Isosmotic Regulation in Various Tissues of the Diamondback Terrapin Malaclemys Centrata Centrata (Latreille)

1973 ◽  
Vol 59 (1) ◽  
pp. 39-43
Author(s):  
M. GILLES-BAILLIEN

1. Osmotic adjustment is achieved by blood and intracellular fluids in the diamond-back terrapin when acclimatized either to fresh water or to sea water. 2. The muscle adjusts its composition to a higher blood osmotic pressure by greater concentrations in ammonia, in taurine and in urea and to a lesser extent in all amino acids (aspartate excepted). The inorganic ion content is not affected. 3. In the bladder mucosa ammonia, taurine and all amino acids are more concentrated in sea-water animals. But essentially urea is responsible for the higher osmotic pressure. Of the inorganic ions only potassium shows a (slight) increase in sea-water animals. 4. In the colon mucosa there is a slight increase in the total amino acid content, in the concentrations of sodium and chloride, and a larger increase in urea. 5. In the jejunum mucosa the concentrations of amino acids, urea and K are much higher in sea-water animals. 6. The results are discussed within the framework of isosmotic regulation of intracellular fluids.

Author(s):  
Lata . ◽  
Narender Singh Atri

Objective: In this paper amino acid profile of Lentinus sajor-caju (Fr.) Fr., a basidiomycetous mushroom has been investigated.Methods: During the evaluation 15 amino acids (lysine, aspartic acid, serine, threonine, glutamic acid, cysteine, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine and histidine) were determined from the dried sample of Lentinus sajor-caju by following the standard technique of biochemistry using ion-exchange chromatography.Results: The total amino acid content has been evaluated at 18.82 g/100g. Amongst the evaluated amino acids, exogenous amino acid lysine (6.66 g/100g) is preponderantly present in comparison to all other amino acids. The essential amino acid (EAA) index (44.64%) and biological value (BV=36.93%) has also been determined for the examined sample.Conclusion: Lentinus sajor-caju (Fr.) Fr. is a potential source of quality protein with a substantial proportion of exogenous and endogenous amino acids.


2004 ◽  
Vol 79 (2) ◽  
pp. 231-240 ◽  
Author(s):  
A. I. García ◽  
J. C. de Bias ◽  
R. Carabaño

AbstractTwenty-eight New Zealand White X Californian doe rabbits weighing 3791±141 g were surgically fitted with a glass T-cannula at ileum level. Animals were given ad libitum access to either a casein-based diet (C) or a protein-free diet (PF). The only difference in the ingredient composition of the diets was the substitution of 160g/kg of maize starch by casein in the C diet. Animals were randomly allotted to each experimental diet. Seven rabbits per diet were not allowed to practice caecotrophy. The C diet resulted in higher food dry matter (DM) intake (DMI) (111-5 v. 51-5 g/day), food nitrogen intake (3-12 v. 0-20 g/day), ileal flow of DM (56-0 v. 23-8 g/day) and ileal flow of nitrogen (0-72 v. 0-24 g/day) but lower soft faeces DMI (21-7 v. 41-4 g/day) than the PF diet. A linear relationship between ileal endogenous nitrogen flow and total DMI was found for animals given the C but not for the PF diet. The ingestion ofC diet led to a higher (proportionately 0-41, on average), endogenous ileal amino acid flow than PF diet for all amino acids except for glycine. The ileal flow of glycine was proportionately 0-25 higher in animals given the PF diet than in animals given the C diet. The C diet resulted in higher content of nitrogen, threonine and proline in soft faeces than the PF diet. In contrast, the ingestion of the PF diet led to a higher content of lysine and methionine in soft faeces than the C diet. Within the C diet, animals practising caecotrophy showed higher ileal flow of DM (73-4 v. 56-0 g/day) and phenylalanine (0-834 v. 0-677 g/kg DMI) than animals not practising it. Caecotrophy led to a higher endogenous proportion of some of the most important limiting essential amino acids in rabbits, such as arginine, lysine, phenylalanine and threonine. In rabbits, the use of PF diets has limited practical application in the estimation of nitrogen endogenous losses as the low food DMI and the high soft faecesDMI leads to abnormal food: soft faeces intake ratio. Moreover, due to the important effect of caecotrophy on the amino acid composition of endogenous losses, it is important to correct the total amino acid content of the ileal chyme by the undigested amino acid residue coming from the soft faeces intake. This correction could be made, without the methodological complexity implied, by fitting animals with a wooden collar and thereby avoiding caecotrophy as in the present trial.


