scholarly journals NMR Study of Amino Acids and Their Derivatives. V. Structures and Formation Constants of Zinc-L-Aspartic Acid Complexes in Aqueous Solution

1973 ◽  
Vol 46 (2) ◽  
pp. 468-471 ◽  
Author(s):  
Hidehiro Ishizuka ◽  
Takeo Yamamoto ◽  
Yoji Arata ◽  
Shizuo Fujiwara
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Aspartic Acid ◽  
Formation Constants ◽  
Nmr Study

Formation constants and coordination thermodynamics for binary complexes of Cu(II) and some α-amino acids in aqueous solution

2010 ◽  
Vol 64 (2) ◽  
pp. 281-292 ◽  
Author(s):  
A. Seza Bastug ◽  
Seda E. Goz ◽  
Yesim Talman ◽  
Sinem Gokturk ◽  
Elvan Asil ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Formation Constants ◽  
Binary Complexes

Biochemistry of water-logged soils: Part II. The presence of a deaminase in water-logged soils and its rôle in the production of ammonia

1927 ◽  
Vol 17 (4) ◽  
pp. 449-467 ◽  
Author(s):  
V. Subrahmanyan
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Surface Water ◽  
Aspartic Acid ◽  
Biological Action ◽  
Plant Food ◽  
Soil Sediment ◽  
Dry Out ◽  
Active Preparation ◽  
Production Of Ammonia

(1) The ammonia formed in water-logged soils was present mostly in the soil sediment. The surface water contained only a very small portion of the total amount produced.(2) Added ammonia was in a similar manner retained mostly in the soil itself. It could not be leached out by extraction with water nor volatilised with increase of temperature. There is evidence to show that the ammonia exists in the soil as an exchangeable base.(3) On allowing the soils to dry out the ammonia disappeared rapidly and corresponding amounts of nitrates were formed. Very little ammonia was lost by volatilisation.(4) The production of ammonia took place even in presence of volatile antiseptics. The reaction was shown to be brought about by a deaminase.(5) Studies with a number of proteins and ammo acids showed that only very simple amino compounds (glycine aspartic acid and asparagine) were deaminised. Witte's peptone, which contains amino acids, was also attacked.(6) An active preparation of enzyme was extracted from the soil with an aqueous solution of glycerin saturated with toluene.(7) Significant deaminising action was shown by the enzymes from cultures of the mixed microflora of the soils.(8) By acting on amino bodies that are otherwise resistant to biological action the deaminase probably helps to release readily available plant food. Its action should be of great importance in tropical swamp soils.

Enzymatic Synthesis of 15N-L-aspartic Acid Using Recombinant Aspartase from Escherichia Coli K12

2008 ◽  
Vol 59 (11) ◽  
Author(s):  
Iulia Lupan ◽  
Sergiu Chira ◽  
Maria Chiriac ◽  
Nicolae Palibroda ◽  
Octavian Popescu
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Enzymatic Synthesis ◽  
Large Scale ◽  
Recombinant Dna ◽  
Industrial Enzymes ◽  
Recombinant Dna Technology ◽  
Bacterial Fermentation ◽  
Natural Protein ◽  
Novel Method

Amino acids are obtained by bacterial fermentation, extraction from natural protein or enzymatic synthesis from specific substrates. With the introduction of recombinant DNA technology, it has become possible to apply more rational approaches to enzymatic synthesis of amino acids. Aspartase (L-aspartate ammonia-lyase) catalyzes the reversible deamination of L-aspartic acid to yield fumaric acid and ammonia. It is one of the most important industrial enzymes used to produce L-aspartic acid on a large scale. Here we described a novel method for [15N] L-aspartic synthesis from fumarate and ammonia (15NH4Cl) using a recombinant aspartase.

