The binding of the mucoprotein from gastric mucus to cells in tissue culture and the inhibition of cell adhesion

1975 ◽  
Vol 17 (3) ◽  
pp. 617-631
Author(s):  
A. Allen ◽  
S.M. Minnikin
Keyword(s):  
Tissue Culture ◽  
Cell Adhesion ◽  
Hela Cells ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Gastric Mucus ◽  
Gastric Mucous ◽  
Baby Hamster Kidney Cells

The mucoprotein, which is responsible for the formation of gastric mucous gel in pig, has been shown to bind equally well to suspensions of baby hamster kidney cells, polyoma-virus-transformed baby hamster kidney cells and HeLa cells. The binding of the mucoprotein to the cells is dependent on Ca 2

scholarly journals Adhesion of cells to polystyrene surfaces.

1983 ◽  
Vol 97 (5) ◽  
pp. 1500-1506 ◽  
Author(s):  
A S Curtis ◽  
J V Forrester ◽  
C McInnes ◽  
F Lawrie
Keyword(s):  
Cell Adhesion ◽  
Sulfuric Acid ◽  
High Surface ◽  
Attenuated Total Reflection ◽  
Hydroxyl Groups ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Surface Hydroxyl ◽  
Sulfur Containing ◽  
Baby Hamster Kidney Cells

The surface treatment of polystyrene, which is required to make polystyrene suitable for cell adhesion and spreading, was investigated. Examination of surfaces treated with sulfuric acid or various oxidizing agents using (a) x-ray photoelectron and attenuated total reflection spectroscopy and (b) measurement of surface carboxyl-, hydroxyl-, and sulfur-containing groups by various radiochemical methods showed that sulfuric acid produces an insignificant number of sulfonic acid groups on polystyrene. This technique together with various oxidation techniques that render surfaces suitable for cell culture generated high surface densities of hydroxyl groups. The importance of surface hydroxyl groups for the adhesion of baby hamster kidney cells or leukocytes was demonstrated by the inhibition of adhesion when these groups were blocked: blocking of carboxyl groups did not inhibit adhesion and may raise the adhesion of a surface. These results applied to cell adhesion in the presence and absence of serum. The relative unimportance of fibronectin for the adhesion and spreading of baby hamster kidney cells to hydroxyl-rich surfaces was concluded when cells spread on such surfaces after protein synthesis was inhibited with cycloheximide, fibronectin was removed by trypsinization, and trypsin activity was stopped with leupeptin.

scholarly journals Cell-surface mucosubstances from trypsin disaggregation of normal and virus-transformed lines of baby-hamster kidney cells (Short Communication)

1973 ◽  
Vol 134 (4) ◽  
pp. 1123-1126 ◽  
Author(s):  
S. Megan Minnikin ◽  
Adrian Allen
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Short Communication ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Baby Hamster Kidney Cells

Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.

scholarly journals Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin).

1980 ◽  
Vol 86 (1) ◽  
pp. 104-112 ◽  
Author(s):  
F Grinnell
Keyword(s):  
Tissue Culture ◽  
Divalent Cations ◽  
Cell Receptor ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Plasma Fibronectin ◽  
Bovine Albumin ◽  
Latex Beads ◽  
Cold Insoluble Globulin ◽  
Baby Hamster Kidney Cells

Studies were carried out on the interactions of uncharged latex beads (0.76 micrometer) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread onCIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4 degrees to 37 degrees C but the rate was lowest at 4 degrees C. At 37 degrees C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37 degrees C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

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