Fluorescence study of lectinlike receptors involved in the flocculation of the yeast Saccharomyces cerevisiae

1992 ◽  
Vol 38 (5) ◽  
pp. 405-409 ◽  
Author(s):  
C. L. Masy ◽  
A. Henquinet ◽  
M. M. Mestdagh

Flocculation of some yeasts involves lectinlike receptors with two different patterns of inhibition by sugars: mannose sensitive (MS) and glucose-mannose sensitive (GMS). The visualization and quantification of these receptors were performed using neoglycoproteins fluorescent probes. Fluorescence microscopy showed a homogeneous distribution of surface receptors for the strain belonging to the MS group and a polar distribution for cells belonging to the GMS group. Affinity constants, estimated by fluorimetry, were shown to have different values (MS, 2.6 ± 0.7 × 105 M−1; GMS, 2 ± 1 × 106 M−1), but the number of sites was estimated to be smaller for strain NCYC 1195 which belongs to the GMS group than for strain NCYC 869 from the MS group (MS, 2.4 ± 0.2 × 107 sites/cell; GMS, 3.9 ± 0.8 × 106 sites/cell). Key words: flocculation, neoglycoproteins, Saccharomyces, lectinlike.

1995 ◽  
Vol 73 (S1) ◽  
pp. 369-371 ◽  
Author(s):  
Susan S. Brown

A number of myosins have been identified in yeast (Saccharomyces cerevisiae), an organism ideally suited to dissecting out their different functions. We have learned that a temperature-sensitive defect in one of these myosins (Myo2p) can be partially overcome by overexpression of a kinesin-like protein (Smy1p). This raises the possibility of the involvement of microtubules in the same function as Myo2p. However, we have been unable to demonstrate that this is the case, either using nocodazole to depolymerize microtubules or by altering the nucleotide-binding site of Smy1p. Key words: myosin, kinesin-related protein, cytoskeleton.


1992 ◽  
Vol 38 (9) ◽  
pp. 969-974 ◽  
Author(s):  
Eduardo V. Soares ◽  
José A. Teixeira ◽  
Manuel Mota

Interaction between nonflocculent and flocculent cells of Saccharomyces cerevisiae was studied. Adhesion experiments were done using three types of nonflocculent cells and a flocculent one. Two types of nonflocculent cells were obtained from the flocculent strain by changing environmental growth conditions. The integration of nonflocculent cells in the flocs was observed by two different methods: measurement of the sedimentation capacity of mixtures and microscopic observation of stained nonflocculent cells blended with flocculent cells. It was possible to verify that cell–cell interaction corresponds to a true stable binding and not to a simple entrapment inside the floe matrix. Key words: yeast, Saccharomyces cerevisiae, flocculation, adhesion.


1992 ◽  
Vol 38 (2) ◽  
pp. 149-152 ◽  
Author(s):  
J. Hašek ◽  
J. Jochová ◽  
P. Dráber ◽  
V. Viklický ◽  
E. Streiblová

Using the monoclonal antibody MA-01, which recognizes a 210-kDa protein in cell-free extracts, spindle and cytoplasmic microtubules were visualized in budding yeast, Saccharomyces cerevisiae. In additional, a spot-like staining was found beneath the plasma membrane, revealing in part correlation with F-actin distribution. This pattern was common for cells of all cell-cycle stages. The interaction of the protein recognized by MA-01 with microtubules was confirmed in the double labeling with a polyclonal antitubulin antibody and by the sensitivity of intranuclear structures stained by MA-01 to the microtubule disrupting drug nocodazole. Key words: immunoblotting, immunofluorescence, microtubule-interacting protein, Saccharomyces cerevisiae.


2012 ◽  
Vol 10 (1) ◽  
pp. 74-80
Author(s):  
Aleksander A. Rubel ◽  
Viktoria V. Korzhova ◽  
Alsu F. Saifitdinova ◽  
Kirill S. Antonez ◽  
Sergey G. Inge-Vechtomov ◽  
...  

SUMMARY: The possibility of interaction between Prion Protein and amyloid beta peptide in living cells of yeast Saccharomyces cerevisiae have been investigated by fluorescence 3D microscopy. Using the FR ET technique, it was shown that amyloid beta peptide and PrP interact in yeast cells. In the future, the yeast model can be used for investigation of the fine mechanisms of this interaction by fluorescence microscopy.


