Cell lines derived from late embryonic stages of Drosophila melanogaster

Development ◽  
1972 ◽  
Vol 27 (2) ◽  
pp. 353-365 ◽  
Author(s):  
Imogene Schneider
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Cell Lines ◽  
Imaginal Disc ◽  
Primary Cultures ◽  
First Line ◽  
The Third ◽  
Insect Tissue Culture ◽  
Disc Cells

The development of three cell lines initiated from the late embryonic stages of Drosophila melanogaster is described. The primary cultures consisted of trypsinized fragments from embryos 20–24 h old. The length of time between primary culture and subsequent subculture varied from 8 months for the first line to 3 weeks for the third. All three lines have been maintained in vitro for more than a year. The characteristics of each line are given and evidence is presented that at least one line is derived from imaginal disc cells. A few comments on insect tissue culture in general are also made.

Development and differentiation in vitro of Drosophila imaginal disc cells from dissociated early embryos

Development ◽  
1975 ◽  
Vol 33 (2) ◽  
pp. 487-498
Author(s):  
Andreas Dübendorfer ◽  
Glen Shields ◽  
James H. Sang
Keyword(s):  
Imaginal Disc ◽  
Larval Instar ◽  
Cell Types ◽  
The Third ◽  
Early Embryos ◽  
Disc Cells ◽  
Development And Differentiation ◽  
Pattern Specification

Embryos of Drosophila melanogaster, 6–8 h after oviposition, were dissociated and the cells cultured in vitro. Besides larval cell types, imaginal disc cells, assembled and growing in bloated monolayered vesicles, were obtained. The cells of these vesicles become competent to differentiate adult structures when treated with α-ecdysone or ecdysterone in vitro. Recognizable patterns of the adult fly are not formed though. If metamorphosis of imaginal cell vesicles from in vitro-cultures is induced in vivo by transplantation into host larvae of various ages within the third larval instar, recognizable patterns can differentiate provided the host larva does not metamorphose prior to 2 days after transplantation. The frequency of specific patterns in the implants can be increased by providing 9 days of culture in vivo (adult host flies) before metamorphosis. Passage through the third larval instar is not essential for these cells to produce identifiable patterns since culture in adult flies alone can achieve this. The quality of the differentiated pattern is not correlated with the extent of cell proliferation in the cultured tissues. The problem of pattern specification in vitro and in vivo is discussed.

In vitro cultivation of hemocytes of Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae)

1971 ◽  
Vol 49 (10) ◽  
pp. 1355-1358 ◽  
Author(s):  
S. S. Sohi
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Primary Cultures ◽  
Malacosoma Disstria ◽  
In Vitro Cultivation ◽  
Original Source ◽  
Insect Tissue Culture ◽  
Fetal Bovine

Prolonged culturing of the hemocytes of Malacosoma disstria has been accomplished using Grace's insect tissue culture medium supplemented with fetal bovine serum (5%) and Bombyx mori hemolymph (3%). The cultures started to grow after 3–6 months. These cells have now been in vitro for over 16 months, and have been subcultured 35 times. Three types of cells were present in primary cultures, but only one type, prohemocytes, persisted and grew after subculturing. The M. disstria larvae that were used as the original source of hemocytes were naturally infected with the microsporidian Glugea disstriae. The microsporidian also grew in [he cell cultures, and the cells are still infected.

The growth and differentiation in vitro of leg and wing imaginal disc cells from Drosophila melanogaster

Development ◽  
1988 ◽  
Vol 102 (4) ◽  
pp. 805-814 ◽  
Author(s):  
D.A. Currie ◽  
M.J. Milner ◽  
C.W. Evans
Keyword(s):  
Culture System ◽  
Imaginal Disc ◽  
Primary Cultures ◽  
In Vitro Culture System ◽  
Disc Cells ◽  
Wing Imaginal Discs ◽  
Cuticular Structures ◽  
Pupal Cuticle ◽  
Wing Cell