1968 ◽  
Vol 48 (2) ◽  
pp. 215-220 ◽  
Author(s):  
E. M. Olsen ◽  
S. J. Slinger ◽  
J. D. Summers

Two experiments were conducted with rats to evaluate the protein quality of a series of wheat by-products, which served as the sole source of protein in diets calculated to contain 10% protein. Digestibility of nitrogen and percentage absorption of amino acids were compared with weight gain, feed efficiency and net protein utilization (NPU) as criteria. Percentage absorption of different amino acids was found to vary considerably in the same protein. Of the essential amino acids, lysine, threonine, methionine and isoleucine had the lowest percentage absorption in all of the products tested. The results indicate that the amount of amino acids absorbed, as measured by the excretion method, may be a more satisfactory measure of protein quality than total amino acid content.


OENO One ◽  
1999 ◽  
Vol 33 (2) ◽  
pp. 49
Author(s):  
Corine Larchevêque ◽  
A. Casanova

<p style="text-align: justify;">Numerous studies have been carried out on the vine, must and wine, but few on sap. Main phenological stages i.e. bud burst, the flowering period, veraison and the ripening period have been well described. However, at the present time, no study is available identifying the main amino acids of the xylem sap, at the formentioned periods. Located within the « Graves de Pessac-Léognan » vineyard, the plot area studied has a clayey-chalky soil with a small amount of sand present. The cultivar Cabernet franc was grafted on the Fercal rootstock. Planted in June 1982, the vine was trained with Guyot pruning and no tillage. The density of the plantation was 5.550 vines per ha. Four rows of 21 vines were sampled.</p><p style="text-align: justify;">Analysis of xylem sap, by H.P.L.C., at the four stages, taken from several vintages, showed that the global content of amino acids varied year to year (LARCHEVÊQUE, 1998). The experiment reported on herein was carried out in order to determine both the main amino acids in the xylem sap and to identify a marker amongst them, having the same evolution as that of the global amino acid content from one phenological stage to the next. A pool of eight amino acids, all present in large quantities (about 80 p. cent of total amino acid content), was always noted : asparagine/glutamine, tyrosine, arginine, aspartic acid, glutamic acid, histidine and proline. Concentration levels of these components differed in sap, depending on the vintage, at the same phenological stage. At bud burst, as at the flowering period, the sap had a similar qualitative composition.</p><p style="text-align: justify;">It was noteworthy that aspartic acid evidenced the same evolution that of the total amino acid content (i.e. the same variation in percentage) from one phenological stage to the next. Aspartic acid should therefore be regarded as a « marker » of total amino acid content evolution during the vegetative cycle.</p>


Author(s):  
Artiona Laze ◽  
Valentina Arapi ◽  
Evelina Ceca ◽  
Kristi Gusho ◽  
Lato Pezo ◽  
...  

The purpose of this study was to evaluate the chemical parameters and amino acid content in 10 genotypes of soft Albanian wheat organically grown and collected during the summer seasons of 2014. The thin layer chromatography method was used for the identification the content of amino acids and spectrophotometry was used to determine the concentration of indentified amino acids. The results exhibited high protein content (13.73 % - 17.32 %) and relative high content of total amino acid content (6.3 % - 10.9 %). The most abundant of all amino acids was glutamic acid (in all genotypes) and cysteine (PZA 1, PZA 2, PZA 3, PZA 4, PZA 5, PZA 6, PZA 7, and PZA 10).