Post-proline endopeptidase, further characterization of the enzyme from pig kidneys

1984 ◽  
Vol 49 (8) ◽  
pp. 1846-1853 ◽  
Author(s):  
Karel Hauzer ◽  
Tomislav Barth ◽  
Linda Servítová ◽  
Karel Jošt
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Relative Molecular Mass ◽  
Enzyme Molecule ◽  
Thiol Compounds ◽  
Modified Method ◽  
N Terminus

A post-proline endopeptidase (EC 3.4.21.26) was isolated from pig kidneys using a modified method described earlier. The enzyme was further purified by ion exchange chromatography on DEAE-Sephacel. The final product contained about 95% of post-proline endopeptidase. The enzyme molecule consisted of one peptide chain with a relative molecular mass of 65 600 to 70 000, containing a large proportion of acidic and alifatic amino acids (glutamic acid, aspartic acid and leucine) and the N-terminus was formed by aspartic acid or asparagine. In order to prevent losses of enzyme activity, thiol compounds has to be added.

Equilibrium Studies of Binary and Ternary Complexes of Palladium(II) Involving Amino Acids

1993 ◽  
Vol 58 (5) ◽  
pp. 1103-1108 ◽  
Author(s):  
Mohamed M. Shoukry ◽  
Eman M. Shoukry
Keyword(s):  
Amino Acids ◽  
Relative Stability ◽  
Ternary Complex ◽  
Formation Constants ◽  
Ternary Complexes ◽  
Conductivity Measurements ◽  
Equilibrium Studies ◽  
Binary And Ternary Complexes ◽  
Binary Complexes

The formation constants of the binary and ternary complexes of palladium(II) with diethylenetriamine and amino acids as ligands have been determined potentiometrically at 25 °C in 0.1 M NaNO3 solution. The relative stability of each ternary complex was compared with that of the corresponding binary complexes in terms of ∆logK values. The mode of chelation was ascertained by conductivity measurements.

The potential mechanism of atmospheric new particle formation involving amino acids with multiple functional groups

2021 ◽  
Author(s):  
Jiarong Liu ◽  
Ling Liu ◽  
Hui Rong ◽  
Xiuhui Zhang
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Functional Groups ◽  
Atmospheric Aerosols ◽  
Potential Role ◽  
Particle Formation ◽  
Potential Mechanism ◽  
New Particle Formation

Amino acids are recognized as significant components of atmospheric aerosols. However, its potential role in the atmospheric new particle formation (NPF) is poorly understood, especially aspartic acid (ASP), one of...

The Xylem Sap of Maize Roots: Its Collection, Composition and Formation

1988 ◽  
Vol 15 (4) ◽  
pp. 557 ◽  
Author(s):  
MJ Canny ◽  
ME Mccully
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Organic Acid ◽  
Aspartic Acid ◽  
Xylem Sap ◽  
Great Variability ◽  
Reduced Pressure ◽  
Transpiration Stream ◽  
Maize Roots ◽  
Total Amino Acids

Three methods of sampling xylem sap of maize roots were compared: sap bleeding from the stem cut just above the ground; sap bleeding from the cut tops of roots still undisturbed in the ground; and sap aspirated from excavated roots under reduced pressure. The bleeding saps were often unobtainable. When their composition was measured with time from cutting, the concentrations of the major solutes approximately doubled in 2 h. Aspirated sap was chosen as the most reliable sample of root xylem contents. Solute concentrations of the saps showed great variability between individual roots for all solutes, but on average the concentrations found (in �mol g-1 sap) were: total amino acids, 1.8; nitrate, 1.8; sugars (mainly sucrose), 5.4; total organic acids, 18.3. Individual amino acids also varied greatly between roots. Glutamine, aspartic acid and serine were generally most abundant. The principal organic acid found was malic, approximately 8 �mol g-1. From these analyses the ratios of carbon in the fractions (sugars : amino acids : organic acids) = (44 : 6 : 50). 14Carbon pulse fed to a leaf appeared in the root sap within 30 min, rose to a peak at 4-6 h, and declined slowly over a week. During all this time the neutral, cation and anion fractions were sensibly constant in the proportions 86 : 10 : 4. The 14C therefore did not move towards the equilibrium of 12C-compounds in the sap. It is argued that the results do not support a hypothesis of formation of amino carbon from recent assimilate and reduced nitrate in the roots and an export of this to the shoot in the transpiration stream.

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