scholarly journals Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes

2018 ◽  
Vol 131 (9) ◽  
pp. jcs204750 ◽  
Author(s):  
Peter Hoch-Kraft ◽  
Robin White ◽  
Stefan Tenzer ◽  
Eva-Maria Krämer-Albers ◽  
Jacqueline Trotter ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Rna Helicase ◽  
Dual Role ◽  
Post Transcriptional Regulation

scholarly journals Differential regulation of oligodendrocyte markers by glucocorticoids: post-transcriptional regulation of both proteolipid protein and myelin basic protein and transcriptional regulation of glycerol phosphate dehydrogenase

1989 ◽  
Vol 86 (17) ◽  
pp. 6807-6811 ◽  
Author(s):  
S Kumar ◽  
R Cole ◽  
F Chiappelli ◽  
J de Vellis
Keyword(s):  
Transcriptional Regulation ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Proteolipid Protein ◽  
Fold Increase ◽  
Mrna Levels ◽  
Glycerol Phosphate ◽  
Post Transcriptional Regulation ◽  
Glycerol Phosphate Dehydrogenase

During neonatal development glucocorticoids potentiate oligodendrocyte differentiation and myelinogenesis by regulating the expression of myelin basic protein, proteolipid protein, and glycerol phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8). The actual locus at which hydrocortisone exerts its developmental influence on glial physiology is, however, not well understood. Glycerol phosphate dehydrogenase is glucocorticoid-inducible in oligodendrocytes at all stages of development both in vivo and in vitro. In newborn rat cerebral cultures, between 9 and 15 days in vitro, a 2- to 3-fold increase in myelin basic protein and proteolipid protein mRNA levels occurs in oligodendrocytes within 12 hr of hydrocortisone treatment. Immunostaining demonstrates that this increase in mRNAs is followed by a 2- to 3-fold increase in the protein levels within 24 hr. In vitro transcription assays performed with oligodendrocyte nuclei show an 11-fold increase in the transcriptional activity of glycerol phosphate dehydrogenase in response to hydrocortisone but no increase in transcription of myelin basic protein or proteolipid protein. These results indicate that during early myelinogenesis, glucocorticoids influence the expression of key oligodendroglial markers by different processes: The expression of glycerol phosphate dehydrogenase is regulated at the transcriptional level, whereas the expression of myelin basic protein and proteolipid protein is modulated via a different, yet uncharacterized, mechanism involving post-transcriptional regulation.

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

scholarly journals Dual Role of the Histone Variant H2A.Z in Transcriptional Regulation of Stress-Response Genes

2017 ◽  
Vol 29 (4) ◽  
pp. 791-807 ◽  
Author(s):  
Weronika Sura ◽  
Michał Kabza ◽  
Wojciech M. Karlowski ◽  
Tomasz Bieluszewski ◽  
Marta Kus-Slowinska ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Stress Response ◽  
Dual Role ◽  
Histone Variant ◽  
Stress Response Genes

scholarly journals Dual Role of Insulin in Transcriptional Regulation of the Acute Phase Reactant Ceruloplasmin

2002 ◽  
Vol 277 (31) ◽  
pp. 27903-27911 ◽  
Author(s):  
Vasudevan Seshadri ◽  
Paul L. Fox ◽  
Chinmay K. Mukhopadhyay
Keyword(s):  
Transcriptional Regulation ◽  
Acute Phase ◽  
Acute Phase Reactant ◽  
Dual Role ◽  
Phase Reactant

Role of phosphorylation in conformational adaptability of bovine myelin basic protein

1990 ◽  
Vol 7 (1) ◽  
pp. 32-40 ◽  
Author(s):  
Gladys E. Deibler ◽  
Audrey L. Stone ◽  
Marian W. Kies
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein
Sign in / Sign up

Export Citation Format

Share Document