scholarly journals Gene expression profiles among murine strains segregate with distinct differences in the progression of radiation-induced lung disease

2017 ◽  
Vol 10 (4) ◽  
pp. 425-437 ◽  
Author(s):  
Isabel L. Jackson ◽  
Fitsum Baye ◽  
Chirayu P. Goswami ◽  
Barry P. Katz ◽  
Andrew Zodda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lung Disease ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced

Gene Expression Profiles for Radiation-induced Thyroid Cancer

2011 ◽  
Vol 23 (4) ◽  
pp. 282-288 ◽  
Author(s):  
C. Maenhaut ◽  
V. Detours ◽  
G. Dom ◽  
D. Handkiewicz-Junak ◽  
M. Oczko-Wojciechowska ◽  
...  
Keyword(s):  
Gene Expression ◽  
Thyroid Cancer ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced

scholarly journals Patients with systemic sclerosis-associated pulmonary arterial hypertension express a genomic signature distinct from patients with interstitial lung disease

2018 ◽  
Vol 3 (3) ◽  
pp. 242-248 ◽  
Author(s):  
Matthew Moll ◽  
Romy B Christmann ◽  
Yuqing Zhang ◽  
Michael L Whitfield ◽  
Yu Mei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Systemic Sclerosis ◽  
Pulmonary Arterial Hypertension ◽  
Interstitial Lung Disease ◽  
Arterial Hypertension ◽  
Lung Disease ◽  
Expression Profiles ◽  
Area Under The Curve ◽  
Gene Expression Profiles ◽  
Pulmonary Arterial

Objective: Pulmonary arterial hypertension and interstitial lung disease are major causes of mortality in systemic sclerosis. We used a previously identified microarray biomarker to determine whether systemic sclerosis-pulmonary arterial hypertension and systemic sclerosis-interstitial lung disease patients demonstrate distinct gene expression profiles. Methods: Peripheral blood mononuclear cells were collected from healthy controls ( n = 10), systemic sclerosis patients without pulmonary hypertension (systemic sclerosis-no pulmonary arterial hypertension, n = 39), and systemic sclerosis-pulmonary arterial hypertension patients ( n = 21; mean pulmonary arterial pressure ≥25, pulmonary capillary wedge pressure ≤15, and pulmonary vascular resistance ≥3 Wood units) diagnosed by right heart catheterization. Systemic sclerosis-interstitial lung disease patients were defined as those with evidence of fibrosis on chest computed tomography and significant restriction (forced vital capacity <70% predicted, n = 11). Systemic sclerosis-pulmonary arterial hypertension biomarker included 69 genes selected by unbiased statistical screening of three publicly available microarray studies. RNA levels were measured by NanoString Technologies. Gene expression levels that were significantly correlated with pulmonary arterial hypertension (multiple statistical measures) were chosen as inputs into a forward selection logistic regression model. Results: When interstitial lung disease patients were included ( n = 64), four genes (S100P, CD8B1, CCL2, and TIMP1) and male sex predicted pulmonary arterial hypertension with a high level of accuracy (area under the curve = 0.83). Without interstitial lung disease patients ( n = 53), two genes (THBS1 and CD8B1) and male sex predicted pulmonary arterial hypertension with a high level of accuracy (area under the curve = 0.80). When examining systemic sclerosis patients with borderline elevated pulmonary pressures (mean pulmonary arterial pressure = 21–24 mmHg), gene expression changes closely resembled the systemic sclerosis-pulmonary arterial hypertension group, except for THBS1. Conclusion: Systemic sclerosis-pulmonary arterial hypertension and systemic sclerosis-interstitial lung disease have similar but distinct gene expression profiles. Many gene expression changes occur early in the disease course, potentially allowing early detection. THBS1 appears to be an important mediator in the development of pulmonary arterial hypertension-predominant phenotype. Further prospective investigation is warranted.

