Cell-Surface Mucosubstances from Trypsin Disaggregation of Normal and Polyoma-Virus-Transformed Lines of Baby-Hamster Kidney Cells

1973 ◽  
Vol 1 (2) ◽  
pp. 448-450
Author(s):  
ADRIAN ALLEN ◽  
S. MEGAN MINNIKIN
Keyword(s):  
Cell Surface ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Baby Hamster Kidney Cells

scholarly journals Cell-surface mucosubstances from trypsin disaggregation of normal and virus-transformed lines of baby-hamster kidney cells (Short Communication)

1973 ◽  
Vol 134 (4) ◽  
pp. 1123-1126 ◽  
Author(s):  
S. Megan Minnikin ◽  
Adrian Allen
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Short Communication ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Baby Hamster Kidney Cells

Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.

Cell surface receptors for wheat germ agglutinin and limulin in baby hamster kidney cells and ricin resistant variants

Biochimie ◽  
1981 ◽  
Vol 63 (3) ◽  
pp. 169-175 ◽  
Author(s):  
Angèle Obrenovitch ◽  
Claude Sene ◽  
Annie Claude Roche ◽  
Michel Monsigny ◽  
Peter Visher ◽  
...  
Keyword(s):  
Cell Surface ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Cell Surface Receptors ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Surface Receptors ◽  
Baby Hamster Kidney Cells

The binding of the mucoprotein from gastric mucus to cells in tissue culture and the inhibition of cell adhesion

1975 ◽  
Vol 17 (3) ◽  
pp. 617-631
Author(s):  
A. Allen ◽  
S.M. Minnikin
Keyword(s):  
Tissue Culture ◽  
Cell Adhesion ◽  
Hela Cells ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Gastric Mucus ◽  
Gastric Mucous ◽  
Baby Hamster Kidney Cells

The mucoprotein, which is responsible for the formation of gastric mucous gel in pig, has been shown to bind equally well to suspensions of baby hamster kidney cells, polyoma-virus-transformed baby hamster kidney cells and HeLa cells. The binding of the mucoprotein to the cells is dependent on Ca 2

scholarly journals The distribution and mobility of anionic sites on the surfaces of baby hamster kidney cells.

1975 ◽  
Vol 66 (3) ◽  
pp. 470-479 ◽  
Author(s):  
F Grinnell ◽  
M Q Tobleman ◽  
C R Hackenbrock
Keyword(s):  
Cell Surface ◽  
Cell Body ◽  
Cytochalasin B ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Anionic Sites ◽  
Visual Probe ◽  
Entire Cell ◽  
Initial Binding ◽  
Baby Hamster Kidney Cells

The distribution and mobility of anionic sites on the surfaces of baby hamster kidney cells were studied by utilizing the multivalent ligand, polycationic ferritin, as a visual probe. Our observations revealed that anionic sites are distributed over the entire cell surface, with the highest density of sites being located on cell surface microextensions. Following the initial binding of polycationic ferritin to the surface of unfixed cells, the ligand-bound anionic sites redistributed by migrating from the surface of microextensions to the surface of the cell body. In 20 min, this migration resulted in a total clearing of anionic sites from the surface of microextensions concomitant with the formation of patches of anionic sites on the surface of the cell body. Polycationic ferritin-induced migration and patch formation of anionic sites was not prevented by 2,4-dinitrophenol, N-ethylmaleimide, colchicine, or cytochalasin B. However, the ligand-induced redistribution of cell surface anionic sites was prevented by prefixation of cells with glutaraldehyde.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

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