scholarly journals Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation

2008 ◽  
Vol 121 (7) ◽  
pp. 1002-1013 ◽  
Author(s):  
M. Inada ◽  
A. Follenzi ◽  
K. Cheng ◽  
M. Surana ◽  
B. Joseph ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Liver ◽  
Liver Epithelial Cells

MicroRNA-191, regulated by HIF-2α, is involved in EMT and acquisition of a stem cell-like phenotype in arsenite-transformed human liver epithelial cells

2018 ◽  
Vol 48 ◽  
pp. 128-136 ◽  
Author(s):  
Chao Chen ◽  
Qianlei Yang ◽  
Dapeng Wang ◽  
Fei Luo ◽  
Xinlu Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Human Liver ◽  
Liver Epithelial Cells

scholarly journals Mechanisms and regulation of vitamin C uptake: studies of the hSVCT systems in human liver epithelial cells

2008 ◽  
Vol 295 (6) ◽  
pp. G1217-G1227 ◽  
Author(s):  
Jack C. Reidling ◽  
Veedamali S. Subramanian ◽  
Tamara Dahhan ◽  
Mohammed Sadat ◽  
Hamid M. Said
Keyword(s):  
Ascorbic Acid ◽  
Epithelial Cells ◽  
Vitamin C ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Active Form ◽  
Acid Uptake ◽  
Regulatory Pathways ◽  
Liver Epithelial Cells ◽  
Uptake Process

Humans use two sodium-ascorbate cotransporters (hSVCT1 and hSVCT2) for transporting the dietary essential micronutrient ascorbic acid, the reduced and active form of vitamin C. Although the human liver plays a pivotal role in regulating and maintaining vitamin C homeostasis, vitamin C transport physiology and regulation of the hSVCT systems in this organ have not been well defined. Thus, this research used a human hepatic cell line (HepG2), confirming certain results with primary human hepatocytes and determined the initial rate of ascorbic acid uptake to be Na+ gradient, pH dependent, and saturable as a function of concentration over low and high micromolar ranges. Additionally, hSVCT2 protein and mRNA are expressed at higher levels in HepG2 cells and native human liver, and the cloned hSVCT2 promoter has more activity in HepG2 cells. Results using short interfering RNA suggest that in HepG2 cells, decreasing hSVCT2 message levels reduces the overall ascorbic acid uptake process more than decreasing hSVCT1 message levels. Activation of PKC intracellular regulatory pathways caused a downregulation in ascorbic acid uptake not mediated by a single predicted PKC-specific amino acid phosphorylation site in hSVCT1 or hSVCT2. However, PKC activation causes internalization of hSVCT1 but not hSVCT2. Examination of other intracellular regulatory pathways on ascorbic acid uptake determined that regulation also potentially occurs by PKA, PTK, and Ca2+/calmodulin, but not by nitric oxide-dependent pathways. These studies are the first to determine the overall ascorbic acid uptake process and relative expression, regulation, and contribution of the hSVCT systems in human liver epithelial cells.

High Expression of Human CYP2C in Immortalized Human Liver Epithelial Cells

1999 ◽  
Vol 13 (4-5) ◽  
pp. 633-638 ◽  
Author(s):  
R. Bort ◽  
J.V. Castell ◽  
A. Pfeifer ◽  
M.J. Gómez-Lechón ◽  
K. Macé
Keyword(s):  
Epithelial Cells ◽  
Human Liver ◽  
High Expression ◽  
Liver Epithelial Cells
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