scholarly journals The adrenergic responses of carp (Cyprinus carpio) red cells: effects of PO2 and pH

1988 ◽  
Vol 136 (1) ◽  
pp. 405-416 ◽  
Author(s):  
A. Salama ◽  
M. Nikinmaa
Keyword(s):  
Intracellular Ph ◽  
Oxygen Content ◽  
Oxygen Tension ◽  
Cyprinus Carpio ◽  
Ph Value ◽  
Atmospheric Oxygen ◽  
Red Cells ◽  
Red Cell ◽  
Root Effect ◽  
Carp Cyprinus Carpio

Carp (Cyprinus carpio) red cells do not show beta-adrenergic responses when incubated with 10(−5) mol l-1 adrenaline at atmospheric oxygen tension and a pH value close to the in vivo resting pH (approx. 8.1). However, when either the pH or the oxygen tension of the incubation medium is decreased, the adrenergic responses appear, showing that oxygen or an oxygen-linked phenomenon has a direct influence on the response. Once present, the adrenergic red cell response is similar to that of trout: cellular water content, sodium content and intracellular pH all increase. Quantitatively the effect appears to be much smaller in carp than in trout. Adrenaline induces an increase in red cell oxygen content when the oxygen content is plotted as a function of extracellular pH. This effect coincides with the onset of the Root effect and is caused by the adrenaline-induced increase in intracellular pH, since it disappears when the oxygen content is plotted as a function of intracellular pH. The red cell ATP content decreases metabolically during adrenaline incubations. In contrast, cellular GTP content is not metabolically reduced in adrenaline-treated cells, showing that the rapid and selective decrease in red cell GTP concentration, observed in hypoxic cyprinids, is not adrenergically induced.

scholarly journals Adrenergic Swelling of Nucleated Erythrocytes: Cellular Mechanisms in a Bird, Domestic Goose, and two Teleosts, Striped Bass and Rainbow Trout

1984 ◽  
Vol 113 (1) ◽  
pp. 215-224 ◽  
Author(s):  
MIKKO NIKINMAA ◽  
WRAY H. HUESTIS
Keyword(s):  
Rainbow Trout ◽  
Cell Volume ◽  
Intracellular Ph ◽  
Striped Bass ◽  
Incubation Medium ◽  
Red Cells ◽  
Red Cell ◽  
Volume Changes ◽  
Cellular Mechanisms ◽  
Loosely Coupled

The mechanism of adrenergic swelling and associated pH changes was investigated in avian (goose) and teleost (striped bass and rainbow trout) erythrocytes. The swelling of goose red cells was probably caused by Na+/K+/Cl− co-transport and consecutive osmotic flow of water into the cell. Goose red cells swelled when exposed to isoproterenol in the presence of elevated extracellular K+, but not at physiological K+ concentrations. The swelling was quantitatively inhibited by furosemide, and by removing Cl− from the incubation medium, but was not affected by DIDS. The isoproterenol-induced swelling of fish erythrocytes may be due to loosely coupled Na+/H+ and Cl−/HCO3− exchanges. Furosemide did not completely inhibit the swelling of striped bass red cells. The cell volume increased even if K+ was completely removed from the incubation medium. In contrast, both DIDS and amiloride treatment, and the removal of Na+ from the incubation medium, inhibited the volume changes. In fish red cells the swelling is associated with a clear acidification of the medium and alkalinization of the red cell contents. This phenomenon was most pronounced when the cells were treated simultaneously with DIDS and isoproterenol; the intracellular pH became higher than the extracellular one. Both amiloride and removal of Na+ from the incubation medium prevented the reversal of the transmembrane pH gradient in cells treated simultaneously with DIDS and isoproterenol.

