Nucleic Acid Detection of Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2): A Research in the Field of Health Economics

2020 ◽  
Author(s):  
Yanfen Zeng ◽  
Min Dou ◽  
Yaqian Mao ◽  
Yao Li ◽  
Xijun Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Health Economics ◽  
Severe Acute Respiratory Syndrome ◽  
Respiratory Syndrome Virus ◽  
Nucleic Acid Detection

scholarly journals The application of a testing platform in high-throughput nucleic acid detection of SARS-CoV-2

2020 ◽  
Author(s):  
Chen Liu ◽  
Biao Huang ◽  
Rongfang Zhou ◽  
wu hao ◽  
Ming Qiao ◽  
...  
Keyword(s):  
Quality Control ◽  
Nucleic Acid ◽  
Severe Acute Respiratory Syndrome ◽  
Sample Preparation ◽  
High Throughput ◽  
Extraction Method ◽  
Large Scale ◽  
Control Measures ◽  
Nucleic Acid Detection ◽  
Qrt Pcr

Background. The coronavirus disease (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This coronavirus disease has spread globally as a pandemic. Quantitative reverse transcription PCR (qRT-PCR) is established as a diagnostic assay to detect SARS-CoV-2 in patient specimens. The increasing numbers of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed great challenges for the test laboratories.Methods. The test specimens were collected by the hospitals or CDC in Hubei province. Viral RNA is extracted by manual column extraction and automated magnetic bead extraction method by MGISP-960 automated sample preparation system. qRT-PCR for the novel coronavirus is used in this process and several approaches were applied to prevent contamination and monitor process quality.Results. We introduced a high-throughput nucleic acid detection mode, Huo-Yan Laboratory, which has an automated process and rigorous quality control measures to ensure the stability and reliability of detection in large-scale specimens. The automated extraction method based on MGISP-960 sample preparation system has comparable results of manual extraction, and requires less time and personnel. The quality control monitoring results suggests that the quality control approaches in testing large-scale specimens are effective, and the test results are stable and reliable. Huo-Yan Laboratory mode can be flexibly adapted to the requirement of different throughput which is contributed to epidemic control in various places.

Nucleic acid detection on DNA chips using highly reactive and stable diazo-biotins

2008 ◽  
Author(s):  
Alain Laurent ◽  
Arnaud Burr ◽  
Thibault Martin ◽  
Frédéric Lasnet ◽  
Sébastien Hauser ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Dna Chips

Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

Open Medicine ◽  
2007 ◽  
Vol 2 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Koray Ergunay ◽  
Gulcin Altinok ◽  
Bora Gurel ◽  
Ahmet Pinar ◽  
Arzu Sungur ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
San Francisco ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Parvovirus B19 ◽  
Etiologic Agent ◽  
Hydrops Fetalis ◽  
Nucleic Acid Detection

AbstractIntrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).

scholarly journals Are RNA-Based Tests Sufficient for COVID-19 Diagnosis? An Inspiration of Three Asymptomatic Cases

2021 ◽  
Vol 30 ◽  
pp. 096368972098545
Author(s):  
Tao Hu ◽  
Xiao Liu ◽  
Qinan Yin ◽  
Xingting Duan ◽  
Li Yan
Keyword(s):  
Computed Tomography ◽  
Nucleic Acid ◽  
Virus Infection ◽  
Severe Acute Respiratory Syndrome ◽  
The Other ◽  
Computed Tomography Scan ◽  
Health Authorities ◽  
History Of ◽  
Tomography Scan ◽  
Nucleic Acid Tests

In this work, we discovered a new phenomenon—asymptomatic COVID-19 infection, or covert case, during the pandemic. All the 3 patients had a history of exposure, with no symptoms, and no abnormalities were found in computed tomography scan or lab tests. Except for case 2, the other patients’ severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) nucleic acid tests were negative. But their anti-SARS-COV-2 nucleocapsid antibody showed a dynamic trend, consistent with the process of virus infection and clearance. A growing number of asymptomatic or covert cases need more attention. Lack of surveillance may lead to another outbreak. We hope to demonstrate our cases to attract the attention of governments or health authorities that covert cases should be the focus as well.

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