scholarly journals Myostatin levels in skeletal muscle of hibernating ground squirrels

2011 ◽  
Vol 214 (15) ◽  
pp. 2522-2527 ◽  
Author(s):  
N. E. Brooks ◽  
K. H. Myburgh ◽  
K. B. Storey
Keyword(s):  
Skeletal Muscle ◽  
Ground Squirrels

scholarly journals Nitrogen recycling buffers against ammonia toxicity from skeletal muscle breakdown in hibernating arctic ground squirrels

2020 ◽  
Vol 2 (12) ◽  
pp. 1459-1471
Author(s):  
Sarah A. Rice ◽  
Gabriella A. M. Ten Have ◽  
Julie A. Reisz ◽  
Sarah Gehrke ◽  
Davide Stefanoni ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Ground Squirrels ◽  
Ammonia Toxicity ◽  
Nitrogen Recycling ◽  
Muscle Breakdown ◽  
Arctic Ground Squirrels

Regulation of UCP1 and UCP3 in arctic ground squirrels and relation with mitochondrial proton leak

2006 ◽  
Vol 101 (1) ◽  
pp. 339-347 ◽  
Author(s):  
Jamie L. Barger ◽  
Brian M. Barnes ◽  
Bert B. Boyer
Keyword(s):  
Skeletal Muscle ◽  
Ground Squirrels ◽  
Proton Leak ◽  
Mrna Levels ◽  
Nonesterified Fatty Acids ◽  
Protein Levels ◽  
Bat Mitochondria ◽  
Brown Adipose ◽  
Arctic Ground Squirrels ◽  
Ucp3 Expression

Uncoupling protein (UCP) 1 (UCP1) catalyzes a proton leak in brown adipose tissue (BAT) mitochondria that results in nonshivering thermogenesis (NST), but the extent to which UCP homologs mediate NST in other tissues is controversial. To clarify the role of UCP3 in mediating NST in a hibernating species, we measured Ucp3 expression in skeletal muscle of arctic ground squirrels in one of three activity states (not hibernating, not hibernating and fasted for 48 h, or hibernating) and housed at 5°C or −10°C. We then compared Ucp3 mRNA levels in skeletal muscle with Ucp1 mRNA and UCP1 protein levels in BAT in the same animals. Ucp1 mRNA and UCP1 protein levels were increased on cold exposure and decreased with fasting, with the highest UCP1 levels in thermogenic hibernators. In contrast, Ucp3 mRNA levels were not affected by temperature but were increased 10-fold during fasting and >3-fold during hibernation. UCP3 protein levels were increased nearly fivefold in skeletal muscle mitochondria isolated from fasted squirrels compared with nonhibernators, but proton leak kinetics in the presence of BSA were unchanged. Proton leak in BAT mitochondria also did not differ between fed and fasted animals but did show classical inhibition by the purine nucleotide GDP. Levels of nonesterified fatty acids were highest during hibernation, and tissue temperatures during hibernation were related to Ucp1, but not Ucp3, expression. Taken together, these results do not support a role for UCP3 as a physiologically relevant mediator of NST in muscle.

scholarly journals Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48884 ◽  
Author(s):  
Eva Andres-Mateos ◽  
Rebeca Mejias ◽  
Arshia Soleimani ◽  
Brian M. Lin ◽  
Tyesha N. Burks ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Ground Squirrels ◽  
Skeletal Muscle Regeneration

scholarly journals Analysis of microRNA expression during the torpor-arousal cycle of a mammalian hibernator, the 13-lined ground squirrel

2016 ◽  
Vol 48 (6) ◽  
pp. 388-396 ◽  
Author(s):  
Cheng-Wei Wu ◽  
Kyle K. Biggar ◽  
Bryan E. Luu ◽  
Kama E. Szereszewski ◽  
Kenneth B. Storey
Keyword(s):  
Skeletal Muscle ◽  
Ground Squirrel ◽  
Muscle Development ◽  
Expression Profiles ◽  
Ground Squirrels ◽  
Microrna Expression ◽  
Metabolic Reprogramming ◽  
Skeletal Muscle Atrophy ◽  
Tissue Specific ◽  
Go Annotation

Hibernation is a highly regulated stress response that is utilized by some mammals to survive harsh winter conditions and involves a complex metabolic reprogramming at the cellular level to maintain tissue protections at low temperature. In this study, we profiled the expression of 117 conserved microRNAs in the heart, muscle, and liver of the 13-lined ground squirrel ( Ictidomys tridecemlineatus) across four stages of the torpor-arousal cycle (euthermia, early torpor, late torpor, and interbout arousal) by real-time PCR. We found significant differential regulation of numerous microRNAs that were both tissue specific and torpor stage specific. Among the most significant regulated microRNAs was miR-208b, a positive regulator of muscle development that was found to be upregulated by fivefold in the heart during late torpor (13-fold during arousal), while decreased by 3.7-fold in the skeletal muscle, implicating a potential regulatory role in the development of cardiac hypertrophy and skeletal muscle atrophy in the ground squirrels during torpor. In addition, the insulin resistance marker miR-181a was upregulated by 5.7-fold in the liver during early torpor, which supports previous suggestions of hyperinsulinemia in hibernators during the early stages of the hibernation cycle. Although microRNA expression profiles were largely unique between the three tissues, GO annotation analysis revealed that the putative targets of upregulated microRNAs tend to enrich toward suppression of progrowth-related processes in all three tissues. These findings implicate microRNAs in the regulation of both tissue-specific processes and general suppression of cell growth during hibernation.

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