scholarly journals Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force microscopy

2005 ◽  
Vol 118 (18) ◽  
pp. 4199-4206 ◽  
Author(s):  
P.-H. Puech
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Adhesion ◽  
Adhesion Forces ◽  
Measuring Cell ◽  
Force Microscopy ◽  
Atomic Force

Nanoscale adhesion forces between the fungal pathogen Candida albicans and macrophages

2016 ◽  
Vol 1 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Sofiane El-Kirat-Chatel ◽  
Yves F. Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Candida Albicans ◽  
Immune Cells ◽  
Fungal Pathogen ◽  
Fungal Pathogens ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

We establish atomic force microscopy as a new nanoscopy platform for quantifying the forces between fungal pathogens and immune cells.

scholarly journals Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow

2020 ◽  
Vol 10 (13) ◽  
pp. 4560 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Tatyana O. Pleshakova ◽  
Ivan D. Shumov ◽  
Andrey F. Kozlov ◽  
Tatyana S. Romanova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Enzymatic Activity ◽  
Liquid Flow ◽  
Flow Section ◽  
Liquid Motion ◽  
Aggregation State ◽  
Measuring Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Solution Studies

The influence of liquid motion in flow-based systems on the aggregation state of an enzyme and on its enzymatic activity was studied, with horseradish peroxidase (HRP) as an example. Our experiments were carried out in a setup modeling the flow section of the biosensor communication with a measuring cell containing a protein solution. Studies were conducted for a biosensor measuring cell located along the axis of a spiral-moving liquid flow. The aggregation state of the protein was determined with an atomic force microscopy-based sensor (AFM sensor). It has been demonstrated that upon flowing of water through silicone biosensor communications, an increased aggregation of HRP protein was observed, but, at the same time, its enzymatic activity did not change. Our results obtained herein are useful in the development of models describing the influence of liquid flow in biosensor communications on the properties of enzymes and other proteins. This is particularly important for the development of serologic protein biosensors, which are beginning to be used for the early diagnosis of oncological diseases (such as brain cancer, prostate cancer, breast cancer etc.). The results obtained herein should also be taken into account when considering possible changes in hemodynamics due to increased protein aggregation.

scholarly journals A modular atomic force microscopy approach reveals a large range of hydrophobic adhesion forces among bacterial members of the leaf microbiota

2019 ◽  
Vol 13 (7) ◽  
pp. 1878-1882 ◽  
Author(s):  
Maximilian Mittelviefhaus ◽  
Daniel B. Müller ◽  
Tomaso Zambelli ◽  
Julia A. Vorholt
Keyword(s):  
Atomic Force Microscopy ◽  
Large Range ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Binding strength between cell adhesion proteoglycans measured by atomic force microscopy

Science ◽  
1995 ◽  
Vol 267 (5201) ◽  
pp. 1173-1175 ◽  
Author(s):  
U. Dammer ◽  
O. Popescu ◽  
P. Wagner ◽  
D. Anselmetti ◽  
H. Guntherodt ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Adhesion ◽  
Binding Strength ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Adhesion force mapping on wood by atomic force microscopy: influence of surface roughness and tip geometry

2016 ◽  
Vol 3 (10) ◽  
pp. 160248 ◽  
Author(s):  
X. Jin ◽  
B. Kasal
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Wood Surface ◽  
Adhesion Force ◽  
Data Interpretation ◽  
Natural Fibre ◽  
Force Distribution ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

This study attempts to address the interpretation of atomic force microscopy (AFM) adhesion force measurements conducted on the heterogeneous rough surface of wood and natural fibre materials. The influences of wood surface roughness, tip geometry and wear on the adhesion force distribution are examined by cyclic measurements conducted on wood surface under dry inert conditions. It was found that both the variation of tip and surface roughness of wood can widen the distribution of adhesion forces, which are essential for data interpretation. When a common Si AFM tip with nanometre size is used, the influence of tip wear can be significant. Therefore, control experiments should take the sequence of measurements into consideration, e.g. repeated experiments with used tip. In comparison, colloidal tips provide highly reproducible results. Similar average values but different distributions are shown for the adhesion measured on two major components of wood surface (cell wall and lumen). Evidence supports the hypothesis that the difference of the adhesion force distribution on these two locations was mainly induced by their surface roughness.

Adhesion Forces between LewisX Determinant Antigens as Measured by Atomic Force Microscopy

2001 ◽  
Vol 40 (16) ◽  
pp. 3052-3055 ◽  
Author(s):  
Christophe Tromas ◽  
Javier Rojo ◽  
Jesús M. de la Fuente ◽  
Africa G. Barrientos ◽  
Ricardo García ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy of DNA and bacteriophage in air, water and propanol: the role of adhesion forces

1993 ◽  
Vol 21 (5) ◽  
pp. 1117-1123 ◽  
Author(s):  
Y.L. Lyubchenko ◽  
P.I. Oden ◽  
D. Lampner ◽  
S.M. Lindsay ◽  
K.A. Dunker
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Challenges of Working with Active Pharmaceutical Ingredients to Measure Adhesion Forces by Atomic Force Microscopy

2011 ◽  
Vol 17 (S2) ◽  
pp. 1128-1129
Author(s):  
A Vogt ◽  
J Reddel
Keyword(s):  
Atomic Force Microscopy ◽  
Active Pharmaceutical Ingredients ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Pharmaceutical Ingredients ◽  
Atomic Force

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

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