Affinity chromatography of DNA-binding proteins from human, murine and man-mouse hybrid cell lines

1975 ◽  
Vol 18 (1) ◽  
pp. 41-65
Author(s):  
E. Jost ◽  
R. Lennox ◽  
H. Harris
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Proteins ◽  
Animal Species ◽  
Intact Cell ◽  
Hybrid Cell ◽  
Dna Binding Proteins ◽  
Electrophoretic Mobilities ◽  
Species Specific ◽  
Hybrid Cell Lines

About 60 proteins from human and murine cell lines were isolated by their ability to bind to different preparations of DNA. In the intact cell, the majority of these proteins are to be found in the cell nucleus. The electrophoretic mobilities of the DNA-binding proteins from human, murine and man-mouse hybrid cell lines were compared in two-dimensional acrylamide gels. Few, if any, species-specific differences were found. These observations suggest that the structures of the vast majority of the proteins that interact with DNA are conserved through evolution. A molecular basis is thus provided for the intracellular of hybrid cells derived from different animal species.

scholarly journals Human cancer cell lines growth inhibition by GTn oligodeoxyribonucleotides recognizing single-stranded DNA-binding proteins

1998 ◽  
Vol 252 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Bruna Scaggiante ◽  
Carla Morassutti ◽  
Barbara Dapas ◽  
Giuseppe Tolazzi ◽  
Franca Ustulin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Binding Proteins ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Dna Binding Proteins ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines

Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins

1988 ◽  
Vol 8 (11) ◽  
pp. 4877-4888
Author(s):  
J Philippe ◽  
D J Drucker ◽  
W Knepel ◽  
L Jepeal ◽  
Z Misulovin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Islet Cell ◽  
Binding Proteins ◽  
Islet Cells ◽  
Dna Binding Proteins ◽  
Alpha Cells ◽  
Promoter Element ◽  
Base Pairs ◽  
Specific Expression

The glucagon gene is expressed specifically in the alpha cells of the pancreatic islets. We show here that 300 base pairs of the 5'-flanking region of the rat glucagon gene, linked to a chloramphenicol acetyltransferase reporter plasmid transfected into islet cell lines of different hormone-producing phenotypes, directs transcription only in glucagon-producing islet cells. Deletional and linker-scanning mutations and DNase I footprinting assays identify three transcriptional control elements within these 300 base pairs. Two of these elements (G2 and G3) independently display enhancerlike functions on both homologous and heterologous promoters in glucagon (alpha) cells, but only on heterologous promoters in insulin- (beta) and somatostatin- (delta) expressing cells, and not in non-islet cells. The proximal promoter element (G1), characterized by low intrinsic transcriptional activity, is critical for specific expression of the glucagon gene in alpha cells. However, nuclear extracts prepared from all three islet cell phenotypes give similar protection to the three control elements of the glucagon 5'-flanking sequence. We conclude that these phenotypically distinct islet cell lines all contain regulatory DNA-binding proteins interacting with the three control elements of the glucagon gene, but that factors interacting with the glucagon promoter result in transcriptional activation only in alpha cells, to restrict glucagon gene expression to these cells. These observations suggest that interactions of nuclear proteins with cis-control elements are involved in the programmed developmental expression of the islet polypeptide hormone genes.

Human-mouse hybrid cell lines and susceptibility to species-specific viruses

1971 ◽  
Vol 77 (1) ◽  
pp. 117-119 ◽  
Author(s):  
R. Pollack ◽  
J. Salas ◽  
R. Wang ◽  
T. Kusano ◽  
H. Green
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Species Specific ◽  
Hybrid Cell Lines

scholarly journals Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins.

1988 ◽  
Vol 8 (11) ◽  
pp. 4877-4888 ◽  
Author(s):  
J Philippe ◽  
D J Drucker ◽  
W Knepel ◽  
L Jepeal ◽  
Z Misulovin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Islet Cell ◽  
Binding Proteins ◽  
Islet Cells ◽  
Dna Binding Proteins ◽  
Alpha Cells ◽  
Promoter Element ◽  
Base Pairs ◽  
Specific Expression

The glucagon gene is expressed specifically in the alpha cells of the pancreatic islets. We show here that 300 base pairs of the 5'-flanking region of the rat glucagon gene, linked to a chloramphenicol acetyltransferase reporter plasmid transfected into islet cell lines of different hormone-producing phenotypes, directs transcription only in glucagon-producing islet cells. Deletional and linker-scanning mutations and DNase I footprinting assays identify three transcriptional control elements within these 300 base pairs. Two of these elements (G2 and G3) independently display enhancerlike functions on both homologous and heterologous promoters in glucagon (alpha) cells, but only on heterologous promoters in insulin- (beta) and somatostatin- (delta) expressing cells, and not in non-islet cells. The proximal promoter element (G1), characterized by low intrinsic transcriptional activity, is critical for specific expression of the glucagon gene in alpha cells. However, nuclear extracts prepared from all three islet cell phenotypes give similar protection to the three control elements of the glucagon 5'-flanking sequence. We conclude that these phenotypically distinct islet cell lines all contain regulatory DNA-binding proteins interacting with the three control elements of the glucagon gene, but that factors interacting with the glucagon promoter result in transcriptional activation only in alpha cells, to restrict glucagon gene expression to these cells. These observations suggest that interactions of nuclear proteins with cis-control elements are involved in the programmed developmental expression of the islet polypeptide hormone genes.

Chromosome segregation from cell hybrids. V. Does segregation result from asynchronous centromere separation?

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Jennifer A. Marshall Graves ◽  
Paula A. Zelesco
Keyword(s):  
Cell Lines ◽  
Chromosome Segregation ◽  
Hybrid Cell ◽  
Human Chromosomes ◽  
Specific Difference ◽  
Cell Hybrids ◽  
G 11 ◽  
Species Specific ◽  
Separation Cell ◽  
Hybrid Cell Lines

Hamster–mouse and hamster–human hybrid cell lines were used to test the hypothesis that a species-specific difference in the timing of centromere separation is the basis for preferential chromosome segregation from interspecific cell hybrids. Colcemid-treated preparations were C-banded to differentiate hamster and mouse chromosomes or G-11 banded to differentiate hamster and human chromosomes. Metaphase spreads showing at least some centromere separation were photographed and the extent of separation, and the species of origin, was determined for each chromosome. There was no evidence that centromere separation of segregant chromosomes was consistently premature or delayed.Key words: chromosome segregation, centromere separation, cell hybrids.

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