The effects of ascorbic acid on collagen synthesis by cultured human skin fibroblasts

1991 ◽  
Vol 19 (1) ◽  
pp. 48S-48S ◽  
Author(s):  
SHONA M.L. ANDERSON ◽  
W.H. IRWIN McLEAN ◽  
ROBERT J. ELLIOTT
Keyword(s):  
Ascorbic Acid ◽  
Human Skin ◽  
Collagen Synthesis ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Collagen Synthesis in Cultured Human Skin Fibroblasts: Effect of Ascorbic Acid and Its Analogs

1983 ◽  
Vol 81 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Saood Murad ◽  
Shingo Tajima ◽  
Gail R. Johnson ◽  
Arunthathy Sivarajah ◽  
Sheldon R. Pinnell
Keyword(s):  
Ascorbic Acid ◽  
Human Skin ◽  
Collagen Synthesis ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

Collagen Synthesis in Growing Human Skin Fibroblasts

Pathobiology ◽  
1986 ◽  
Vol 54 (4) ◽  
pp. 177-182
Author(s):  
Daniel Kirchhofer ◽  
Christoph A. Reinhardt ◽  
Gerhard Zbinden
Keyword(s):  
Human Skin ◽  
Collagen Synthesis ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Changes in the synthesis, distribution and sulphation of glycosaminoglycans of cultured human skin fibroblasts upon ascorbate feeding

1983 ◽  
Vol 64 (1) ◽  
pp. 245-254
Author(s):  
M. Edward ◽  
R.F. Oliver
Keyword(s):  
Ascorbic Acid ◽  
Cell Density ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Collagen Production ◽  
Sulphated Glycosaminoglycans ◽  
Cell Fractions

The effect of ascorbic acid on the synthesis, distribution and sulphation of glycosaminoglycans by human skin fibroblasts has been examined. Medium was supplemented with ascorbate over several days, and cultures incubated with [3H]glucosamine and Na2(35)SO4 for 48 h, followed by analysis of the glycosaminoglycans in the medium, in collagenase and trypsin extracts, and in cell fractions. Ascorbate feeding resulted in a reduction in hyaluronate synthesis, which was the main 3H-labelled component and was distributed mainly in the medium fractions. Sulphated glycosaminoglycans showed a reduction in incorporation of 3H label, but increased sulphation following ascorbate feeding. In control cultures 53% of 3H-labelled sulphated glycosaminoglycans and 63% of 35S-labelled glycosaminoglycans were present in the medium fraction, while in ascorbate-fed cultures, 41% of 3H label and 38% 35S label were incorporated into medium-sulphated glycosaminoglycans. Ascorbate also caused an increase in cell density and in collagen production and deposition.

Alkaline phosphatase activation and collagen synthesis in human skin fibroblasts in culture

1973 ◽  
Vol 80 (1) ◽  
pp. 170-174 ◽  
Author(s):  
M.D. Waters ◽  
G.K. Summer ◽  
B.R. Switzer ◽  
R.D. Moore ◽  
D.H. Heitkamp
Keyword(s):  
Alkaline Phosphatase ◽  
Human Skin ◽  
Collagen Synthesis ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Phosphatase Activation
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