Author(s):  
Karelen Araujo ◽  
Ana Cáceres ◽  
Maria Berradre ◽  
Zulay Mármol ◽  
Johanna Raga ◽  
...  

  El sector vitivinícola mundial se encuentra inmerso en un importante proceso de actualización y renovación. En este contexto es interesante resaltar la actividad y la innovación de muchas bodegas que experimentan con nuevas variedades de uva, la utilización de uvas y levaduras autóctonas, así como con nuevas tecnologías para producir vinos más adaptados al gusto del consumidor. Todos estos cambios conllevan al desarrollo de nuevos productos con propiedades únicas que requieren de un estricto control de calidad. El objetivo del presente trabajo es determinar el perfil fisicoquímico de un vino blanco producido mediante fermentación alcohólica de cepas resultantes de la fusión intergénica de protoplastos de la levadura autóctona Saccharomyces cerevisiae SCVMLUZ 2008 y la levadura comercial Hanseniaspora guilliermondii CECT 11102 (Colección Española de Cultivos Tipo). Se realizaron vinificaciones en blanco con mosto de uva de la variedad Malvasía. Se realizaron los siguientes análisis físico-químicos como sólidos solubles, azúcares, pH, densidad relativa, acidez titulable y volátil, dióxido de azufre total, dióxido de azufre libre y etanol. La cinética de consumo de los azúcares fue más rápida en los bioprocesos realizados con S. cerevisiae y la levadura híbrida (SCHLUZ2014). Todas las levaduras de estudio consumieron glucosa a mayor velocidad que fructosa.  Los resultados obtenidos indican que el vino obtenido con la cepa híbrida cumple con los estándares establecidos por las Organizaciones Nacionales e Internacionales.   Palabras clave: vino blanco, levadura hibrida, consumo de azucares, características fisicoquímicas.   Abstract The world wine sector is immersed in an important process of updating and renewal. In this context, it is interesting to highlight the activity and innovation of many wineries experimenting with new grape varieties, the use of native grapes and yeasts, as well as new technologies to produce wines more adapted to the taste of the consumer. All these changes lead to the development of new products with unique properties that require a strict quality control. The objective of this work is to determine the physicochemical profile of white wine produced by alcoholic fermentation of strains resulting from the intergenic fusion of protoplasts of the indigenous yeast Saccharomyces cerevisiae SCVMLUZ 2008 and the commercial yeast Hanseniaspora guilliermondii CECT 11102 (Colección Española de Cultivos Tipo) White vinifications were made with grape must of the Malvasia variety. The following physical-chemical analyzes were carried out: soluble solids, sugars, pH, relative density, titratable and volatile acidity, total and free sulfur dioxide and ethanol. The kinetics of sugar consumption was faster in the bioprocesses performed with S. cerevisiae and the hybrid yeast (SCHLUZ2014). All the study yeasts consumed glucose at a faster rate than fructose. The results obtained indicate that the wine obtained with the hybrid strain complies with the standards established by the National and International Organizations.   Key words: Key words: white wine, hybrid yeast, consumption of sugars, physicochemical characteristics.


1978 ◽  
Vol 29 (1) ◽  
pp. 77-84
Author(s):  
P.A. Lemke ◽  
B. Kugleman ◽  
H. Morimoto ◽  
E.C. Jacobs ◽  
J. Ellison

A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from the procedure for removal of ribonucleic acid. 33258 Hoechst has been evaluated as a nuclear stain with a yeast (Saccharomyces cerevisiae) and a filamentous fungus (Agaricus bisporus) and appears to penetrate easily vegetative cells and spores of both fungi.


2016 ◽  
Vol 13 (1) ◽  
pp. 93
Author(s):  
Titin Yulinery ◽  
Ratih M.Dewi

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency


Tsitologiya ◽  
2018 ◽  
Vol 60 (7) ◽  
pp. 555-557 ◽  
Author(s):  
E. A. Alekseeva ◽  
◽  
T. A. Evstyukhina ◽  
V. T. Peshekhonov ◽  
V. G. Korolev ◽  
...  

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