The growth and differentiation in vitro of leg and wing imaginal disc cells from Drosophila melanogaster

Development ◽  
1988 ◽  
Vol 102 (4) ◽  
pp. 805-814 ◽  
Author(s):  
D.A. Currie ◽  
M.J. Milner ◽  
C.W. Evans
Keyword(s):  
Culture System ◽  
Imaginal Disc ◽  
Primary Cultures ◽  
In Vitro Culture System ◽  
Disc Cells ◽  
Wing Imaginal Discs ◽  
Cuticular Structures ◽  
Pupal Cuticle ◽  
Wing Cell

We have devised a new in vitro culture system in which cells from dissociated Drosophila leg and wing imaginal discs grow and differentiate. Primary cultures consist of epithelial and fibroblast-like cells, together with some lamellocyte-like cells. These cultures have given rise to continuously dividing leg and wing cell lines, in which epithelial, fibroblast-like, lamellocyte-like and distinct sickle-shaped cells are found. Vesicles composed of epithelial cells form in primary cultures and these differentiate imaginal cuticular structures under the influence of 20-hydroxyecdysone. Two types of cuticle-like material are formed spontaneously in established cultures. One type is present as thin, untanned sheets resembling apolysed pupal cuticle, while the other consists of thicker, tanned material similar to imaginal cuticle.

Cell lines derived from late embryonic stages of Drosophila melanogaster

Development ◽  
1972 ◽  
Vol 27 (2) ◽  
pp. 353-365 ◽  
Author(s):  
Imogene Schneider
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Cell Lines ◽  
Imaginal Disc ◽  
Primary Cultures ◽  
First Line ◽  
The Third ◽  
Insect Tissue Culture ◽  
Disc Cells

The development of three cell lines initiated from the late embryonic stages of Drosophila melanogaster is described. The primary cultures consisted of trypsinized fragments from embryos 20–24 h old. The length of time between primary culture and subsequent subculture varied from 8 months for the first line to 3 weeks for the third. All three lines have been maintained in vitro for more than a year. The characteristics of each line are given and evidence is presented that at least one line is derived from imaginal disc cells. A few comments on insect tissue culture in general are also made.

scholarly journals In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid

2014 ◽  
Vol 29 (4) ◽  
pp. 457-469 ◽  
Author(s):  
Federica Riva ◽  
Claudia Omes ◽  
Roberto Bassani ◽  
Rossella E Nappi ◽  
Giuliano Mazzini ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Progenitor Cells ◽  
Follicular Fluid ◽  
Culture System ◽  
Mesenchymal Progenitor Cells ◽  
In Vitro Culture System ◽  
Ovarian Follicular Fluid

scholarly journals Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro

2011 ◽  
Vol 40 (1) ◽  
pp. 124-128
Author(s):  
Sabine Wohlres-Viana ◽  
Mariana Cortes Boite ◽  
João Henrique Moreira Viana ◽  
Marco Antonio Machado ◽  
Luiz Sérgio de Almeida Camargo
Keyword(s):  
Culture System ◽  
Rna Extraction ◽  
Endogenous Control ◽  
Bovine Embryos ◽  
Relative Expression ◽  
Gapdh Gene ◽  
Expression Of Genes ◽  
In Vitro Culture System

The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase β2 genes, however, it affects expression of Na/K-ATPase α1.

First description of an in vitro culture system for Eimeria ovinoidalis macromeront formation in primary host endothelial cells

2016 ◽  
Vol 65 (5) ◽  
pp. 516-519 ◽  
Author(s):  
Tessa Carrau ◽  
Liliana Machado Ribeiro Silva ◽  
David Pérez ◽  
Rocio Ruiz de Ybáñez ◽  
Anja Taubert ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Primary Host ◽  
In Vitro Culture System

Development and differentiation in vitro of Drosophila imaginal disc cells from dissociated early embryos

