In Vitro Culture of Embryos in the Silkworm, Bombyx Mori L

1963 ◽  
Vol 40 (4) ◽  
pp. 735-739
Author(s):  
TAKEO TAKAMI
Keyword(s):  
In Vitro Culture ◽  
Bombyx Mori ◽  
Embryo Culture ◽  
Prothoracic Gland ◽  
Prothoracic Glands ◽  
Hormonal Action ◽  
Bombyx Mori L ◽  
The Brain ◽  
Silkworm Bombyx Mori

1. Silkworm embryos decapitated before the differentiation of neuroblasts, or before differentiation of the prothoracic glands, were cultured in vitro. 2. Brainless embryos which developed from these decapitated explants detached the cuticle, suggesting that the ‘moult’ of cultured embryos does not require any hormonal action of the brain in the silkworm at least for its initiation. 3. The prothoracic gland was not found to be necessary even for occurrence of the embryonic moult. 4. The author's results obtained by embryo culture in the silkworm are contrary to those obtained by ligaturing experiments in the locusts. This difference between the two cases present important questions about the nature of the moult in cultured silkworm embryos, which must be answered by further investigations.

In Vitro Culture of Embryos in the Silkworm, Bombyx Mori L

1958 ◽  
Vol 35 (2) ◽  
pp. 286-296
Author(s):  
TAKEO TAKAMI
Keyword(s):  
In Vitro Culture ◽  
Bombyx Mori ◽  
Morphological Change ◽  
Cold Storage ◽  
Diapausing Eggs ◽  
Egg Extracts ◽  
Appendage Formation ◽  
Bombyx Mori L ◽  
Silkworm Bombyx Mori

1. Experiments have been carried out on the in vitro culture of silkworm embryos with silkworm egg extracts. 2. Non-diapausing embryos can be cultured well beyond the stage of appendage formation with the extract from non-diapausing eggs, while fully diapausing embryos at about the 30th day are, for the most part, unable to grow in the same extract. 3. 1-2-day-old diapausing embryos still retain the ability to develop in vitro as well as non-diapausing ones. 4. The extract from 1-2-day-old diapausing eggs has nearly the same nutritive effect on the in vitro culture of embryos as that from non-diapausing eggs, though the extract becomes less nutritive with age of eggs. 5. Diapause of both embryo and yolk can almost be prevented by cold-storage at 5° C. 6. The yolk of diapausing eggs kept continuously at 25° C. possibly recovers, after about 150 days, its nutritive effect, which falls with the onset of diapause. This change is independent of activation of the embryo, and is accompanied by little morphological change in the yolk cells.

Studies on the Mode of Action of the Diapause Hormone in the Silkworm, BOMBYX MORI L.,

1963 ◽  
Vol 40 (3) ◽  
pp. 517-530
Author(s):  
KINSAKU HASEGAWA
Keyword(s):  
Low Temperature ◽  
Juvenile Hormone ◽  
Bombyx Mori ◽  
Mode Of Action ◽  
Adult Emergence ◽  
Suboesophageal Ganglion ◽  
Bombyx Mori L ◽  
Diapause Hormone ◽  
The Brain ◽  
Silkworm Bombyx Mori

1. The action of the diapause hormone has been studied by injecting extracts of the heads of male moths or of the brain-suboesophageal ganglion complexes of pupae into pupae expected to produce non-diapause eggs. 2. The effect of the injection of hormone upon young oocytes is to make them develop into diapause eggs. Older oocytes, which have already acquired non-diapause characters, are not affected. 3. The hormone is almost completely inactivated when injected on the day of pupation. The hormone is most effective when injected into pupae 2-3 days old, at which stage the ovarioles have started to grow vigorously. It is ineffective 1-2 days before adult emergence, by which time all the oocytes have acquired non-diapause characters. 4. The hormone is inactivated in all pupae irrespective of whether they are destined to produce diapause eggs or non-diapause eggs. Inactivation of diapause hormone (in contrast to that of juvenile hormone) is partially relieved by exposure to low temperature or by simultaneous injection of indian ink. 5. The extracts prepared as in (1) above do not serve as a stimulant for the brain causing the suboesophageal ganglion to produce diapause hormone. The action of the extract faithfully reflects the function of the diapause hormone which originates in the suboesophageal ganglion.

In vitro action of prothoracicotropic hormone on RNA-synthesis in the prothoracic gland of the silkworm, Bombyx mori

1986 ◽  
Vol 32 (8) ◽  
pp. 711-717 ◽  
Author(s):  
Yasuo Aizono ◽  
Noboru Matsuo ◽  
Yoshihiro Yoshida ◽  
Gunki Funatsu ◽  
Masaru Funatsu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Rna Synthesis ◽  
Prothoracic Gland ◽  
Prothoracicotropic Hormone ◽  
Silkworm Bombyx Mori

Proteomic identification of the silkworm (Bombyx mori L) prothoracic glands during the fifth instar stage

2009 ◽  
Vol 29 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Xiao-feng Wu ◽  
Xing-hua Li ◽  
Wan-fu Yue ◽  
Bhaskar Roy ◽  
Guang-li Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Cell Function ◽  
Storage Proteins ◽  
Primary Source ◽  
Prothoracic Gland ◽  
Japanese Strain ◽  
Proteomic Approach ◽  
Bombyx Mori L ◽  
The Difference ◽  
Silkworm Bombyx Mori

Although the ecdysteroid of the silkworm had been studied for decades, the proteome of the prothoracic gland, the primary source of ecdysteroid hormones, has not been studied previously. In the present paper, we utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25–65 kDa, with pI values of between 4 and 7, and the difference was not distinct. When compared with Qiufeng (Japanese strain), the interspecific distinction was larger than the intraspecific distinction, and 19 particular spots, excized from the third, fifth and ninth days of p50 (Chinese strain) and Qiufeng were subjected to MALDI-TOF–MS (matrix-assisted laser-desorption ionization–time-of-flight MS) analysis. We sorted them into seven catagories: energetics and/or metabolism, storage proteins, protection, lipid metabolism, signal transduction, cell function and unknown function proteins. Of these proteins, arginine methyltransferase is discussed as playing an important role in regulating the activation of ecdysteroidogenesis via transcription or translation.

The ‘Brain’ Hormone in the Silkworm, Bombyx mori L.

Nature ◽  
1958 ◽  
Vol 181 (4617) ◽  
pp. 1217-1217 ◽  
Author(s):  
MASATOSHI KOBAYASHI ◽  
JIRO KIRIMURA
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori L ◽  
The Brain ◽  
Silkworm Bombyx Mori
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