Synaptogenic activity of the axon guidance molecule Robo2 is critical for hippocampal circuit function

2020 ◽  
Author(s):  
Ana Dorrego-Rivas
Keyword(s):  
Axon Guidance ◽  
Circuit Function ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

scholarly journals Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function

Cell Reports ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 109828
Author(s):  
Heike Blockus ◽  
Sebi V. Rolotti ◽  
Miklos Szoboszlay ◽  
Eugénie Peze-Heidsieck ◽  
Tiffany Ming ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Circuit Function ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

scholarly journals Synaptogenic activity of the axon guidance molecule Robo2 is critical for hippocampal circuit function

2019 ◽  
Author(s):  
Heike Blockus ◽  
Sebastian V. Rolotti ◽  
Miklos Szoboszlay ◽  
Tiffany Ming ◽  
Anna Schroeder ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Pyramidal Neurons ◽  
Synapse Formation ◽  
Molecular Level ◽  
Spatial Coding ◽  
Synaptic Specificity ◽  
Circuit Function ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

The developmental transition between axon guidance and synapse formation is critical for circuit assembly but still poorly understood at the molecular level. We hypothesized that this key transition could be regulated by axon guidance cues switching their function to regulate synaptogenesis with subcellular specificity. Here, we report evidence for such a functional switch, describing a novel role for the axon guidance molecule Robo2 in excitatory synapse formation onto dendrites of CA1 pyramidal neurons (PNs) in the mouse hippocampus. Cell-autonomous deletion of Robo2 from CA1 PNs leads to a drastic reduction of the number of excitatory synapses specifically in proximal dendritic compartments. At the molecular level, we show that this novel postsynaptic function of Robo2 depends on both its canonical ligand Slit and a novel interaction with presynaptic Neurexins. Biophysical analysis reveals that Robo2 binds directly to Neurexins via its Ig4-5 domains. In vivo 2-photon Ca2+ imaging of CA1 PNs during spatial navigation in mice shows that sparse deletion of Robo2 during development drastically reduces the likelihood of place cell emergence and alters spatial coding properties of the remaining place cells. Our results identify Robo2 as a novel molecular effector linking synaptic specificity to the acquisition of spatial coding properties characterizing hippocampal circuits.

The Beat generation: a multigene family encoding IgSF proteins related to the Beat axon guidance molecule in Drosophila

Development ◽  
2001 ◽  
Vol 128 (22) ◽  
pp. 4545-4552 ◽  
Author(s):  
G. C. Teg Pipes ◽  
Qing Lin ◽  
Stephanie E. Riley ◽  
Corey S. Goodman
Keyword(s):  
Axon Guidance ◽  
Specific Growth ◽  
Growth Cones ◽  
Beat Generation ◽  
Genetic Screen ◽  
Genetic Interactions ◽  
Membrane Bound ◽  
Adhesive Factor ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

A previous genetic screen led to the identification of the beaten path (beat Ia) gene in Drosophila. Beat Ia contains two immunoglobulin (Ig) domains and appears to function as an anti-adhesive factor secreted by specific growth cones to promote axon defasciculation. We identify a family of 14 beat-like genes in Drosophila. In contrast to beat Ia, four novel Beat-family genes encode membrane-bound proteins. Moreover, mutations in each gene lead to much more subtle guidance phenotypes than observed in beat Ia. Genetic interactions between beat Ic and beat Ia reveal complementary functions. Our data suggest a model whereby Beat Ic (and perhaps other membrane-bound family members) functions in a pro-adhesive fashion to regulate fasciculation, while Beat Ia (the original secreted Beat) functions in an anti-adhesive fashion to regulate defasciculation.

LOTUS, a novel axon guidance molecule, functions as a negative regulator of Nogo signaling in LOT formation

2009 ◽  
Vol 65 ◽  
pp. S94
Author(s):  
Masumi Iketani ◽  
Megumi Yamaguchi ◽  
Yuko Arie ◽  
Yuji Kurihara ◽  
Yasufumi Sato ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Negative Regulator ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

Abstract 3574: Novel roles of an axon-guidance molecule, semaphorin 3E, in pancreatic cancer

2015 ◽  
Author(s):  
Lin-Kin Yong ◽  
Dali Li ◽  
Ethan Poteet ◽  
Zhengdong Liang ◽  
William Fisher ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Axon Guidance ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

Phenotype of V2-derived interneurons and their relationship to the axon guidance molecule EphA4 in the developing mouse spinal cord

2007 ◽  
Vol 26 (11) ◽  
pp. 2989-3002 ◽  
Author(s):  
Line Lundfald ◽  
C. Ernesto Restrepo ◽  
Simon J. B. Butt ◽  
Chian-Yu Peng ◽  
Steven Droho ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Axon Guidance ◽  
Mouse Spinal Cord ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

Promotion of neurite outgrowth by a novel axon guidance molecule LOTUS

2009 ◽  
Vol 65 ◽  
pp. S94
Author(s):  
Yuji Kurihara ◽  
Yuko Arie ◽  
Megumi Yamaguchi ◽  
Masumi Iketani ◽  
Fumio Nakamura ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Neurite Outgrowth ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

Structural and functional analysis of the axon guidance molecule Sema3A and its receptor

2004 ◽  
Vol 2004 (Fall) ◽  
Author(s):  
Claudia Hoemme ◽  
Jens Schwamborn ◽  
Alexander Antipenko ◽  
Dimitar B. Nikolov ◽  
Andreas W. P�schel
Keyword(s):  
Functional Analysis ◽  
Axon Guidance ◽  
Axon Guidance Molecule ◽  
Guidance Molecule

LOTUS, a novel axon guidance molecule

2009 ◽  
Vol 65 ◽  
pp. S94
Author(s):  
Yasufumi Sato ◽  
Megumi Yamaguchi ◽  
Masumi Iketani ◽  
Yuko Arie ◽  
Yuji Kurihara ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Axon Guidance Molecule ◽  
Guidance Molecule
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