Retraction: Btbd7 is essential for region-specific epithelial cell dynamics and branching morphogenesis in vivo

Development ◽  
2020 ◽  
Vol 147 (15) ◽  
pp. dev195560
Author(s):  
William P. Daley ◽  
Kazue Matsumoto ◽  
Andrew D. Doyle ◽  
Shaohe Wang ◽  
Brian J. DuChez ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Branching Morphogenesis ◽  
Cell Dynamics

scholarly journals Region-specific epithelial cell dynamics during branching morphogenesis

2013 ◽  
Vol 242 (9) ◽  
pp. C1-C1 ◽  
Author(s):  
Jeff C. Hsu ◽  
Hyun Koo ◽  
Jill S. Harunaga ◽  
Kazue Matsumoto ◽  
Andrew D. Doyle ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Branching Morphogenesis ◽  
Cell Dynamics

The role of laminin-5 and its receptors in mammary epithelial cell branching morphogenesis

1997 ◽  
Vol 110 (1) ◽  
pp. 55-63 ◽  
Author(s):  
S. Stahl ◽  
S. Weitzman ◽  
J.C. Jones
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Branching Morphogenesis ◽  
Mammary Epithelial ◽  
Breast Epithelial Cell ◽  
Epithelial Tissue ◽  
Laminin 5 ◽  
Normal Mammary Epithelial

In vivo, normal mammary epithelial cells utilize hemidesmosome attachment devices to adhere to stroma. However, analyses of a potential role for hemidesmosomes and their components in mammary epithelial tissue morphogenesis have never been attempted. MCF-10A cells are a spontaneously immortalized line derived from mammary epithelium and possess a number of characteristics of normal mammary epithelial cells including expression of hemidesmosomal associated proteins such as the two bullous pemphigoid antigens, alpha 6 beta 4 integrin and its ligand laminin-5. More importantly, MCF-10A cells readily assemble mature hemidesmosomes when plated onto uncoated substrates. When maintained on matrigel, like their normal breast epithelial cell counterparts, MCF-10A cells undergo a branching morphogenesis and assemble hemidesmosomes at sites of cell-matrigel interaction. Function blocking antibodies specific for human laminin-5 and the alpha subunits of its two known receptors (alpha 3 beta 1 and alpha 6 beta 4 integrin) not only inhibit hemidesmosome assembly by MCF-10A cells but also impede branching morphogenesis induced by matrigel. Our results imply that the hemidesmosome, in particular those subunits comprising its laminin-5/integrin ‘backbone’, play an important role in morphogenetic events. We discuss these results in light of recent evidence that hemidesmosomes are sites involved in signal transduction.

scholarly journals Region-specific epithelial cell dynamics during branching morphogenesis

2013 ◽  
Vol 242 (9) ◽  
pp. 1066-1077 ◽  
Author(s):  
Jeff C. Hsu ◽  
Hyun Koo ◽  
Jill S. Harunaga ◽  
Kazue Matsumoto ◽  
Andrew D. Doyle ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Branching Morphogenesis ◽  
Cell Dynamics

scholarly journals Determination of the curvature of epithelial cell mass by mesenchyme in branching morphogenesis of mouse salivary gland

Development ◽  
1983 ◽  
Vol 73 (1) ◽  
pp. 221-232
Author(s):  
Hiroyuki Nogawa
Keyword(s):  
Salivary Gland ◽  
Epithelial Cell ◽  
Cell Mass ◽  
Branching Morphogenesis ◽  
Epithelial Surface ◽  
In Vivo Recombination ◽  
Recombination Experiments

Mouse submaxillary epithelium undergoes branching morphogenesis with increase in the curvature of its surface in vivo. Recombination experiments in vitro of the epithelium and mesenchyme between 13- and 14-day rudiments showed (1) that the 14-day mesenchyme more actively induced the epithelium to branch than the 13-day mesenchyme, (2) that the 14-day mesenchyme could produce clefts on smaller epithelial lobes than the 13-day mesenchyme, (3) that the 14-day mesenchyme produced lobes with similar diameter to lobes of the 14-day intact rudiment, and (4) that the lobular morphology of assembled 14-day lobes became obscure in recombinates with the 13-day mesenchyme while it was well maintained in recombinates with the 14-day mesenchyme. From these results it is concluded that the mesenchyme determines the curvature of epithelial surface, and that clefts are formed on the epithelial surface as a result of increase in the epithelial curvature.

