Fluorescent staining of fungal nuclei with a benzimidazol derivative

1978 ◽  
Vol 29 (1) ◽  
pp. 77-84
Author(s):  
P.A. Lemke ◽  
B. Kugleman ◽  
H. Morimoto ◽  
E.C. Jacobs ◽  
J. Ellison
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescence Microscopy ◽  
Filamentous Fungus ◽  
Ribonucleic Acid ◽  
Agaricus Bisporus ◽  
Deoxyribonucleic Acid ◽  
Rapid Quenching ◽  
Staining Procedure ◽  
Fluorescent Staining ◽  
Yeast Saccharomyces Cerevisiae

A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from the procedure for removal of ribonucleic acid. 33258 Hoechst has been evaluated as a nuclear stain with a yeast (Saccharomyces cerevisiae) and a filamentous fungus (Agaricus bisporus) and appears to penetrate easily vegetative cells and spores of both fungi.

scholarly journals PRION PROTEIN AND AMYLOID BETA PEPTIDE INTERACT IN THE YEAST SACCHAROMYCES CEREVISIAE

2012 ◽  
Vol 10 (1) ◽  
pp. 74-80
Author(s):  
Aleksander A. Rubel ◽  
Viktoria V. Korzhova ◽  
Alsu F. Saifitdinova ◽  
Kirill S. Antonez ◽  
Sergey G. Inge-Vechtomov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescence Microscopy ◽  
Prion Protein ◽  
Amyloid Beta ◽  
Living Cells ◽  
Yeast Cells ◽  
Amyloid Beta Peptide ◽  
Yeast Saccharomyces Cerevisiae ◽  
3D Microscopy ◽  
Beta Peptide

SUMMARY: The possibility of interaction between Prion Protein and amyloid beta peptide in living cells of yeast Saccharomyces cerevisiae have been investigated by fluorescence 3D microscopy. Using the FR ET technique, it was shown that amyloid beta peptide and PrP interact in yeast cells. In the future, the yeast model can be used for investigation of the fine mechanisms of this interaction by fluorescence microscopy.

The Spermatogenesis and Embryology of Litomosoides carinii and Dirofilaria immitis

1960 ◽  
Vol 34 (1-2) ◽  
pp. 3-12 ◽  
Author(s):  
Angela E. R. Taylor
Keyword(s):  
Fluorescence Microscopy ◽  
Ribonucleic Acid ◽  
Phase Contrast ◽  
Dirofilaria Immitis ◽  
Deoxyribonucleic Acid ◽  
Ultra Violet ◽  
Cellular Distribution ◽  
Green Fluorescence ◽  
Filarial Worm ◽  
First Time

1. Phase-contrast, ultra-violet and fluorescence microscopy have proved useful tools in the study of the embryology of filarial worms.2. Spermatogenesis in L. carinii has been described for the first time for any filarial worm.3. The spermatozoa of L. carinii and D. immitis are remarkable in that they contain well defined chromosomes.4. The development of the ovum to the mature microfilaria has been described for both L. carinii and D. immitis.5. Staining with acridine orange has demonstrated the cellular distribution of deoxyribonucleic acid (green fluorescence) and ribonucleic acid (red fluorescence) in both living and fixed embryos of each species.

Fluorescence study of lectinlike receptors involved in the flocculation of the yeast Saccharomyces cerevisiae

1992 ◽  
Vol 38 (5) ◽  
pp. 405-409 ◽  
Author(s):  
C. L. Masy ◽  
A. Henquinet ◽  
M. M. Mestdagh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescence Microscopy ◽  
Key Words ◽  
Fluorescent Probes ◽  
Homogeneous Distribution ◽  
Fluorescence Study ◽  
Yeast Saccharomyces Cerevisiae ◽  
Surface Receptors ◽  
Affinity Constants ◽  
Polar Distribution

Flocculation of some yeasts involves lectinlike receptors with two different patterns of inhibition by sugars: mannose sensitive (MS) and glucose-mannose sensitive (GMS). The visualization and quantification of these receptors were performed using neoglycoproteins fluorescent probes. Fluorescence microscopy showed a homogeneous distribution of surface receptors for the strain belonging to the MS group and a polar distribution for cells belonging to the GMS group. Affinity constants, estimated by fluorimetry, were shown to have different values (MS, 2.6 ± 0.7 × 105 M−1; GMS, 2 ± 1 × 106 M−1), but the number of sites was estimated to be smaller for strain NCYC 1195 which belongs to the GMS group than for strain NCYC 869 from the MS group (MS, 2.4 ± 0.2 × 107 sites/cell; GMS, 3.9 ± 0.8 × 106 sites/cell). Key words: flocculation, neoglycoproteins, Saccharomyces, lectinlike.

Sign in / Sign up

Export Citation Format

Share Document