Regional assignments of the genes for fumarate hydratase and guanylate kinase on chromosome 1 and for lysosomal acid phosphatase and esterase A4 on chromosome 11

1976 ◽  
Vol 16 (1-5) ◽  
pp. 105-107 ◽  
Author(s):  
N. Busby ◽  
J. Courval ◽  
U. Francke
Keyword(s):  
Acid Phosphatase ◽  
Fumarate Hydratase ◽  
Chromosome 1 ◽  
Chromosome 11 ◽  
Guanylate Kinase ◽  
Lysosomal Acid

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

Prostatic Acid Phosphatase (PAP) Differentiated Ultracytochemically from Lysosomal Acid Phosphate in the Rat with a PAP/Specific Substrate, Phosphorylcholine

1975 ◽  
Vol 33 ◽  
pp. 450-451
Author(s):  
W. Allen Shannon ◽  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Phosphate Buffer ◽  
Prostatic Carcinoma ◽  
Prostatic Acid Phosphatase ◽  
Chemotherapeutic Agents ◽  
Specific Substrate ◽  
Acid Phosphate ◽  
Lysosomal Acid ◽  
Design And Synthesis ◽  
New Chemotherapeutic Agents

During the design and synthesis of new chemotherapeutic agents for prostatic carcinoma based on phosphorylated agents which might be enzyme-activated to cytotoxicity, phosphorylcholine, [(CH3)3+NCH2CH2OPO3Ca]Cl-, has been indicated to be a very specific substrate for prostatic acid phosphatase (PAP). This phenomenon has led to the development of specific histochemical and ultracytochemical methods for PAP using modifications of the Gomori lead method for acid phosphatase. Comparative histochemical results in prostate and kidney of the rat have been published earlier with phosphorylcholine (PC) and β-glycerophosphate (βGP). We now report the ultracytochemical results.Minced tissues were fixed in 3% glutaraldehyde-0.1 M phosphate buffered (pH 7.4) for 1.5 hr and rinsed overnight in several changes of 0.05 M phosphate buffer (pH 7.0) containing 7.5% sucrose. Tissues were incubated 30 min to 2 hr in Gomori acid phosphatase medium (2) containing 0.1 M substrate, either PC or βGP.

High Degree of Homology Between Primary Structure of Human Lysosomal Acid Phosphatase and Human Prostatic Acid Phosphatase

1989 ◽  
Vol 370 (1) ◽  
pp. 177-182 ◽  
Author(s):  
Christoph PETERS ◽  
Carola GEIER ◽  
Regina POHLMANN ◽  
Abdul WAHEED ◽  
Kurt VON FIGURA ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Primary Structure ◽  
Prostatic Acid Phosphatase ◽  
Lysosomal Acid ◽  
High Degree

scholarly journals Sequential processing of lysosomal acid phosphatase by a cytoplasmic thiol proteinase and a lysosomal aspartyl proteinase.

1989 ◽  
Vol 8 (11) ◽  
pp. 3215-3219 ◽  
Author(s):  
S. Gottschalk ◽  
A. Waheed ◽  
B. Schmidt ◽  
P. Laidler ◽  
K. von Figura
Keyword(s):  
Acid Phosphatase ◽  
Lysosomal Acid ◽  
Sequential Processing ◽  
Thiol Proteinase

Two Different Genetic Markers for High and Low Myopia

1992 ◽  
Vol 2 (4) ◽  
pp. 196-199 ◽  
Author(s):  
M.V. Olmedo ◽  
J.I. Muñoz ◽  
M.J. Rodriguez-Cid ◽  
A. Carracedo ◽  
F.J. Gomez-Ulla ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
High Myopia ◽  
Control Population ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Significant Difference ◽  
Hardy Weinberg Equilibrium ◽  
Group 2 ◽  
And Control ◽  
Group 1

In myopia patients, Rh and acid phosphatase were typed in two groups: group 1 consisted of 214 patients with low myopia (−6 D or less); group 2 of 124 patients with high myopia (more than −6 D). Statistical analysis of the markers showed a good Hardy-Weinberg equilibrium for both groups. In the Rh system there was a significant difference between group 1 and the control population (p < 0.05), but not between group 2 and control (p > 0.1). In the case of ACP there was a significant difference between group 2 and the control population (p < 0.05), but not between group 1 and control (p > 0.25). We conclude that the observed association between myopia and Rh system (chromosome 1) involves low myopia, while the association between myopia and acid phosphatase (chromosome 2) involves high myopia. Further DNA researche will lead to more specific results.

scholarly journals Purkinje Cell Compartmentation in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant Mouse (Nax - Naked-Ataxia Mutant Mouse)

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94327 ◽  
Author(s):  
Karen Bailey ◽  
Maryam Rahimi Balaei ◽  
Ashraf Mannan ◽  
Marc R. Del Bigio ◽  
Hassan Marzban
Keyword(s):  
Acid Phosphatase ◽  
Purkinje Cell ◽  
Mutant Mouse ◽  
Lysosomal Acid

scholarly journals Localization of lysosomal acid phosphatase mRNA in mouse tissues.

