In vivo Imaging of Mammary Epithelial Cell Dynamics in Response to Lineage-Biased Wnt/β-Catenin Activation

2021 ◽  
Author(s):  
Bethan Lloyd-Lewis ◽  
Francesca Gobbo ◽  
Meghan Perkins ◽  
Guillaume Jacquemin ◽  
Marisa M. Faraldo ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Cell Dynamics

scholarly journals Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173931 ◽  
Author(s):  
Charlène Thiebaut ◽  
Clémence Chamard-Jovenin ◽  
Amand Chesnel ◽  
Chloé Morel ◽  
El-Hadi Djermoune ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Cell Phenotype

Dynamic reciprocity revisited: a continuous, bidirectional flow of information between cells and the extracellular matrix regulates mammary epithelial cell function

1995 ◽  
Vol 73 (7-8) ◽  
pp. 391-397 ◽  
Author(s):  
C. D. Roskelley ◽  
M. J. Bissell
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cell Function ◽  
Mammary Epithelium ◽  
Mammary Epithelial ◽  
Biochemical Signals ◽  
Bidirectional Flow ◽  
Flow Of Information

Interactions between cells and the extracellular matrix (ECM) generate two classes of signals, mechanical and biochemical. In the case of the mammary epithelial cell, both are required to initiate ECM-dependent expression of the abundant milk protein β-casein. Mechanical signals induce a cellular rounding, while functional biochemical signals are associated with an increase in tyrosine phosphorylation. These individual components are part of a complex signalling hierarchy that leads to the emergence of the fully functional lactational phenotype. Interestingly, both the assembly and disassembly of this hierarchy, which occur cyclically in vivo, are constantly modulated by dynamic and reciprocal interactions that take place within a functional unit composed of both the cell and the ECM.Key words: mammary epithelium, differentiation, extracellular matrix, casein.

scholarly journals Progesterone, in Addition to Estrogen, Induces Cyclin D1 Expression in the Murine Mammary Epithelial Cell, in Vivo*

Endocrinology ◽  
1997 ◽  
Vol 138 (9) ◽  
pp. 3933-3939 ◽  
Author(s):  
Thenaa K. Said ◽  
Orla M. Conneely ◽  
Daniel Medina ◽  
Bert W. O’Malley ◽  
John P. Lydon
Keyword(s):  
Epithelial Cell ◽  
Cyclin D1 ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial

scholarly journals Prolactin-mediated regulation of lipid biosynthesis genes in vivo in the lactating mammary epithelial cell

2011 ◽  
Vol 300 (6) ◽  
pp. E1059-E1068 ◽  
Author(s):  
Michael C. Rudolph ◽  
Tanya D. Russell ◽  
Patricia Webb ◽  
Margaret C. Neville ◽  
Steven M. Anderson
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
De Novo ◽  
Morphological Changes ◽  
Pentose Phosphate ◽  
Mammary Epithelial ◽  
Developmental Defects ◽  
Milk Protein Gene

Prolactin (PRL) is known to play an essential role in mammary alveolar proliferation in the pregnant mouse, but its role in lactation has been more difficult to define. Genetic manipulations that alter expression of the PRL receptor and its downstream signaling molecules resulted in developmental defects that may directly or indirectly impact secretory activation and lactation. To examine the in vivo role of PRL specifically in lactation, bromocriptine (BrCr) was administered every 8 h to lactating mice on the second day postpartum, resulting in an ∼95% decrease in serum PRL levels. Although morphological changes in secretory alveoli were slight, by 8 h of BrCr, pup growth was inhibited significantly. Phosphorylated STAT5 fell to undetectable levels within 4 h. Decreased milk protein gene expression, β-casein, and α-lactalbumin, was observed after 8 h of treatment. To assess mammary-specific effects on lipid synthesis genes, we isolated mammary epithelial cells (MECs) depleted of mammary adipocytes. Expression of genes involved in glucose uptake, glycolysis, pentose phosphate shunt, de novo synthesis of fatty acids, and biosynthesis of triacylglycerides was decreased up to 19-fold in MECs by just 8 h of BrCr treatment. Glands from BrCr-treated mice showed a twofold reduction in intracellular cytoplasmic lipid droplets and a reduction in cytosolic β-casein. These data demonstrate that PRL signaling regulates MEC-specific lipogenic gene expression and that PRL signals coordinate the milk synthesis and mammary epithelial cell survival during lactation in the mouse.

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