scholarly journals Zebrafish etv7 regulates red blood cell development through the cholesterol synthesis pathway

2013 ◽  
Vol 7 (2) ◽  
pp. 265-270 ◽  
Author(s):  
A. M. Quintana ◽  
F. Picchione ◽  
R. I. Klein Geltink ◽  
M. R. Taylor ◽  
G. C. Grosveld
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cholesterol Synthesis ◽  
Cell Development

scholarly journals Mutations in the zebrafishhmgcs1gene reveal a novel function for isoprenoids during red blood cell development

2018 ◽  
Author(s):  
Jose A. Hernandez ◽  
Victoria L. Castro ◽  
Nayeli Reyes-Nava ◽  
Laura P. Montes ◽  
Anita M. Quintana
Keyword(s):  
Transcription Factor ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cholesterol Synthesis ◽  
Cell Development ◽  
Cholesterol Homeostasis ◽  
List Type ◽  
Key Points ◽  
Primitive Erythropoiesis ◽  
Gata1 Transcription Factor

AbstractErythropoiesis is the process by which new red blood cells (RBCs) are formed and defects in this process can lead to anemia or thalassemia. The GATA1 transcription factor is an established mediator of RBC development. However, the upstream mechanisms that regulate the expression ofGATA1are not completely characterized. Cholesterol is one potential upstream mediator ofGATA1expression because previously published studies suggest that defects in cholesterol synthesis disrupt RBC differentiation. Here we characterize RBC development in a zebrafish harboring a single missense mutation in thehmgcs1gene (Vu57 allele).hmgcs1encodes the first enzyme in the cholesterol synthesis pathway and mutation ofhmgcs1inhibits cholesterol synthesis. We analyzed the number of RBCs inhmgcs1mutants and their wildtype siblings. Mutation ofhmgcs1resulted in a decrease in the number of mature RBCs, which coincides with reducedgata1aexpression. We combined these experiments with pharmacological inhibition and confirmed that cholesterol and isoprenoid synthesis are essential for RBC differentiation, but thatgata1aexpression is isoprenoid dependent. Collectively, our results reveal two novel upstream regulators of RBC development and suggest that appropriate cholesterol homeostasis is critical for primitive erythropoiesis.Key PointsThe products of the cholesterol synthesis pathway regulate red blood cell development during primitive erythropoiesis.Isoprenoids regulate erythropoiesis by modulating the expression of the GATA1 transcription factor.

scholarly journals Mutations in the zebrafish hmgcs1 gene reveal a novel function for isoprenoids during red blood cell development

2019 ◽  
Vol 3 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Jose A. Hernandez ◽  
Victoria L. Castro ◽  
Nayeli Reyes-Nava ◽  
Laura P. Montes ◽  
Anita M. Quintana
Keyword(s):  
Transcription Factor ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cholesterol Synthesis ◽  
Cholesterol Homeostasis ◽  
Wild Type ◽  
Primitive Erythropoiesis ◽  
Gata1 Transcription Factor ◽  
Upstream Regulators

Abstract Erythropoiesis is the process by which new red blood cells (RBCs) are formed and defects in this process can lead to anemia or thalassemia. The GATA1 transcription factor is an established mediator of RBC development. However, the upstream mechanisms that regulate the expression of GATA1 are not completely characterized. Cholesterol is 1 potential upstream mediator of GATA1 expression because previously published studies suggest that defects in cholesterol synthesis disrupt RBC differentiation. Here we characterize RBC development in a zebrafish harboring a single missense mutation in the hmgcs1 gene (Vu57 allele). hmgcs1 encodes the first enzyme in the cholesterol synthesis pathway and mutation of hmgcs1 inhibits cholesterol synthesis. We analyzed the number of RBCs in hmgcs1 mutants and their wild-type siblings. Mutation of hmgcs1 resulted in a decrease in the number of mature RBCs, which coincides with reduced gata1a expression. We combined these experiments with pharmacological inhibition and confirmed that cholesterol and isoprenoid synthesis are essential for RBC differentiation, but that gata1a expression is isoprenoid dependent. Collectively, our results reveal 2 novel upstream regulators of RBC development and suggest that appropriate cholesterol homeostasis is critical for primitive erythropoiesis.

