Ciliary granule plaques: membrane-intercalated particle aggregates associated with Ca2+-binding sites in paramecium

1975 ◽  
Vol 18 (2) ◽  
pp. 257-269
Author(s):  
H. Plattner
Keyword(s):  
Binding Sites ◽  
Mean Value ◽  
Direct Vision ◽  
Grid Pattern ◽  
Square Grid ◽  
Particle Aggregates ◽  
Bloodless Field ◽  
Individual Plaque ◽  
Basal Position

In Paramecium nine rectangular aggregates of membrane-intercalated particles surround the freeze-cleaved membrane of the ciliary base. These “ciliary granule plaques” occur independently of the “ciliary necklace” which is observed in a more basal position on some cilia. In each individual plaque the approximately 10 nm large granules are arranged in a square grid pattern: the granules of one plaque invariably form 3 vertical rows (number of horizontally arranged particles per row: Nh = 3) and 3–6 horizontal rows (mean value fos intrahepatic tributaries then can be approached under direct vision in a relatively bloodless field for safe, accurate liquation and/or reconstruction.

An Assessment of Radioimmunoassay Procedures for Determination of Anti-Acetylcholine Receptor Antibodies in the Sera of Patients with Myasthenia Gravis

1981 ◽  
Vol 18 (3) ◽  
pp. 146-152 ◽  
Author(s):  
B Carter ◽  
R Harrison ◽  
G G Lunt ◽  
H Morris ◽  
T Savage-Marengo ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Binding Sites ◽  
Mean Value ◽  
Normal Human ◽  
The Mean ◽  
Receptor Antibodies ◽  
Anti Acetylcholine

A reproducible radioimmunoassay procedure for the determination of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is described and examined in detail. The assay combines features of a number of methods previously outlined and allows repeat determinations of antibody titre in a given myasthenic serum sample with coefficient of variation 6%. The mean ± standard deviation for normal human serum anti-acetylcholine receptor antibodies was found by this procedure to be 0·024 ± 0·033 nmol/l α-bungarotoxin binding sites whereas the range for myasthenic patients was 0–139·14 nmol/l with a mean value of 7·55 nmol/l α-bungarotoxin binding sites.

Four-core fiber square grid pattern generator

2007 ◽  
Author(s):  
Libo Yuan ◽  
Shigang Zhao ◽  
Wang Xue
Keyword(s):  
Pattern Generator ◽  
Grid Pattern ◽  
Square Grid ◽  
Core Fiber

PROGESTERONE RECEPTORS IN THE RAT UTERUS: VARIATIONS IN CYTOSOL AND NUCLEI DURING THE OESTROUS CYCLE AND PREGNANCY

1978 ◽  
Vol 76 (1) ◽  
pp. 43-48 ◽  
Author(s):  
M. T. VU HAI ◽  
F. LOGEAT ◽  
E. MILGROM
Keyword(s):  
Binding Sites ◽  
Total Concentration ◽  
Progesterone Receptors ◽  
Mean Value ◽  
Oestrous Cycle ◽  
Small Peak ◽  
Low Concentrations ◽  
The Mean ◽  
High Level ◽  
Very High

SUMMARY The concentration of progesterone receptors in rat uterine cytosol and nuclei was measured during the oestrous cycle and pregnancy. The method used allowed the measurement of the total concentration of binding sites (unbound and hormone-bound). During the oestrous cycle, the concentration of receptors in the cytosol peaked at pro-oestrus, low concentrations were observed at oestrus and metoestrus, and an increase was seen at dioestrus. In the nuclei, maximum concentrations occurred at pro-oestrus and dioestrus. The number of receptors in the cytosol was very low during the first half of pregnancy, but the concentration increased progressively after day 15 to attain a very high level (about 26 000 binding sites/cell) on day 22. In the nuclei, the concentration of receptors was low at the beginning of pregnancy. On day 5 (day of implantation) there was a slight increase, which corresponded to a decrease in the number of cytosolic receptors and a small peak in the level of progesterone in the plasma. Maximum concentrations were attained during a 'plateau' period between days 9 and 15. Thereafter, there was a decrease in the concentration of nuclear receptors and on day 22, the mean value was very low; in some animals, probably on the verge of parturition, no receptors were detectable in the nuclei.

Study of the square grid pattern in dielectric barrier discharge by a CCD digital camera

2009 ◽  
Author(s):  
Lifang Dong ◽  
Shuai Wang ◽  
Han Yue ◽  
Hong Xiao ◽  
Yujie Yang ◽  
...  
Keyword(s):  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Digital Camera ◽  
Grid Pattern ◽  
Dielectric Barrier ◽  
Square Grid

Presence of oestrogen receptors in human blood mononuclear cells and thymocytes

1986 ◽  
Vol 112 (3) ◽  
pp. 409-414 ◽  
Author(s):  
J. J. A. M. Weusten ◽  
M. A. Blankenstein ◽  
F. H.J. Gmelig-Meyling ◽  
H.J. Schuurman ◽  
L. Kater ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Binding Sites ◽  
Blood Donors ◽  
Mononuclear Cells ◽  
Mean Value ◽  
Scatchard Analysis ◽  
Oestrogen Receptors ◽  
Progestin Receptor ◽  
Binding Data ◽  
Blood Mononuclear Cells