1992 ◽  
Vol 40 (9) ◽  
pp. 1275-1282 ◽  
Author(s):  
C Geier ◽  
J Kreysing ◽  
H Boettcher ◽  
R Pohlmann ◽  
K von Figura
Keyword(s):  
Acid Phosphatase ◽  
Mrna Level ◽  
Pyramidal Cells ◽  
Housekeeping Gene ◽  
Lysosomal Acid ◽  
Tissue Sections ◽  
Adult Mice ◽  
Mouse Tissues ◽  
Major Species

We studied the expression of lysosomal acid phosphatase (LAP) in mouse by hybridizing Northern blots and tissue sections with the mouse LAP cDNA. Three mRNA species of 2.3, 3.2 and 5.2 KB were identified, which differ in the length of their 3' untranslated region (UTR). The 3.2 KB mRNA is expressed in equal amounts in all tissues and represents the major species in most tissues, whereas the amounts of the 2.3 and 5.2 KB species differ. In situ hybridization of different tissues of adult mice showed a uniform expression of LAP, as expected for a housekeeping gene, except in testis and brain. In testis we found an increase in the LAP mRNA level in spermatocytes. By Northern blot analysis of young mouse testis, this increase could be attributed to late pachytene primary spermatocytes or secondary spermatocytes. In brain tissue the neurons were predominantly labeled, especially the Purkinje and pyramidal cells, whereas glial cells expressed only low amounts of LAP mRNA. Very high LAP expression was also found in the epithelial cells of the choroid plexus. Analysis of LAP expression during mouse embryonic development between Days 9.5 and 17.5 revealed a prominent expression relative to other tissues in the neural tube from Day 9.5 to Day 13.5.

scholarly journals Computational Mining and Genome Wide Distribution of Microsatellite in Fusarium oxysporum f. sp. lycopersici

2012 ◽  
Vol 4 (4) ◽  
pp. 127-131 ◽  
Author(s):  
Sudheer KUMAR ◽  
Deepak MAURYA ◽  
Shalini RAI ◽  
Prem Lal KASHYAP ◽  
Alok Kumar SRIVASTAVA
Keyword(s):  
Fusarium Oxysporum ◽  
Genomic Analysis ◽  
Wide Distribution ◽  
Chromosome 1 ◽  
Coding Region ◽  
Chromosome 11 ◽  
High Throughput Analysis ◽  
Genome Wide ◽  
Baseline Information ◽  
Simple Sequence

Simple sequence repeat (SSR) is currently the most preferred molecular marker system owing to their highly desirable properties viz., abundance, hyper-variability, and suitability for high-throughput analysis. Hence, in present study an attempt was made to mine and analyze microsatellite dynamics in whole genome of Fusarium oxysporum f. sp. lycopersici. The distribution pattern of different SSR motifs provides the evidence of greater accumulation of tetra-nucleotide (3837) repeats followed by tri-nucleotide (3367) repeats. Maximum frequency distribution in coding region was shown by mono-nucleotide SSR motifs (34.8%), where as minimum frequency is observed for penta-nucleotide SSR (0.87%). Highest relative abundance (1023 SSR/Mb) and density of SSRs (114.46 bp/Mb) were observed on chromosome 1, while least density of SSR motifs was recorded on chromosome 11 (7.40 bp/Mb) and 12 (7.41 bp/Mb), respectively. Maximum trinucleotide (34.24%) motifs code for glutamic acid (GAA) while GT/CT were the most frequent repeat of dinucleotide SSRs. Most common and highly repeated SSR motifs were identified as (A)64, (T)48, (GT)24, (GAA)31, (TTTC)24, (TTTCT)28 and (AACCAG)27. Overall, the generated information may serve as baseline information for developing SSR markers that could find applications in genomic analysis of F. oxysporum f. sp. lycopersici for better understanding of evolution, diversity analysis, population genetics, race identification and acquisition of new virulence.

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