scholarly journals Csk-binding protein controls red blood cell development via regulation of Lyn tyrosine kinase activity

2017 ◽  
Vol 46 ◽  
pp. 70-82.e10
Author(s):  
Janice H.C. Plani-Lam ◽  
Neli S. Slavova-Azmanova ◽  
Nicole Kucera ◽  
Alison Louw ◽  
Jiulia Satiaputra ◽  
...  
Keyword(s):  
Blood Cell ◽  
Tyrosine Kinase ◽  
Red Blood Cell ◽  
Kinase Activity ◽  
Binding Protein ◽  
Cell Development ◽  
Tyrosine Kinase Activity ◽  
Lyn Tyrosine Kinase

scholarly journals The role of Friend of GATA in primitive red blood cell development

2009 ◽  
Vol 331 (2) ◽  
pp. 527
Author(s):  
Mizuho S. Mimoto ◽  
Jan L. Christian
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Development

scholarly journals A regulatory function of long non-coding RNAs in red blood cell development

2017 ◽  
Vol 63 (4) ◽  
Author(s):  
Klaudia Kulczyńska ◽  
Miroslawa Siatecka
Keyword(s):  
Gene Expression ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Regulation Of Gene Expression ◽  
Cell Development ◽  
Regulatory Function ◽  
Proliferation And Differentiation ◽  
Maturation Stages ◽  
Non Coding Rnas ◽  
In Cis

During recent years it has been discovered that long non-coding RNAs are important regulators in many biological processes. In this review, we summarize the role of lncRNA in erythropoiesis. LncRNA are crucial for regulation of gene expression during both proliferation and differentiation stages of red blood cell development. Many are regulated by erythroidspecific transcription factors and some are expressed in a developmental stage-specific manner. The majority of individually studied lncRNAs are involved in regulating the terminal maturation stages of red cell differentiation. Their regulatory function is accomplished by various mechanisms, including direct regulation in cis or trans by the lncRNA product or by the cis-localized presence of the lncRNA transcription itself. These add additional levels of regulation of gene expression during erythropoiesis.

The splice factor PTBP1 regulates hematopoietic stem cell function and red blood cell development

2016 ◽  
Vol 44 (9) ◽  
pp. S95-S96
Author(s):  
Matilda Rehn ◽  
Anne-Katrine Frank ◽  
Sachin Pundhir ◽  
Nicolas Rapin ◽  
Ying Ge ◽  
...  
Keyword(s):  
Stem Cell ◽  
Blood Cell ◽  
Hematopoietic Stem Cell ◽  
Red Blood Cell ◽  
Cell Function ◽  
Cell Development ◽  
Splice Factor ◽  
Hematopoietic Stem

Human mitochondrial ATP-binding cassette transporter ABCB10 is required for efficient red blood cell development

2011 ◽  
Vol 157 (1) ◽  
pp. 151-154 ◽  
Author(s):  
Leilei Tang ◽  
Saskia M. Bergevoet ◽  
Greet Bakker-Verweij ◽  
Cornelis L. Harteveld ◽  
Piero C. Giordano ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Development ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

scholarly journals Widespread and dynamic translational control of red blood cell development

Blood ◽  
2017 ◽  
Vol 129 (5) ◽  
pp. 619-629 ◽  
Author(s):  
Juan R. Alvarez-Dominguez ◽  
Xu Zhang ◽  
Wenqian Hu
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Translational Control ◽  
Erythroid Differentiation ◽  
Cell Development ◽  
Key Points ◽  
Terminal Erythroid Differentiation

Key Points Critical roles for dynamic translational control during terminal erythroid differentiation. RBM38 can regulate translation during terminal erythropoiesis.

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