scholarly journals Replicative and radiation-induced aging: a comparison of gene expression profiles

Aging ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 2378-2387 ◽  
Author(s):  
Alexander M. Aliper ◽  
Marine E. Bozdaganyan ◽  
Philipp S. Orekhov ◽  
Alex Zhavoronkov ◽  
Andreyan N. Osipov
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced

scholarly journals Gene Expression Profiles Reveal Extracellular Matrix and Inflammatory Signaling in Radiation-Induced Premature Differentiation of Human Fibroblast in vitro

2021 ◽  
Vol 9 ◽  
Author(s):  
Carsten Herskind ◽  
Carsten Sticht ◽  
Ahmad Sami ◽  
Frank A. Giordano ◽  
Frederik Wenz
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Human Skin ◽  
Pathway Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Inflammatory Reactions ◽  
Signaling Mechanisms ◽  
Radiation Induced

PurposeFibroblasts are considered to play a major role in the development of fibrotic reaction after radiotherapy and premature radiation-induced differentiation has been proposed as a cellular basis. The purpose was to relate gene expression profiles to radiation-induced phenotypic changes of human skin fibroblasts relevant for radiogenic fibrosis.Materials and MethodsExponentially growing or confluent human skin fibroblast strains were irradiated in vitro with 1–3 fractions of 4 Gy X-rays. The differentiated phenotype was detected by cytomorphological scoring and immunofluorescence microscopy. Microarray analysis was performed on Human Genome U133 plus2.0 microarrays (Affymetrix) with JMP Genomics software, and pathway analysis with Reactome R-package. The expression levels and kinetics of selected genes were validated with quantitative real-time PCR (qPCR) and Western blotting.ResultsIrradiation of exponentially growing fibroblast with 1 × 4 Gy resulted in phenotypic differentiation over a 5-day period. This was accompanied by downregulation of cell cycle-related genes and upregulation of collagen and other extracellular matrix (ECM)-related genes. Pathway analysis confirmed inactivation of proliferation and upregulation of ECM- and glycosaminoglycan (GAG)-related pathways. Furthermore, pathways related to inflammatory reactions were upregulated, and potential induction and signaling mechanisms were identified. Fractionated irradiation (3 × 4 Gy) of confluent cultures according to a previously published protocol for predicting the risk of fibrosis after radiotherapy showed similar downregulation but differences in upregulated genes and pathways.ConclusionGene expression profiles after irradiation of exponentially growing cells were related to radiation-induced differentiation and inflammatory reactions, and potential signaling mechanisms. Upregulated pathways by different irradiation protocols may reflect different aspects of the fibrogenic process thus providing a model system for further hypothesis-based studies of radiation-induced fibrogenesis.

Regulators and mediators of radiation-induced fibrosis: Gene expression profiles and a rationale for Smad3 inhibition

2010 ◽  
Vol 143 (4) ◽  
pp. 525-530 ◽  
Author(s):  
Judy W. Lee ◽  
Richard A. Zoumalan ◽  
Cristian D. Valenzuela ◽  
Phuong D. Nguyen ◽  
John P. Tutela ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced

Radiation-Induced Changes in Gene-Expression Profiles for the SCC VII Tumor Cells Grown In Vitro and In Vivo

2006 ◽  
Vol 8 (7-8) ◽  
pp. 1263-1272 ◽  
Author(s):  
John A. Cook ◽  
Eric Y. Chuang ◽  
Mong-Hsun Tsai ◽  
Debbie Coffin ◽  
William Degraff ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced ◽  
Induced Changes

1327: Gene Expression Profiles in Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 349-350
Author(s):  
Gaelle Fromont ◽  
Michel Vidaud ◽  
Alain Latil ◽  
Guy Vallancien ◽  
Pierre Validire ◽  
...  
Keyword(s):  
Gene Expression ◽  
Benign Prostatic Hyperplasia ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Prostatic Hyperplasia
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