We have devised a new in vitro culture system in which cells from dissociated Drosophila leg and wing imaginal discs grow and differentiate. Primary cultures consist of epithelial and fibroblast-like cells, together with some lamellocyte-like cells. These cultures have given rise to continuously dividing leg and wing cell lines, in which epithelial, fibroblast-like, lamellocyte-like and distinct sickle-shaped cells are found. Vesicles composed of epithelial cells form in primary cultures and these differentiate imaginal cuticular structures under the influence of 20-hydroxyecdysone. Two types of cuticle-like material are formed spontaneously in established cultures. One type is present as thin, untanned sheets resembling apolysed pupal cuticle, while the other consists of thicker, tanned material similar to imaginal cuticle.

scholarly journals Primary Cultures and Cell Lines for In Vitro Modeling of the Human Adrenal Cortex

2021 ◽  
Vol 253 (4) ◽  
pp. 217-232
Author(s):  
Kazutaka Nanba ◽  
Amy R. Blinder ◽  
William E. Rainey
Keyword(s):  
Adrenal Cortex ◽  
Cell Lines ◽  
Primary Cultures

scholarly journals Interleukin-HP1, a T cell-derived hybridoma growth factor that supports the in vitro growth of murine plasmacytomas.

1987 ◽  
Vol 165 (3) ◽  
pp. 641-649 ◽  
Author(s):  
J Van Snick ◽  
A Vink ◽  
S Cayphas ◽  
C Uyttenhove
Keyword(s):  
T Cell ◽  
Growth Factor ◽  
B Cell ◽  
Cell Lines ◽  
Primary Cultures ◽  
Gel Filtration ◽  
Reversed Phase ◽  
Hybridoma Growth ◽  
Cell Supernatant

We have recently described the purification and NH2-terminal amino acid sequence of a T cell-derived hybridoma growth factor that was provisionally designated interleukin-HP1 (IL-HP1). Here we report that a T cell supernatant containing high titers of this hybridoma growth factor considerably facilitated the establishment of primary cultures of murine plasmacytomas. Most plasmacytoma cell lines derived from such cultures remained permanently dependent on IL-HP1-containing T cell supernatant for both survival and growth in vitro. These cell lines, however, retained their ability to form tumors in irradiated pristane-treated mice. Analytical fractionation of a T cell supernatant rich in IL-HP1 by either gel filtration, isoelectric focusing, or reversed-phase HPLC revealed the existence of only one plasmacytoma growth factor activity that strictly copurified with IL-HP1, strongly suggesting the identity of both factors. This conclusion was further supported by the finding that IL-HP1 purified to homogeneity supported the growth of both B cell hybridomas and plasmacytomas. For half-maximal growth, plasmacytomas, however, required a concentration of IL-HP1 of approximately 30 pM, which is approximately 200 times higher than that required by B cell hybridomas. A clear difference in the specificity of IL-HP1 and B cell stimulatory factor 1 (BSF-1) was demonstrated by the finding that IL-HP1-dependent plasmacytomas did not survive in the presence of BSF-1, whereas helper T cell lines that proliferated in the presence of BSF-1 failed to respond to IL-HP1.

Expression of Liver Functions in Imrnortalised Rot Hepotocyte Cell Lines

1994 ◽  
Vol 13 (6) ◽  
pp. 439-444 ◽  
Author(s):  
Caroline MacDonald ◽  
Maureen Vass ◽  
Brian Willett ◽  
Alexander Scott ◽  
Helen Grant
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Hepatic Function ◽  
Glutathione S Transferase ◽  
Early Passage ◽  
Toxicological Studies ◽  
Well Differentiated

The differentiated hepatic function of two rat liver cell lines, P9 and SV40RH1, immortalised by transfection with SV40 DNA has been investigated in terms of the glutathione synthesis, and the activities of γ-glutamyltransferase, glutathione-S-transferase and UDP-glucuronosyltransferase. SV40RH1 is a highly differentiated cell line at early passage, but the expression of some aspects of its differentiated phenotype is unstable and some functions are lost by passage 12-13. P9 is a less-well differentiated cell line, with relatively stable expression of functions between passages 4 and 13. In terms of differentiated function both cell lines represent a marked improvement over primary cultures of rat hepatocytes which de-differentiate rapidly within 24-48 h in culture. This retention of liver function in proliferating cell lines offers the opportunity to use such cells in in vitro toxicological studies.

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