Development ◽  
1975 ◽  
Vol 33 (2) ◽  
pp. 487-498
Author(s):  
Andreas Dübendorfer ◽  
Glen Shields ◽  
James H. Sang
Keyword(s):  
Imaginal Disc ◽  
Larval Instar ◽  
Cell Types ◽  
The Third ◽  
Early Embryos ◽  
Disc Cells ◽  
Development And Differentiation ◽  
Pattern Specification

Embryos of Drosophila melanogaster, 6–8 h after oviposition, were dissociated and the cells cultured in vitro. Besides larval cell types, imaginal disc cells, assembled and growing in bloated monolayered vesicles, were obtained. The cells of these vesicles become competent to differentiate adult structures when treated with α-ecdysone or ecdysterone in vitro. Recognizable patterns of the adult fly are not formed though. If metamorphosis of imaginal cell vesicles from in vitro-cultures is induced in vivo by transplantation into host larvae of various ages within the third larval instar, recognizable patterns can differentiate provided the host larva does not metamorphose prior to 2 days after transplantation. The frequency of specific patterns in the implants can be increased by providing 9 days of culture in vivo (adult host flies) before metamorphosis. Passage through the third larval instar is not essential for these cells to produce identifiable patterns since culture in adult flies alone can achieve this. The quality of the differentiated pattern is not correlated with the extent of cell proliferation in the cultured tissues. The problem of pattern specification in vitro and in vivo is discussed.

In vitro imaginal disc development and moulting hormone

Development ◽  
1980 ◽  
Vol 57 (1) ◽  
pp. 107-118
Author(s):  
Christiane Guillermet ◽  
Paul Mandaron
Keyword(s):  
Cell Differentiation ◽  
Imaginal Disc ◽  
Subsequent Development ◽  
Hormone Concentration ◽  
Drosophila Larvae ◽  
Wing Discs ◽  
Moulting Hormone ◽  
Pupal Cuticle ◽  
Different Levels

Imaginal leg and wing discs obtained from late-third-instar Drosophila larvae were cultured in vitro in various concentrations of ecdysterone ranging from 10−10 to 10−5 M inorder to test the effect of hormone concentration on evagination and cell differentiation. At the optimal concentration of 8 × 10−8 M discs evaginated normally, secreted the pupal cuticle, underwent apolysis, differentiated imaginal structures and secreted the imaginal cuticle. At suboptimal concentrations (10−8 M and less), evagination was incomplete in a variable proportion of appendages. Morphogenetic movements were limited to the earlier ones; so that appendages did not emerge from the peripodial sac. Subsequent development, whenever it occurred, took place inside the peripodial sac. This particular type of ‘endoevagination’ was only obtained with sub-optimal hormone concentration. At supra-optimal concentrations (10−6 M and more), evagination was always complete but further differentiation was inhibited. These results show that endoevagination is strictly related to insufficient supply ofhormone and that morphogenesis and cell differentiation in imaginal discs are two independent phenomena, which respond to different levels of hormone stimulation.

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1623-1636 ◽  
Author(s):  
Chu-Chih Shih ◽  
Mickey C.-T. Hu ◽  
Jun Hu ◽  
Jeffrey Medeiros ◽  
Stephen J. Forman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Ex Vivo ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Culture System

Abstract We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD34+thy-1+ cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD34+ thy-1+ cells on AC6.21 stroma, a murine bone marrow–derived stromal cell line, caused expansion of cells with CD34+ thy-1+ phenotype. Addition of other cytokines, including interleukin-3 (IL-3), IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, to LIF in the cultures caused a 150-fold expansion of cells retaining the CD34+ thy-1+ phenotype. The ex vivo–expanded CD34+ thy-1+ cells gave rise to multilineage differentiation, including myeloid, T, and B cells, when transplanted into SCID-hu mice. Both murine LIF (cannot bind to human LIF receptor) and human LIF caused expansion of human CD34+ thy-1+ cells in vitro, suggesting action through the murine stroma. Furthermore, another human HSC candidate, CD34+ CD38− cells, shows a similar pattern of proliferative response. This suggests thatex vivo expansion of transplantable human stem cells under this in vitro culture system is a general phenomenon and not just specific for CD34+ thy-1+ cells.

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