Karyotype analysis of teratocarcinomas and embryoid bodies of C3H mice

Development ◽  
1977 ◽  
Vol 38 (1) ◽  
pp. 77-92
Author(s):  
S. A. Iles ◽  
E. P. Evans
Keyword(s):  
Karyotype Analysis ◽  
Normal Karyotype ◽  
Embryoid Bodies ◽  
Mouse Embryos ◽  
Chromosome 1 ◽  
Chromosome 11 ◽  
The Third ◽  
Wide Range ◽  
C3h Mice ◽  
Length Changes

Karyotype and capacity for differentiation were determined in four transplantable teratomas, and their embryoid bodies, derived from C3H mouse embryos. An apparently normal karyotype was retained by one tumour and one subline that were able to differentiate into a wide range of tissues, but some chromosomal alterations were found in the two tumours and one subline that showed almost identical restrictions in their capacity for differentiation. Trisomy for chromosome 11 was shared by all three restricted tumours; two of the tumours had similar length changes in the same two chromosome (1 and 14) while the third was generally trisomic for four other chromosomes.

KARYOTYPE ANALYSIS OF MULTIPLE TRANSLOCATION STOCKS OF BARLEY

1973 ◽  
Vol 15 (2) ◽  
pp. 267-273 ◽  
Author(s):  
N. A. Tuleen
Keyword(s):  
Karyotype Analysis ◽  
Normal Karyotype ◽  
Chromosome 1 ◽  
Chromosome 3

Karyotype analysis of multiple translocation stocks of barley in which the translocated chromosomes could be distinguished from each other and from the remaining pairs revealed that chromosome 1 is not the longest chromosome in the normal karyotype. Estimates of the lengths of chromosomes 2 and 3 and the position of the breakpoints in T2-5a and T3-7d were also obtained. The data indicate that the breakpoint of T3-7d is in the short arm of chromosome 3 and not in the long arm as previously reported.

The Colonial Documentary Film in South and South-East Asia

2017 ◽  
Keyword(s):  
East Asia ◽  
Subject Matter ◽  
Documentary Film ◽  
East Asian ◽  
South East Asia ◽  
Post Colonial ◽  
Historical Perspectives ◽  
The Third ◽  
Wide Range ◽  
Global Understanding

Writing from a wide range of historical perspectives, contributors to the anthology shed new light on historical, theoretical and empirical issues pertaining to the documentary film, in order to better comprehend the significant transformations of the form in colonial, late colonial and immediate post-colonial and postcolonial times in South and South-East Asia. In doing so, this anthology addresses an important gap in the global understanding of documentary discourses, practices, uses and styles. Based upon in-depth essays written by international authorities in the field and cutting-edge doctoral projects, this anthology is the first to encompass different periods, national contexts, subject matter and style in order to address important and also relatively little-known issues in colonial documentary film in the South and South-East Asian regions. This anthology is divided into three main thematic sections, each of which crosses national or geographical boundaries. The first section addresses issues of colonialism, late colonialism and independence. The second section looks at the use of the documentary film by missionaries and Christian evangelists, whilst the third explores the relation between documentary film, nationalism and representation.

Duoethnography: A Polytheoretical Approach to (Re)Storing, (Re)Storying the Meanings That One Gives

2020 ◽  
pp. 396-423
Author(s):  
John Joseph Norris ◽  
Richard D. Sawyer
Keyword(s):  
Social Justice ◽  
Critical Theory ◽  
Third Space ◽  
The Third ◽  
The Core ◽  
Wide Range ◽  
Core Elements ◽  
Feminist Inquiry

This chapter summarizes the advancement of duoethnography throughout its fifteen-year history, employing examples from a variety of topics in education and social justice to provide a wide range of approaches that one may take when conducting a duoethnography. A checklist articulates what its cofounders consider the core elements of duoethnographies, additional features that may or may not be employed and how some studies purporting to be duoethnographies may not be so. The chapter indicates connections between duoethnography and a number of methodological concepts including the third space, the problematics of representation, feminist inquiry, and critical theory using published examples by several duoethnographers.