1970 ◽  
Vol 48 (11) ◽  
pp. 2033-2036 ◽  
Author(s):  
G. D. Thorn ◽  
L. T. Richardson

Nabam (I), methylmercury dicyandiamide (II), Dexon (III), nonenylsuccinic acid (IV) and its monomethyl ester (V), benomyl, Vitavax, Plantvax, and Uniroyal F849 and G696 were applied as drenches to the soil of potted tomato plants. Exudate collected for 6 h from the stumps of plants detopped 24 h after application of each chemical was markedly reduced in volume and total amino acid content by I–V but not the others. Relative levels of individual amino acids were little affected except that I induced changes in the relative amounts of several primary amino acids, and II increased alanine concentration in the exudates.


2014 ◽  
Vol 884-885 ◽  
pp. 389-393
Author(s):  
Ya Wei ◽  
Yong Qiang Zhao ◽  
Shu Xian Hao ◽  
Jian Wei Cen ◽  
Hui Huang ◽  
...  

Jade perch (Scortum barcoo) meat nutrition analysis and evaluation was carried out in this paper, results showed that: the protein content, fat content and total amino acid content of Jade perch are 18.4%, 9.0% and 19.99% respectively. As a balanced amino acid combination, it contains a variety of amino acids necessary for the meat, glutamic acid content of up to 3.10%, delicious amino acids in the total amino acid content 7.64%. Jade perch has a lower fat content, but rich in unsaturated fatty acids, DHA content is 3.36%, rich in Ca, Fe, Zn, Se and other trace elements, in the jade perch, the heavy metals and other toxic substances analysis showed that they are in line with national health and safety standards, the Jade Perch is a good quality, high nutritional value of edible fish.


Author(s):  
Walid Elfalleh ◽  
Nizar Tlili ◽  
Ma Ying ◽  
He Sheng-Hua ◽  
Ali Ferchichi ◽  
...  

Chemicals from fresh pomegranates were established. Total sugar of juice extracts are mainly fructose (ca. 7.27 g/100 ml) and glucose (ca. 8.11 g/100 ml). Pulp minerals, expressed as mg/100g are potassium (351.26), magnesium (10.02), calcium (9.26), sodium (7.25), iron (0.15), Zinc (0.10), copper (0.08) and manganese (0.04). Juice proteins are about 7.95 g/l and dry pulp proteins are 24% on a dry-weight basis DW (Kjeldhal assays). Seed proteins are ca. 16.87% DW. Globulins (62.4 mg/g DW) and albumins (54.12 mg/g DW) are the major storage protein fractions, followed by glutelins (33.2 mg/g DW) and prolamins (18.08 mg/g DW). Seed total amino acid content is 14.45 g/100 g DW. Seeds are rich in Glu, Arg, Asp, Leu and Gly amino acids. Essential amino acids average 33.54% of total amino acid. Essential amino acids are mostly all much higher than the requirement of FAO/WHO for adults (but none for preschool children). Most other amino acids have content slightly fewer. All of these findings implied that the content of sugar, mineral and protein maybe included in pomegranate as supplements for commonly diets.


OENO One ◽  
2019 ◽  
Vol 53 (1) ◽  
Author(s):  
Teresa Garde-Cerdan ◽  
Gastón Gutiérrez-Gamboa ◽  
Eva Pilar Pérez-Álvarez ◽  
Pilar Rubio-Bretón

Aim: The study of elicitors in grapevines has focused on inducing resistance to diseases and improving phenolic and volatile composition of grapes and wines. Due to the importance of nitrogen compounds in grape quality, the aim of this work was to study the effect of methyl jasmonate (MeJ) applied to two grapevine varieties on grape amino acid content during two consecutive seasons.Methods and results: Amino acids were analyzed by HPLC-DAD. The results showed that MeJ applications to Tempranillo grapevines decreased the concentration of certain amino acids during both seasons, but did not affect total amino acid content. Moreover, MeJ applications to Graciano grapevines increased the concentration of several amino acids during the second season.Conclusions: The effects of MeJ on grape amino acid concentration were conditioned by variety and vintage.Significance and impact of the study: The importance of this work is to provide more information with respect to the effects of elicitors on grape quality for good viticultural management.


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