Expression of peroxisomal proteins provides clear evidence for the presence of peroxisomes in the male germ cell line GC1spg

2003 ◽  
Vol 103 (3-4) ◽  
pp. 360-365 ◽  
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Male Germ Cell ◽  
Germ Cell Line

scholarly journals Human NANOS1 Represses Apoptosis by Downregulating Pro-Apoptotic Genes in the Male Germ Cell Line

2020 ◽  
Vol 21 (8) ◽  
pp. 3009
Author(s):  
Damian M. Janecki ◽  
Erkut Ilaslan ◽  
Maciej J. Smialek ◽  
Marcin P. Sajek ◽  
Maciej Kotecki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Germ Cell ◽  
Cell Line ◽  
Germ Cells ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Sequencing Analysis ◽  
Germ Cell Line ◽  
Apoptotic Genes

While two mouse NANOS paralogues, NANOS2 and NANOS3, are crucial for maintenance of germ cells by suppression of apoptosis, the mouse NANOS1 paralogue does not seem to regulate these processes. Previously, we described a human NANOS1 p.[(Pro34Thr);(Ser83del)] mutation associated with the absence of germ cells in seminiferous tubules of infertile patients, which might suggest an anti-apoptotic role of human NANOS1. In this study, we aimed to determine a potential influence of human NANOS1 on the maintenance of TCam-2 model germ cells by investigating proliferation, cell cycle, and apoptosis. Constructs encoding wild-type or mutated human NANOS1 were used for transfection of TCam-2 cells, in order to investigate the effect of NANOS1 on cell proliferation, which was studied using a colorimetric assay, as well as apoptosis and the cell cycle, which were measured by flow cytometry. RNA-Seq (RNA sequencing) analysis followed by RT-qPCR (reverse transcription and quantitative polymerase chain reaction) was conducted for identifying pro-apoptotic genes repressed by NANOS1. Here, we show that overexpression of NANOS1 downregulates apoptosis in TCam-2 cells. Moreover, we found that NANOS1 represses a set of pro-apoptotic genes at the mRNA level. We also found that the infertility-associated p.[(Pro34Thr);(Ser83del)] mutation causes NANOS1 to functionally switch from being anti-apoptotic to pro-apoptotic in the human male germ cell line. Thus, this report is the first to show an anti-apoptotic role of NANOS1 exerted by negative regulation of mRNAs of pro-apoptotic genes.

scholarly journals Kinesin KIF18A is a novel PUM-regulated target promoting mitotic progression and survival of a human male germ cell line

2020 ◽  
Vol 133 (7) ◽  
pp. jcs240986 ◽  
Author(s):  
Maciej Jerzy Smialek ◽  
Bogna Kuczynska ◽  
Erkut Ilaslan ◽  
Damian Mikolaj Janecki ◽  
Marcin Piotr Sajek ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Male Germ Cell ◽  
Mitotic Progression ◽  
Germ Cell Line ◽  
Human Male

Effect of kinase-negativeEGFR gene on differentiation of embryonic germ cell line EG4

2001 ◽  
Vol 46 (16) ◽  
pp. 1364-1366 ◽  
Author(s):  
Xin Xu ◽  
Xiaoqian Cong ◽  
Shangquan Zhang ◽  
Yuanchang Yan
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Germ Cell Line

scholarly journals Erythropoietin production by a human testicular germ cell line

Blood ◽  
1983 ◽  
Vol 62 (5) ◽  
pp. 1132-1134 ◽  
Author(s):  
JL Ascensao ◽  
F Gaylis ◽  
D Bronson ◽  
EE Fraley ◽  
ED Zanjani
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Continuous Cell Line ◽  
Human Testis ◽  
Testicular Germ Cell ◽  
Germ Cell Line ◽  
Erythropoietin Production ◽  
Unique Model

Abstract An established human testis germ cell line (1411-H) was found to produce significant amounts of erythropoietin (Ep), the primary regulator of erythropoiesis. Media conditioned by the 1411-H cells stimulated erythropoiesis both in vivo and in vitro. This activity was neutralized by anti-Ep. This continuous cell line provides a unique model for the study of the mechanisms of control of Ep biogenesis.

A trisomic germ cell line and precocious chromatid segregation leads to recurrent trisomy 21 conception

1999 ◽  
Vol 104 (1) ◽  
pp. 23-28 ◽  
Author(s):  
J. Cozzi ◽  
Clare M. Conn ◽  
Joyce Harper ◽  
Robert M. L. Winston ◽  
M. Rindl ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Line ◽  
Trisomy 21 ◽  
Germ Cell Line ◽  
Chromatid Segregation
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