DETERMINATION OF BREAKPOINT POSITIONS IN BARLEY TRANSLOCATIONS BY KARYOTYPE ANALYSIS OF PERMANENT RINGS OF SIX CHROMOSOMES

1974 ◽  
Vol 16 (3) ◽  
pp. 539-548 ◽  
Author(s):  
N. A. Tuleen ◽  
J. H. Gardenhire
Keyword(s):  
Karyotype Analysis ◽  
Chromosome 1 ◽  
Crossing Over ◽  
Differential Segment

Five T1-5 and 10 T1-6 barley translocations were crossed with the translocation T1-7f. Plants in which the T1-5 and T1-6 translocations had been combined with T1-7f due to crossing over in the differential segment were selected in the F2 generation. One of the chromosomes present in plants carrying the translocations in the combined form is made up of parts of the three chromosomes involved in the two translocations, and the segmental arrangement of this tripartite chromosome is determined by the position of the breakpoints in chromosome 1. The karyotypes of these stocks were analyzed and the breakpoints in seven of the translocations were assigned to the same arm and eight to the opposite arm of chromosome 1 relative to the position of the breakpoint in T1-7f.

scholarly journals Cloning and Mapping of a Putative Barley NADPH-Dependent HC-Toxin Reductase

1997 ◽  
Vol 10 (2) ◽  
pp. 234-239 ◽  
Author(s):  
F. Han ◽  
A. Kleinhofs ◽  
A. Kilian ◽  
S. E. Ullrich
Keyword(s):  
Plant Species ◽  
Chromosome 9 ◽  
Immature Embryos ◽  
Chromosome 1 ◽  
Grass Species ◽  
Cochliobolus Carbonum ◽  
Wide Range ◽  
Young Leaves ◽  
High Conservation ◽  
Hc Toxin

The NADPH-dependent HC-toxin reductase (HCTR), encoded by Hm1 in maize, inactivates HC-toxin produced by the fungus Cochliobolus carbonum, and thus confers resistance to the pathogen. The fact that C. carbonum only infects maize (Zea mays) and is the only species known to produce HC-toxin raises the question: What are the biological functions of HCTR in other plant species? An HCTR-like enzyme may function to detoxify toxins produced by pathogens which infect other plant species (R. B. Meeley, G. S. Johal, S. E. Briggs, and J. D. Walton, Plant Cell, 4:71–77, 1992). Hm1 homolog in rice (Y. Hihara, M. Umeda, C. Hara, Q. Liu, S. Aotsuka, K. Toriyama, and H. Uchimiya, unpublished) and HCTR activity in barley, wheat, oats and sorghum have been reported (R. B. Meeley and J. D. Walton, Plant Physiol. 97:1080–1086, 1993). To investigate the sequence conservation of Hm1 and HCTR in barley and the possible relationship of barley Hm1 homolog to the known disease resistance genes, we cloned and mapped a barley (Hordeum vulgare) Hm1-like gene. A putative full-length cDNA clone, Bhm1-18, was isolated from a cDNA library consisting of mRNA from young leaves, inflorescences, and immature embryos. This 1,297-bp clone encodes 363 amino acids which show great similarity (81.6%) with the amino acid sequence of HM1 in maize. Two loci were mapped to barley molecular marker linkage maps with Bhm1-18 as the probe; locus A (Bhm1A) on the long arm of chromosome 1, and locus B (Bhm1B) on the short arm of chromosome 1 which is syntenic to maize chromosome 9 containing the Hm2 locus. The Bhm1-18 probe hybridized strongly to a Southern blot of a wide range of grass species, indicating high conservation of HCTR at the DNA sequence level among grasses. The HCTR mRNA was detected in barley roots, leaves, inflorescences, and immature embryos. The conservation of the HCTR sequence, together with its expression in other plant species (R. B. Meeley and J. D. Walton, Plant Physiol. 97:1080–1086, 1993), suggests HCTR plays an important functional role in other plant species.

scholarly journals Why and How to Switch to Genomic Selection: Lessons From Plant and Animal Breeding Experience