Effect of plasma-activated Water on Shewanella putrefaciens populations growth and quality of Yellow River carp (Cyprinus carpio) fillets

2021 ◽  
Author(s):  
Xiao Liu ◽  
Mingli Zhang ◽  
Xi Meng ◽  
Yanhong Bai ◽  
Xiuping Dong
Keyword(s):  
Cyprinus Carpio ◽  
Ph Value ◽  
Yellow River ◽  
B Value ◽  
Shewanella Putrefaciens ◽  
Deionized Water ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Yellow River Carp ◽  
Plasma Activated Water

Plasma-activated water (PAW) is a new sanitizer, which has received great attention for application in food industries. This research aimed to evaluate the effect of PAW on the inactivation of Shewanella putrefaciens and quality attributes of Yellow River carp ( Cyprinus carpio ) fillets. The carp fillet samples were immersed in sterile deionized water (SDW) or PAW120 (plasma discharge on the deionized water surface for 120 s) for 1.5, 3.0, 4.5, and 6.0 min, respectively. After being treated by PAW120 for 6 min, the population of Shewanella putrefaciens on carp fillets was significantly decreased by 1.03 log 10 CFU/g ( p < 0.05). Compared with SDW-treated samples, the L * value of carp fillets increased, whereas the a * value was decreased following PAW120 treatment, while there was no significant difference in the b * value ( p > 0.05). Compared with SDW, PAW120 caused no significant changes in sensory properties and texture attributes of carp fillets including hardness, springiness, gumminess, and cohesiveness ( p > 0.05). However, for 6-min treatment of PAW120 caused a significant increase in the lipid oxidation level and a decrease in the pH value of the carp fillets. This work provides a basis for the potential application of PAW in the preservation of aquatic products.

scholarly journals Effect of modified atmosphere and vacuum packaging on selected chemical parameters of rainbow trout (Oncorhynchus mykiss) and carp (Cyprinus carpio) cuts freshness

2014 ◽  
Vol 68 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Jelena Babic ◽  
Mirjana Dimitrijevic ◽  
Milan Milijasevic ◽  
Vesna Djordjevic ◽  
Radivoj Petronijevic ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cyprinus Carpio ◽  
Ph Value ◽  
Modified Atmosphere ◽  
Chemical Parameters ◽  
Group Iii ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Group I ◽  
Carp Cyprinus Carpio

The purpose of food packing in modified atmosphere is to extend its sustainability by preventing both biochemical processes and growth of spoilage bacteria. Gases or their mixtures which are mostly used in the modified atmosphere food packing technology are carbon-dioxide (CO2), oxygen (O2) and nitrogen (N2). The aim of our research was to examine the influence of packaging in modified atmosphere and vacuum on the total volatile basic nitrogen (TVB-N) content and pH in muscle of rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio), as well as to determine the most suitable gas mixtures for packing of these freshwater species. Three sample groups of trout and carp cuts were investigated. The first two groups were packaged in modified atmosphere with different gas ratios: 60%CO2+40%N2 (I group) and 40%CO2+60%N2 (II group), whereas the samples from third, control group, (III group) were vacuum packaged. During trials samples were stored in refrigerator at +3?C. Determination of TVB-N and pH was performed on 1st, 7th and 14th day of storage. The obtained results indicate that the investigated mixtures of gases and vacuum as well had a significant influence on the values of TVB-N in trout and carp cuts samples. The lowest increase in TVB-N was established in trout and carp cuts samples from the group I, whereas the highest increase was established in samples from group III. Statistical significant difference (p < 0,001) between the average values of TVB-N for trout (I group: 18,17 ? 0,93; II group: 20,90 ? 0,81 and III group: 36,18 ? 2,65 mg N/100 g ) and carp cuts (I group: 26,74 ? 1,48; II group: 30,02 ? 0,31 and III group: 35,10 ? 1,75 mg N/100 g) was established on 14th day. The lowest pH value was established in samples packaged in modified atmosphere with 60% CO2 +40% N2 (I group). On 14th day of testing the obtained value was 6,15 ? 0,09 for trout and 5,94 ? 1,11 for carp samples. Increase in pH value in trout samples packed in vacuum was established during the whole period of investigation (p < 0,001), while in carp cuts samples packaged in vacuum the increase in pH value (p < 0,05) was established up to 7th day of testing. Based on the obtained results it can be concluded that gas mixture consisting of 60% CO2 and 40% N2 was the most suitable for packaging of fresh trout and carp cuts in terms of selected chemical parameters, such as TVB-N and pH.

scholarly journals The role of WNK in modulation of KCl cotransport activity in red cells from normal individuals and patients with sickle cell anaemia