Abstract. We studied oestrogen binding sites in blood mononuclear cells from healthy blood donors, patients with leukaemia or systemic lupus erythematosus, and in thymocytes, using the dextran-coated charcoal assay and Scatchard analysis of binding data. Using 3H-labelled oestradiol as ligand, inaccurate results were obtained which could be related to the low amounts of binding sites. Using 125I-labelled ligand, saturable oestradiol binding sites could be demonstrated in low amount (mean value 2.1 fmol/mg of cytosolic protein) and high affinity (mean Kd value 0.26 nm; mean Ka value 3.85 × 109 m−1). The binding could be inhibited by unlabelled oestradiol but not with oestrone, dihydrotestosterone, cortisol and the progestin-receptor ligand Org 2058. We conclude that blood mononuclear cells and thymocytes contain true oestrogen receptors. This conclusion supports current hypotheses on the involvement of such receptors in oestrogen-mediated modulation of the immune system.

scholarly journals Container Spacing Increases Container Capture of overhead Irrigation as Application Efficiency Declines

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 606d-606
Author(s):  
Richard C. Beeson ◽  
Thomas H. Yeager
Keyword(s):  
Irrigation Water ◽  
Grid Pattern ◽  
Square Grid ◽  
Irrigation Application ◽  
Application Efficiency ◽  
Overhead Irrigation ◽  
Ligustrum Japonicum ◽  
Viburnum Odoratissimum ◽  
Production Area

Ligustrum japonicum, Rhododendron indica `Southern Charm' and Viburnum odoratissimum in 10-L containers were placed in a square grid pattern and overhead irrigated using impact sprinklers (30.3 L/min). Plants were irrigated with 12.5 mm with containers touching and, at 5 cm spacings, up to 50 cm between containers. Irrigation water reaching container surfaces (percent capture) increased for all species as container spacing increased. However, the increase in percent capture did not increase irrigation application efficiency because the percent of production area covered by the containers declined as spacing increased. Application efficiency declined with each increase in spacing to a low of 7%. The effects of intraand inter-canopy interference are discussed.

Antidepressant therapy is followed by normalization 0f serum albumin conformation in patients with melancholic depression

2017 ◽  
Vol 41 (S1) ◽  
pp. S327-S327
Author(s):  
T. Syrejshchikova ◽  
N. Smolina ◽  
M. Uzbekov ◽  
G. Dobretsov ◽  
V. Krjukov ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Conformational Changes ◽  
Binding Sites ◽  
Mean Value ◽  
Antidepressant Therapy ◽  
Control Group ◽  
Melancholic Depression ◽  
Days Of Therapy ◽  
Competing Interest ◽  
Albumin Molecule

ObjectivesDiscovery of biomarkers for evaluation of efficacy of psychopharmacotherapy is important task.AimTo study parameters characteristic for albumin binding sited in melancholic depression (MD) using fluorescent laser spectroscopy in range of 30–50 picoseconds.Methods22 patients with MD (dep) (F33.1 and 2) were investigated in dynamics of antidepressant therapy (venlafaxine: 75–150 mg/daily) for 30 days. Control group (con) consists of 54 volunteers. Decay of fluorescence amplitude (A) of fluorescent probe K-35 from serum albumin was measured using laser. Earlier, we revealed 3 binding sites in albumin with amplitudes A1, A2 and A3 with decay time of 1, 3 and 9 nanoseconds, respectively.ResultsThere was revealed significant decrease of amplitude A1dep, normalized on mean value of A1 for controls (A1dep/A1con), for patients with MD after treatment with venlafaxine. In this case, A1depvalues decreased and were equal to A1 values of controls (P < 0.01): A1dep/A1con before treatment–1.23 and after 30 days of therapy–0.97 relative units; for controls this value was–1.00 relative units. The same type of normalization was observed for amplitudes A2 and A3 of melancholic patients. There were revealed significant changes of A3/A1 ratio that points out on conformational changes of serum albumin molecule in dynamics of venlafaxine therapy.ConclusionWe have registered unidirectional changes in albumin molecule in patients with MD. Investigated parameters can serve as potential biomarkers for evaluation of efficacy of psychopharmacotherapy.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Conformation of Ribosomes from Escherichia Coli

1974 ◽  
Vol 32 ◽  
pp. 210-211
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Binding Sites ◽  
Ribosomal Proteins ◽  
Fluorescent Dyes ◽  
Protein Biosynthesis ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Dimensional Structure ◽  
Complete Understanding ◽  
And Function

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.

Electron Probe Analysis of Muscle

1982 ◽  
Vol 40 ◽  
pp. 398-401
Author(s):  
A. V. Somlyo ◽  
H. Shuman ◽  
A. P. Somlyo
Keyword(s):  
Skeletal Muscle ◽  
Smooth Muscle ◽  
Sarcoplasmic Reticulum ◽  
Resting State ◽  
Binding Sites ◽  
Electron Probe ◽  
Frog Skeletal Muscle ◽  
Electron Probe Analysis ◽  
Probe Analysis

Electron probe analysis of frozen dried cryosections of frog skeletal muscle, rabbit vascular smooth muscle and of isolated, hyperpermeab1 e rabbit cardiac myocytes has been used to determine the composition of the cytoplasm and organelles in the resting state as well as during contraction. The concentration of elements within the organelles reflects the permeabilities of the organelle membranes to the cytoplasmic ions as well as binding sites. The measurements of [Ca] in the sarcoplasmic reticulum (SR) and mitochondria at rest and during contraction, have direct bearing on their role as release and/or storage sites for Ca in situ.

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