2021 ◽  
Vol 12 ◽  
Author(s):  
◽  
Aline Fugeray-Scarbel ◽  
Catherine Bastien ◽  
Mathilde Dupont-Nivet ◽  
Stéphane Lemarié
Keyword(s):  
Genetic Diversity ◽  
Genomic Selection ◽  
Plant Species ◽  
Animal Species ◽  
Animal Breeding ◽  
Breeding Cycle ◽  
Breeding Programs ◽  
The Third ◽  
Wide Range ◽  
Selection For

The present study is a transversal analysis of the interest in genomic selection for plant and animal species. It focuses on the arguments that may convince breeders to switch to genomic selection. The arguments are classified into three different “bricks.” The first brick considers the addition of genotyping to improve the accuracy of the prediction of breeding values. The second consists of saving costs and/or shortening the breeding cycle by replacing all or a portion of the phenotyping effort with genotyping. The third concerns population management to improve the choice of parents to either optimize crossbreeding or maintain genetic diversity. We analyse the relevance of these different bricks for a wide range of animal and plant species and sought to explain the differences between species according to their biological specificities and the organization of breeding programs.

scholarly journals The Culture of the Sakas of Semirechye: Contemporary State and Current Issues of Study

2021 ◽  
Vol 20 (3) ◽  
pp. 22-35
Author(s):  
Ernar A. Estemesov
Keyword(s):  
Iron Age ◽  
Social Groups ◽  
Archaeological Sites ◽  
Radiocarbon Dates ◽  
Funeral Rites ◽  
The Third ◽  
Urgent Task ◽  
Wide Range ◽  
Burial Mounds ◽  
Second Category

Purpose. The article describes the history and analysis of the main issues in the study of archaeological sites of the Saka period in Semirechye. They are presented by three main types on this territory: burial and memorial complexes, settlements and hoards. The first type of monuments includes numerous burial mounds, where the elite burials of “royal” type and ordinary burials are located. Both social groups are combined by the unity of funeral rites, and the main differences are the complexity of architecture, memorial practices, and richness of burial equipment in the “royal” type burial mounds. The second category of monuments is presented by the settlements that are mostly small in size. The constructions like half dugouts were discovered on them, which gave a rich ceramic material. The third type of monuments of the Saka period in Semirechye includes numerous hoards of bronze items. Some of them are represented by the cult objects (sacrificial tables, lamps and cauldrons) that mark the places of worship. A significant percentage of the hoards contain items of weapons, horse equipment and household purposes and, apparently, serve as offerings to the spirits. However, despite the considerable progress in the study of the Saka monuments of the Semirechye Region, the main problem is their cultural attribution at this time. Some researchers suggest that the independent Saka archaeological culture was formed and developed on the territory of Semirechye in the Early Iron Age, while others believe that the Saka monuments of this region belong to the broader historical and cultural community that also covers the neighboring regions of Kyrgyzstan and Xinjiang. Results Another important issue in the study of the Saka sites of Semirechye is to clarify the chronology of burial and memorial complexes. Up to now, the significant database of radiocarbon dates has been accumulated, which allows us to consider the chronological positions of a wide range of monuments in a new way. It was of great importance to obtain such dates from several burials of Karatuma necropolis, which showed that it belonged to the Saka period, since burial monuments of this appearance are traditionally dated back to the Wusun period. Conclusion. The necessity of solution of these problems is an urgent task for further research of burial and settlement objects of the Saka period in this region.

scholarly journals Perancangan dan Analisis Perbandingan Implementasi OSPF pada Jaringan IPv4 dan IPv6

2014 ◽  
Vol 2 (1) ◽  
pp. 40
Author(s):  
PAULINE RAHMIATI ◽  
DWI ARYANTA ◽  
TAUFIQ AGUNG PRIYADI
Keyword(s):  
Network Design ◽  
Shortest Path ◽  
Routing Protocol ◽  
Convergence Time ◽  
The Third ◽  
Wide Range ◽  
Packet Tracer ◽  
Maximum Limit ◽  
Better Than