In vivo Imaging of Mammary Epithelial Cell Dynamics in Response to Lineage-Biased Wnt/β-Catenin Activation

2021 ◽  
Author(s):  
Bethan Lloyd-Lewis ◽  
Francesca Gobbo ◽  
Meghan Perkins ◽  
Guillaume Jacquemin ◽  
Marisa M. Faraldo ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Cell Dynamics

scholarly journals The transcription factor GATA6 is essential for branching morphogenesis and epithelial cell differentiation during fetal pulmonary development

Development ◽  
2001 ◽  
Vol 128 (4) ◽  
pp. 503-511
Author(s):  
R. Keijzer ◽  
M. van Tuyl ◽  
C. Meijers ◽  
M. Post ◽  
D. Tibboel ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
Epithelial Cell ◽  
Es Cells ◽  
Embryonic Stem ◽  
Branching Morphogenesis ◽  
Loss Of Function ◽  
Epithelial Cell Differentiation

Recent loss-of-function studies in mice show that the transcription factor GATA6 is important for visceral endoderm differentiation. It is also expressed in early bronchial epithelium and the observation that this tissue does not receive any contribution from Gata6 double mutant embryonic stem (ES) cells in chimeric mice suggests that GATA6 may play a crucial role in lung development. The aim of this study was to determine the role of GATA6 in fetal pulmonary development. We show that Gata6 mRNA is expressed predominantly in the developing pulmonary endoderm and epithelium, but at E15.5 also in the pulmonary mesenchyme. Blocking or depleting GATA6 function results in diminished branching morphogenesis both in vitro and in vivo. TTF1 expression is unaltered in chimeric lungs whereas SPC and CC10 expression are attenuated in abnormally branched areas of chimeric lungs. Chimeras generated in a ROSA26 background show that endodermal cells in these abnormally branched areas are derived from Gata6 mutant ES cells, implicating that the defect is intrinsic to the endoderm. Taken together, these data demonstrate that GATA6 is not essential for endoderm specification, but is required for normal branching morphogenesis and late epithelial cell differentiation.

scholarly journals Btbd7 Regulates Epithelial Cell Dynamics and Branching Morphogenesis

Science ◽  
2010 ◽  
Vol 329 (5991) ◽  
pp. 562-565 ◽  
Author(s):  
T. Onodera ◽  
T. Sakai ◽  
J. C.-f. Hsu ◽  
K. Matsumoto ◽  
J. A. Chiorini ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Branching Morphogenesis ◽  
Cell Dynamics

Electrophysiological Measurements of a Toad Renal Epithelial Cell Line (A6) as an Assay for Predicting Ocular Eye Irritancy

1992 ◽  
Vol 20 (2) ◽  
pp. 218-221
Author(s):  
Henning F. Bjerregaard
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
South African ◽  
Mdck Cells ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Electrophysiological Measurements ◽  
Renal Epithelial Cell Line ◽  
Darby Canine Kidney

An established epithelial cell line (A6) from a South African clawed toad (Xenopus laevis) kidney was used as a model for the corneal epithelium of the eye in order to determine ocular irritancy. When grown on Millipore filter inserts, A6 cells form a monolayer epithelium of high electrical resistance and generate a trans-epithelial potential difference. These two easily-measured electrophysiological endpoints showed a dose-related decrease after exposure for 24 hours to seven selected chemicals of different ocular irritancy potential. It was demonstrated that both trans-epithelial resistance and potential ranked closely with in vivo eye irritancy data and correlated well (r = 0.96) with loss of trans-epithelial impermeability of Madin-Darby canine kidney (MDCK) cells, detected by use of a fluorescein leakage assay.

scholarly journals Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo

2021 ◽  
Vol 14 (8) ◽  
pp. 753
Author(s):  
Anna Matysik-Woźniak ◽  
Waldemar A. Turski ◽  
Monika Turska ◽  
Roman Paduch ◽  
Mirosław Łańcut ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Corneal Epithelium ◽  
Kynurenic Acid ◽  
Pharmaceutical Products ◽  
Corneal Epithelial Cell ◽  
Eye Drops ◽  
Conjunctival Epithelium ◽  
Corneal Erosion

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds. The aim of the present study is to evaluate the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. Normal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. In rabbits, after corneal de-epithelization, eye drops containing 0.002% and 1% KYNA were applied five times a day until full recovery. KYNA decreased metabolism but did not affect the proliferation of the corneal epithelium. It decreased both the metabolism and proliferation of conjunctival epithelium. KYNA enhanced the migration of corneal but not conjunctival epithelial cells. KYNA reduced the secretion of IL-1β and IL-6 from the corneal epithelium, leaving IL-10 secretion unaffected. The release of all studied cytokines from the conjunctival epithelium exposed to KYNA was unchanged. KYNA at higher concentration accelerated the healing of the corneal epithelium. These favorable properties of KYNA suggest that KYNA containing topical pharmaceutical products can be used in the treatment of ocular surface diseases.

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