Chromosome segregation from cell hybrids. V. Does segregation result from asynchronous centromere separation?

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Jennifer A. Marshall Graves ◽  
Paula A. Zelesco
Keyword(s):  
Cell Lines ◽  
Chromosome Segregation ◽  
Hybrid Cell ◽  
Human Chromosomes ◽  
Specific Difference ◽  
Cell Hybrids ◽  
G 11 ◽  
Species Specific ◽  
Separation Cell ◽  
Hybrid Cell Lines

Hamster–mouse and hamster–human hybrid cell lines were used to test the hypothesis that a species-specific difference in the timing of centromere separation is the basis for preferential chromosome segregation from interspecific cell hybrids. Colcemid-treated preparations were C-banded to differentiate hamster and mouse chromosomes or G-11 banded to differentiate hamster and human chromosomes. Metaphase spreads showing at least some centromere separation were photographed and the extent of separation, and the species of origin, was determined for each chromosome. There was no evidence that centromere separation of segregant chromosomes was consistently premature or delayed.Key words: chromosome segregation, centromere separation, cell hybrids.

Chromosome segregation from cell hybrids. IV. Movement and position of segregant set chromosomes in early-phase interspecific cell hybrids

1988 ◽  
Vol 89 (1) ◽  
pp. 49-56
Author(s):  
P.A. Zelesco ◽  
J.A. Graves
Keyword(s):  
Chromosome Segregation ◽  
Early Phase ◽  
Metaphase Plate ◽  
Central Position ◽  
Chromosome Loss ◽  
Human Chromosomes ◽  
Hybrid Cells ◽  
Multipolar Mitosis ◽  
Cell Hybrids ◽  
G 11

We searched for evidence of aberrant movement or position of segregant set chromosomes in C-banded and G-11-banded early-phase hamster-mouse and hamster-human cell hybrids that had been prepared with minimal disruption. No evidence was obtained for an increased frequency of multipolar mitosis, delayed or precocious metaphase congression or anaphase segregation, or for exclusion of chromosomes from the daughter nuclei. However, in hamster-human hybrids, segregant set (human) chromosomes were observed to assume a central position within a ring of hamster chromosomes on the metaphase plate. Such non-random positioning may imply that the centromeres of segregant chromosomes make aberrant, or simply less efficient, attachments to the spindle in hybrid cells. This aberrant position may perhaps result indirectly in chromosome loss by interfering with the normal processes of replication, repair or transcription.

Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells

1975 ◽  
Vol 17 (3) ◽  
pp. 307-325
Author(s):  
C.J. Marshall
Keyword(s):  
Cell Lines ◽  
Ribosomal Rna ◽  
Hybrid Cell ◽  
Chromosome Constitution ◽  
Rrna Synthesis ◽  
28S Rrna ◽  
Human Chromosomes ◽  
Hybrid Cells ◽  
Nucleolar Organizers ◽  
Hybrid Cell Lines

A study has been made of the ribosomal RNA and chromosome constitution of man-mouse hybrid cells. Previous work has shown that no human 28s rRNA is detectable in man-mouse synkaryons. In general human chromosomes are lost from such hybrids. With a recently developed method for distinguishing mouse from human chromosomes, an analysis of various man-mouse hybrid cell lines has been made. This indicates that not all the human chromosomes bearing nucleolar organizers are lost in the hybrid cells and such loss cannot alone explain the absence of human 28s rRNA. An examination of the 28s rRNA synthesized by heterokaryons formed from several different parent cells has revealed that both parental types of 28s rRNA are present in heterokaryons. The control of rRNA synthesis in hybrid cells is discussed.

Human Chromosomes in 18 Man-Mouse Somatic Hybrid Cell Lines Analysed by Quinacrine Fluorescence

1973 ◽  
Vol 12 (3) ◽  
pp. 809-830
Author(s):  
P. W. ALLDERDICE ◽  
O. J. MILLER ◽  
P. L. PEARSON ◽  
G. KLEIN ◽  
H. HARRIS
Keyword(s):  
Human Chromosome ◽  
Cell Lines ◽  
Somatic Hybrid ◽  
Hybrid Cell ◽  
Staining Technique ◽  
Centromeric Heterochromatin ◽  
Human Chromosomes ◽  
Hybrid Line ◽  
Somatic Hybrid Cell ◽  
Hybrid Cell Lines

Chromosome studies were done on 18 somatic hybrid cell lines produced by fusing cells of the mouse A 9 line with cells of the human Daudi lymphoblastoid line derived from a patient with Burkitt's lymphoma. The human chromosomes were identified by their quinacrine fluorescent banding patterns. In one hybrid line the human chromosomes were identified also by the centromeric heterochromatin staining technique. Every human chromosome was identified in one or more of the hybrid lines. Some lines were homogeneous in terms of their human chromosome content, while others were quite heterogeneous. Detailed analysis of the A 9 chromosomes in one hybrid line showed very few changes in comparison with the chromosome constitution of the average A 9 cell.

Human-mouse hybrid cell lines and susceptibility to species-specific viruses

1971 ◽  
Vol 77 (1) ◽  
pp. 117-119 ◽  
Author(s):  
R. Pollack ◽  
J. Salas ◽  
R. Wang ◽  
T. Kusano ◽  
H. Green
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Species Specific ◽  
Hybrid Cell Lines

Primed in situ (PRINS) Labelling with Alu and Satellite Primers for Rapid Characterization of Human Chromosomes in Hybrid Cell Lines

1997 ◽  
Vol 5 (5) ◽  
pp. 307-312 ◽  
Author(s):  
Philippe Coullin ◽  
Brigitte Andréo ◽  
Jean Paul Charlieu ◽  
Jean-Jacques Candelier ◽  
Franck Pellestor
Keyword(s):  
Cell Lines ◽  
Hybrid Cell ◽  
Human Chromosomes ◽  
Rapid Characterization ◽  
Hybrid Cell Lines

Affinity chromatography of DNA-binding proteins from human, murine and man-mouse hybrid cell lines

1975 ◽  
Vol 18 (1) ◽  
pp. 41-65
Author(s):  
E. Jost ◽  
R. Lennox ◽  
H. Harris
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Proteins ◽  
Animal Species ◽  
Intact Cell ◽  
Hybrid Cell ◽  
Dna Binding Proteins ◽  
Electrophoretic Mobilities ◽  
Species Specific ◽  
Hybrid Cell Lines

About 60 proteins from human and murine cell lines were isolated by their ability to bind to different preparations of DNA. In the intact cell, the majority of these proteins are to be found in the cell nucleus. The electrophoretic mobilities of the DNA-binding proteins from human, murine and man-mouse hybrid cell lines were compared in two-dimensional acrylamide gels. Few, if any, species-specific differences were found. These observations suggest that the structures of the vast majority of the proteins that interact with DNA are conserved through evolution. A molecular basis is thus provided for the intracellular of hybrid cells derived from different animal species.

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