scholarly journals Changes in the synthesis, distribution and sulphation of glycosaminoglycans of cultured human skin fibroblasts upon ascorbate feeding

1983 ◽  
Vol 64 (1) ◽  
pp. 245-254
Author(s):  
M. Edward ◽  
R.F. Oliver
Keyword(s):  
Ascorbic Acid ◽  
Cell Density ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Collagen Production ◽  
Sulphated Glycosaminoglycans ◽  
Cell Fractions

The effect of ascorbic acid on the synthesis, distribution and sulphation of glycosaminoglycans by human skin fibroblasts has been examined. Medium was supplemented with ascorbate over several days, and cultures incubated with [3H]glucosamine and Na2(35)SO4 for 48 h, followed by analysis of the glycosaminoglycans in the medium, in collagenase and trypsin extracts, and in cell fractions. Ascorbate feeding resulted in a reduction in hyaluronate synthesis, which was the main 3H-labelled component and was distributed mainly in the medium fractions. Sulphated glycosaminoglycans showed a reduction in incorporation of 3H label, but increased sulphation following ascorbate feeding. In control cultures 53% of 3H-labelled sulphated glycosaminoglycans and 63% of 35S-labelled glycosaminoglycans were present in the medium fraction, while in ascorbate-fed cultures, 41% of 3H label and 38% 35S label were incorporated into medium-sulphated glycosaminoglycans. Ascorbate also caused an increase in cell density and in collagen production and deposition.

The effects of ascorbic acid on collagen synthesis by cultured human skin fibroblasts

1991 ◽  
Vol 19 (1) ◽  
pp. 48S-48S ◽  
Author(s):  
SHONA M.L. ANDERSON ◽  
W.H. IRWIN McLEAN ◽  
ROBERT J. ELLIOTT
Keyword(s):  
Ascorbic Acid ◽  
Human Skin ◽  
Collagen Synthesis ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Specific association of iduronic acid-rich dermatan sulphate with the extracellular matrix of human skin fibroblasts cultured on collagen gels

1983 ◽  
Vol 215 (1) ◽  
pp. 107-116 ◽  
Author(s):  
J T Gallagher ◽  
N Gasiunas ◽  
S L Schor
Keyword(s):  
Human Skin ◽  
Heparan Sulphate ◽  
Surface Membrane ◽  
Skin Fibroblasts ◽  
Collagen Gels ◽  
Human Skin Fibroblasts ◽  
Dermatan Sulphate ◽  
Cellular Functions ◽  
Iduronic Acid ◽  
Sulphated Glycosaminoglycans

Human skin fibroblasts cultured on collagen gels produced two dermatan sulphate species, one, enriched in iduronic acid residues, that bound specifically to the collagenous fibres of the gel, the other, enriched in glucuronic acid, that accumulated in the culture medium. Collagen-binding and collagen-non-binding dermatan sulphates were also produced by cells grown on plastic surfaces, but in these cultures each constituent was released into the growth medium. Net synthesis of dermatan sulphate was 3-fold higher in cells maintained on collagen gels. In contrast, heparan sulphate synthesis was not influenced by the nature of the culture surface. The concentration of heparan sulphate in surface-membrane extracts was similar for cells grown on plastic and on collagen gels, but cells cultured on collagen showed a notable increase in the content of surface-membrane dermatan sulphate. The patterns of synthesis and distribution of sulphated glycosaminoglycans observed in skin fibroblasts maintained on collagen gels may reflect differentiated cellular functions.

Royal Jelly Protects Against Ultraviolet B–Induced Photoaging in Human Skin Fibroblasts via Enhancing Collagen Production

2011 ◽  
Vol 14 (9) ◽  
pp. 899-906 ◽  
Author(s):  
Hye Min Park ◽  
Eunson Hwang ◽  
Kwang Gill Lee ◽  
Sang-Mi Han ◽  
Yunhi Cho ◽  
...  
Keyword(s):  
Human Skin ◽  
Royal Jelly ◽  
Ultraviolet B ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Collagen Production
Sign in / Sign up

Export Citation Format

Share Document