nucleic Acid Detection; Influence of Graphene Functionalization and Ionic Strength

2018 ◽  
Vol 19 (10) ◽  
pp. 3230 ◽  
Author(s):  
Diana Becheru ◽  
George Vlăsceanu ◽  
Adela Banciu ◽  
Eugeniu Vasile ◽  
Mariana Ioniţă ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Graphene Oxide ◽  
Ionic Strength ◽  
Nucleic Acid ◽  
Molecular Interaction ◽  
Rational Design ◽  
Nucleic Acid Detection ◽  
Fluorescence Signal ◽  
Main Challenge ◽  
Graphene Functionalization

A main challenge for optical graphene-based biosensors detecting nucleic acid is the selection of key parameters e.g. graphenic chemical structure, nanomaterial dispersion, ionic strength, and appropriate molecular interaction mechanisms. Herein we study interactions between a fluorescein-labelled DNA (FAM-DNA) probe and target single-stranded complementary DNA (cDNA) on three graphenic species, aiming to determine the most suitable platform for nucleic acid detection. Graphene oxide (GO), carboxyl graphene (GO-COOH) and reduced graphene oxide functionalized with PEGylated amino groups (rGO-PEG-NH2, PEG (polyethylene glycol)) were dispersed and characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The influence of ionic strength on molecular interaction with DNA was examined by fluorescence resonance energy transfer (FRET) comparing fluorescence intensity and anisotropy. Results indicated an effect of graphene functionalization, dispersion and concentration-dependent quenching, with GO and GO-COOH having the highest quenching abilities for FAM-DNA. Furthermore, GO and GO-COOH quenching was accentuated by the addition of either MgCl2 or MgSO4 cations. At 10 mM MgCl2 or MgSO4, the cDNA induced a decrease in fluorescence signal that was 2.7-fold for GO, 3.4-fold for GO-COOH and 4.1-fold for rGO-PEG-NH2. Best results, allowing accurate target detection, were observed when selecting rGO-PEG-NH2, MgCl2 and fluorescence anisotropy as an advantageous combination suitable for nucleic acid detection and further rational design biosensor development.

Target-triggered autonomous assembly of DNA polymer chains and its application in colorimetric nucleic acid detection

2016 ◽  
Vol 4 (19) ◽  
pp. 3191-3194 ◽  
Author(s):  
Jianyuan Dai ◽  
Hongfei He ◽  
Zhijuan Duan ◽  
Cuisong Zhou ◽  
Yuyin Long ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Self Assembly ◽  
Polymer Chains ◽  
Nucleic Acid Detection ◽  
One Dimensional ◽  
Autonomous Assembly ◽  
Nucleic Acid Assay ◽  
Sticky End ◽  
Catalyzed Hairpin Assembly

One-dimensional DNA polymer chains were obtained based on the catalyzed hairpin assembly and sticky end self-assembly, which led to a signal amplified colorimetric nucleic acid assay.

Nucleic acid detection on DNA chips using highly reactive and stable diazo-biotins

2008 ◽  
Author(s):  
Alain Laurent ◽  
Arnaud Burr ◽  
Thibault Martin ◽  
Frédéric Lasnet ◽  
Sébastien Hauser ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Dna Chips

scholarly journals Polyethylene Glycol Functionalized Graphene Oxide Nanoparticles Loaded with Nigella sativa Extract: A Smart Antibacterial Therapeutic Drug Delivery System

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3067
Author(s):  
Mustafa A. Jihad ◽  
Farah T. M. Noori ◽  
Majid S. Jabir ◽  
Salim Albukhaty ◽  
Faizah A. AlMalki ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Grain Size ◽  
Cell Wall ◽  
Graphene Oxide ◽  
Polyethylene Glycol ◽  
Nucleic Acid ◽  
Drug Delivery System ◽  
Delivery System ◽  
Nigella Sativa ◽  
Seed Extract

Flaky graphene oxide (GO) nanoparticles (NPs) were synthesized using Hummer’s method and then capped with polyethylene glycol (PEG) by an esterification reaction, then loaded with Nigella sativa (N. sativa) seed extract. Aiming to investigate their potential use as a smart drug delivery system against Staphylococcus aureus and Escherichia coli, the spectral and structural characteristics of GO-PEG NPs were comprehensively analyzed by XRD, AFM, TEM, FTIR, and UV- Vis. XRD patterns revealed that GO-PEG had different crystalline structures and defects, as well as a higher interlayer spacing. AFM results showed GONPs with the main grain size of 24.41 nm, while GONPs–PEG revealed graphene oxide aggregation with the main grain size of 287.04 nm after loading N. sativa seed extract, which was verified by TEM examination. A strong OH bond appeared in FTIR spectra. Furthermore, UV- Vis absorbance peaks at (275, 284, 324, and 327) nm seemed to be correlated with GONPs, GO–PEG, N. sativa seed extract, and GO –PEG- N. sativa extract. The drug delivery system was observed to destroy the bacteria by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell wall integrity, nucleic acid damage, and increased cell-wall permeability.

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