scholarly journals Kinesin KIF18A is a novel PUM-regulated target promoting mitotic progression and survival of a human male germ cell line

2020 ◽  
Vol 133 (7) ◽  
pp. jcs240986 ◽  
Author(s):  
Maciej Jerzy Smialek ◽  
Bogna Kuczynska ◽  
Erkut Ilaslan ◽  
Damian Mikolaj Janecki ◽  
Marcin Piotr Sajek ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Male Germ Cell ◽  
Mitotic Progression ◽  
Germ Cell Line ◽  
Human Male

scholarly journals Human NANOS1 Represses Apoptosis by Downregulating Pro-Apoptotic Genes in the Male Germ Cell Line

2020 ◽  
Vol 21 (8) ◽  
pp. 3009
Author(s):  
Damian M. Janecki ◽  
Erkut Ilaslan ◽  
Maciej J. Smialek ◽  
Marcin P. Sajek ◽  
Maciej Kotecki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Germ Cell ◽  
Cell Line ◽  
Germ Cells ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Sequencing Analysis ◽  
Germ Cell Line ◽  
Apoptotic Genes

While two mouse NANOS paralogues, NANOS2 and NANOS3, are crucial for maintenance of germ cells by suppression of apoptosis, the mouse NANOS1 paralogue does not seem to regulate these processes. Previously, we described a human NANOS1 p.[(Pro34Thr);(Ser83del)] mutation associated with the absence of germ cells in seminiferous tubules of infertile patients, which might suggest an anti-apoptotic role of human NANOS1. In this study, we aimed to determine a potential influence of human NANOS1 on the maintenance of TCam-2 model germ cells by investigating proliferation, cell cycle, and apoptosis. Constructs encoding wild-type or mutated human NANOS1 were used for transfection of TCam-2 cells, in order to investigate the effect of NANOS1 on cell proliferation, which was studied using a colorimetric assay, as well as apoptosis and the cell cycle, which were measured by flow cytometry. RNA-Seq (RNA sequencing) analysis followed by RT-qPCR (reverse transcription and quantitative polymerase chain reaction) was conducted for identifying pro-apoptotic genes repressed by NANOS1. Here, we show that overexpression of NANOS1 downregulates apoptosis in TCam-2 cells. Moreover, we found that NANOS1 represses a set of pro-apoptotic genes at the mRNA level. We also found that the infertility-associated p.[(Pro34Thr);(Ser83del)] mutation causes NANOS1 to functionally switch from being anti-apoptotic to pro-apoptotic in the human male germ cell line. Thus, this report is the first to show an anti-apoptotic role of NANOS1 exerted by negative regulation of mRNAs of pro-apoptotic genes.

407 REGULATION OF HUMAN MALE GERM CELL DEATH BY MODULATORS OF ATP PRODUCTION

2005 ◽  
Vol 53 (1) ◽  
pp. S149.5-S149
Author(s):  
K. Erkkila ◽  
L. Dunkel ◽  
S. Kyttanen ◽  
R. S. Swerdloff
Keyword(s):  
Cell Death ◽  
Germ Cell ◽  
Male Germ Cell ◽  
Atp Production ◽  
Human Male

Effect of kinase-negativeEGFR gene on differentiation of embryonic germ cell line EG4

2001 ◽  
Vol 46 (16) ◽  
pp. 1364-1366 ◽  
Author(s):  
Xin Xu ◽  
Xiaoqian Cong ◽  
Shangquan Zhang ◽  
Yuanchang Yan
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Germ Cell Line

Abstract 3410: Regulation of pluripotency and lineage differentiation in human male germ cell tumors

2011 ◽  
Author(s):  
Nirmala Jagadish ◽  
Manjunath Kustagi ◽  
Geetu Mendiratta ◽  
Ritu Kushwaha ◽  
Mukesh Bansal ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Male Germ Cell ◽  
Lineage Differentiation ◽  
Human Male
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