2019 ◽  
Vol 471 (11-12) ◽  
pp. 1539-1549
Author(s):  
David C.-Y. Lu ◽  
Anke Hannemann ◽  
Rasiqh Wadud ◽  
David C. Rees ◽  
John N. Brewin ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Sickle Cell ◽  
Sickle Cell Anaemia ◽  
Kinase Inhibitors ◽  
Red Cells ◽  
Red Cell ◽  
Sickle Cells ◽  
Kcl Cotransport ◽  
Pre Treatment

AbstractAbnormal activity of red cell KCl cotransport (KCC) is involved in pathogenesis of sickle cell anaemia (SCA). KCC-mediated solute loss causes shrinkage, concentrates HbS, and promotes HbS polymerisation. Red cell KCC also responds to various stimuli including pH, volume, urea, and oxygen tension, and regulation involves protein phosphorylation. The main aim of this study was to investigate the role of the WNK/SPAK/OSR1 pathway in sickle cells. The pan WNK inhibitor WNK463 stimulated KCC with an EC50 of 10.9 ± 1.1 nM and 7.9 ± 1.2 nM in sickle and normal red cells, respectively. SPAK/OSR1 inhibitors had little effect. The action of WNK463 was not additive with other kinase inhibitors (staurosporine and N-ethylmaleimide). Its effects were largely abrogated by pre-treatment with the phosphatase inhibitor calyculin A. WNK463 also reduced the effects of physiological KCC stimuli (pH, volume, urea) and abolished any response of KCC to changes in oxygen tension. Finally, although protein kinases have been implicated in regulation of phosphatidylserine exposure, WNK463 had no effect. Findings indicate a predominant role for WNKs in control of KCC in sickle cells but an apparent absence of downstream involvement of SPAK/OSR1. A more complete understanding of the mechanisms will inform pathogenesis whilst manipulation of WNK activity represents a potential therapeutic approach.

Effect of adrenaline and blood gas conditions on red cell volume and intra-erythrocytic electrolytes in the carp, Cyprinus carpio

1988 ◽  
Vol 137 (1) ◽  
pp. 457-476 ◽  
Author(s):  
D. A. Fuchs ◽  
C. Albers
Keyword(s):  
Intracellular Ph ◽  
Distribution Ratio ◽  
Haemoglobin Concentration ◽  
Blood Gases ◽  
Red Cells ◽  
Cell Swelling ◽  
Red Cell ◽  
Hypercapnic Hypoxia

1. Carp, when subjected to air stress in vivo, developed a hypoxaemia associated with an acidosis due to elevation of PCO2 and arterial lactate content. Adrenaline and noradrenaline levels rose markedly, and the mean corpuscular haemoglobin concentration (MCHC in mmol Hb l-1 red blood cells) decreased, indicating swelling of the erythrocytes. 2. No effect of adrenaline could be observed in vitro after equilibration at normal pH and oxygenation level. 3. Simulation of air stress in vitro, by using hypercapnic hypoxia together with adrenaline, caused appreciable swelling of the red cells. The effect of adrenaline could be blocked by propranolol and was reversible when the blood gases were readjusted to normal values. Swelling due to hypercapnic hypoxia was not affected by propranolol. 4. At normal PO2, lowering pH by increasing PCO2 or adding HCl to the blood at PCO2 = 3.5 mmHg (1 mmHg = 133.3 Pa) both led to swelling of red cells; further swelling occurred when adrenaline was added. 5. At normal or even elevated intracellular pH, hypoxia led to red cell swelling; further swelling occurred when adrenaline was added. 6. The swelling of red cells under hypoxic and/or acidotic conditions (induced by either added HCl or increased PCO2) was associated with an increase in the intracellular K+, Na+ and Cl- levels, together with an increase in the Donnan distribution ratio of Cl-, rCl. delta K+:delta Na+ was 4:1. The swelling associated with the addition of adrenaline was also accompanied by an increase in K+, Na+, Cl- and rCl. In this case the ratio delta K+:delta Na+ was 1:2.4. 7. There was a correlation between the Donnan ratio, rCl, and the change in MCHC, even in those cases where extracellular and intracellular pH were above normal. In addition to pH and oxygen saturation, rCl should therefore be considered as a possible triggering factor for the action of adrenaline. 8. In addition to the increased catecholamine levels, stress during sampling may be inferred if low values of MCHC and high values of intraerythrocytic chloride and a high chloride distribution ratio, rCl, are found in whole blood.

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