Water-Soluble Red-Fluorescent Dyes for Two-Photon Deep-Tissue Imaging

2020 ◽  
Vol 93 (10) ◽  
pp. 1226-1233
Author(s):  
Shozo Onishi ◽  
Yasutaka Suzuki ◽  
Hikari Ano ◽  
Jun Kawamata
Keyword(s):  
Fluorescent Dyes ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn
Keyword(s):  
Wavelength Region ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Deep Tissue Imaging

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Extremely High Brightness from Polymer-Encapsulated Quantum Dots for Two-photon Cellular and Deep-tissue Imaging

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yanyan Fan ◽  
Helin Liu ◽  
Rongcheng Han ◽  
Lu Huang ◽  
Hao Shi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
High Brightness ◽  
Two Photon ◽  
Deep Tissue Imaging

Lysosome-targetable polythiophene nanoparticles for two-photon excitation photodynamic therapy and deep tissue imaging

2017 ◽  
Vol 5 (20) ◽  
pp. 3651-3657 ◽  
Author(s):  
Shaojing Zhao ◽  
Guangle Niu ◽  
Feng Wu ◽  
Li Yan ◽  
Hongyan Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Tissue Imaging ◽  
Two Photon Excitation

Polythiophene nanoparticles with large TPA cross section and high1O2generation quantum yield have been developed for simultaneous lysosome-targetable fluorescence imaging and photodynamic therapy.

scholarly journals Advantages of Non-degenerate Two-photon Microscopy for Deep Tissue Imaging

2020 ◽  
Vol 118 (3) ◽  
pp. 311a
Author(s):  
Sanaz Sadegh ◽  
Mu-Han Yang ◽  
Christopher Ferri ◽  
Martin Thunemann ◽  
Anna Devor ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals Evaluating different passive optical clearing protocols for two-photon deep tissue imaging in adult intact visceral and neuronal organs

2015 ◽  
Author(s):  
R.C Vlieg ◽  
C Gillespie ◽  
W M Steve Lee
Keyword(s):  
Spinal Cord ◽  
Tissue Imaging ◽  
Grand Challenge ◽  
Deep Tissue ◽  
Direct Evaluation ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
Embryo Tissue ◽  
Endogenous Fluorescence ◽  
First Time

Imaging cellular activities in an entire intact whole organ with light is a grand challenge in optical microscopy. To date, most passive clearing techniques were shown to transform brain, neuronal and embryo tissue into near transparent state for deep tissue imaging. Here, we expand these passive clearing protocol from neuronal tissue (brain and spinal cord) to other visceral organs such as liver and colon and further evaluate their ?depth-clearing performance? based on image contrast of endogenous fluorescence structures. We found that the SeeDB achieve highest depth in brain, 3DISCO is adept at clearing liver and spinal cord and ScaleViewA2 in colon. Overall, 3DISCO clears more rapidly than other agents at a higher cost, while ScaleViewA2 is the most economical but clears at a slower rate. This study, for the first time, provide a direct evaluation of imaging depth, cost and time amongst passive tissue clearing protocols for different intact organs.

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