ABSTRAKOSPF (Open Shortest Path First) adalah suatu routing protokol bersifat terbuka dan didukung oleh berbagai perangkat network. IPv4 telah mencapai batas maksimum dalam jumlah alamat sehingga IPv6 merupakan solusi dalam hal tersebut. Seperti IPv4, IPv6 juga sudah mulai diimplementasikan untuk routing protokol OSPF, oleh karena itu pada penelitian ini akan dirancang suatu routing protokol OSPF IPv6 dan sebagai bahan perbandingan akan dibandingkan dengan OSPF IPv4. Software Cisco Packet Tracer 5.3 digunakan untuk mensimulasikan perancangan jaringan yang dibuat. Pada penelitian ini akan dibandingkan 2 buah jaringan berbasis routing protokol OSPF, yaitu OSPF untuk IPv4 dan IPv6. Skenario pertama dilakukan 100 kali dalam 5 kasus untuk mengetahui nilai delay OSPF IPv4 dan OSPF IPv6. Skenario kedua dilakukan pemutusan link dilakukan sebanyak 30 kali, hal yang dilihat dari pengujian ini adalah hasil trace route dari cost yang ada. Skenario ketiga dilakukan dengan mengamati waktu konvergensi dari OSPF IPv4 dan IPv6. Secara keseluruhan nilai delay OSPF IPv6 lebih kecil dibandingkan dengan OSPF IPv4 sebesar 3-6%, Trace route dan nilai cost pada OSPF IPv6 dan OSPF IPv4 sama tetapi nilai delay OSPF IPv6 lebih kecil sebesar 3-6% dan waktu konvergensi OSPF IPv4 bernilai sama dengan OSPF IPv6 yaitu 10 detik.Kata kunci: OSPF, IPv4, Ipv6, delay, konvergensiABSTRACTOSPF (Open Shortest Path First) is a routing protocol that opened and supported by a wide range of network devices. IPv4 has reached the maximum limit on the number of addresses that IPv6 are a solution in this case. Same as IPv4, IPv6 also has begun to be implemented for the OSPF routing protocol, therefore this study wiould design an IPv6 OSPF routing protocol and as a comparison will be compared to IPv4 OSPF. Cisco Packet Tracer 5.3 software was used to simulate the made network design. This research would compare two pieces of network-based routing protocol OSPF, OSPF for IPv4 and IPv6. The first scenario was done 100 times in of 5 cases to determine the value of delay OSPF OSPF IPv4 and IPv6. The second scenario was carried out link terminations 30 times, it was seen from the tests was the result of trace route from the existing cost. The third scenario was done by observed at the convergence time of OSPF IPv4 and IPv6. The overall delay value OSPF IPv6 better than IPv4 OSPF by 3-6%, the trace route and the OSPF cost value of IPv6 and IPv4 OSPF were same but delay OSPF IPv6 was better 3-6% and convergence time was the same as IPv4 OSPF OSPF IPv6 as 10 seconds.Keywords: OSPF, IPv4, IPv6, delay, convergence

Synthesis and third-order nonlinear optical properties of novel ethynyl-linked heteropentamer composed of four porphyrins and one pyrene

2009 ◽  
Vol 13 (02) ◽  
pp. 275-282 ◽  
Author(s):  
Ning Sheng ◽  
Jing Sun ◽  
Yongzhong Bian ◽  
Jianzhuang Jiang ◽  
Dong Xu
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Three Dimensional ◽  
Spectroscopic Methods ◽  
Third Order ◽  
Zinc Compounds ◽  
Nlo Properties ◽  
Metal Free ◽  
The Third ◽  
Wide Range

Novel heteropentameric porphyrins-pyrene arrays, in which four meso-tetraphenyl porphyrins are linked to the center unit of pyrene by four acetylenyl bonds, were designed and synthesized. The newly synthesized heteropentameric compounds have been characterized by a wide range of spectroscopic methods. The third-order nonlinear optical (NLO) properties of both the metal-free and zinc compounds of the three-dimensional arrays were investigated by Z-scan experiments, showing enhanced NLO properties compared with that of the porphyrin and pyrene monomers.

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