scholarly journals Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition

Development ◽  
2020 ◽  
Vol 147 (22) ◽  
pp. dev191726
Author(s):  
Dianlei Guo ◽  
Jiali Ru ◽  
Fuxiang Mao ◽  
Hong Ouyang ◽  
Rong Ju ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Cell Polarity ◽  
Planar Cell Polarity ◽  
Drainage System ◽  
Transport Systems ◽  
Cell Axis ◽  
Axis Orientation ◽  
Tear Flow ◽  
Altered Cell

ABSTRACTIn terrestrial animals, the lacrimal drainage apparatus evolved to serve as conduits for tear flow; however, little is known about the ontogenesis of this system. Here, we define the anatomy of the fully formed tear duct in mice, characterize crucial morphogenetic events for the development of tear duct components and identify the site for primordial tear duct (PTD) initiation. We report that the PTD originates from the orbital lacrimal lamina, a junction formed by the epithelia of the maxillary and lateral nasal processes. We demonstrate that Prickle1, a key component of planar cell polarity signaling, is expressed in progenitors of the PTD and throughout tear duct morphogenesis. Disruption of Prickle1 stalls tear duct elongation; in particular, the loss of basement membrane deposition and aberrant cytoplasmic accumulation of laminin are salient. Altered cell adhesion, cytoskeletal transport systems, vesicular transport systems and cell axis orientation in Prickle1 mutants support the role of Prickle1 in planar cell polarity. Taken together, our results highlight a crucial role of Prickle1-mediated polarized basement membrane secretion and deposition in PTD elongation.

scholarly journals Ontogenesis of the tear drainage system requires Prickle 1-controlled polarized basement membrane (BM) deposition

2020 ◽  
Author(s):  
Dianlei Guo ◽  
Jiali Ru ◽  
Fuxiang Mao ◽  
Kaili Wu ◽  
Hong Ouyang ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Protein Trafficking ◽  
Drainage System ◽  
Embryoid Bodies ◽  
Apicobasal Polarity ◽  
Tear Flow ◽  
Cytoplasmic Accumulation ◽  
High Level ◽  
Lacrimal Drainage

AbstractIn terrestrial animals, lacrimal drainage apparatus evolved to serve as conduits for tear flow. Little is known about the ontogenesis of this system. Here, we investigated tear duct origin, developmental course, genetic and cellular determinants in mouse. We report that primordial tear duct (PTD) originates from junction epithelium of the joining maxillary and lateral nasal processes, which reshapes into future tear duct branches. We identified Prickle 1 as a hallmark for tear duct outgrowth, ablation of which stalled duct elongation. In particular, the disruption of basement membrane (BM) with cytoplasmic accumulation of laminin suggests aberrant protein trafficking. Mutant embryoid bodies (EBs) derived from iPSCs recapitulate BM phenotype of the PTD exhibiting defective visceral endoderm (VE), which normally expresses high level of Prickle 1. Furthermore, replenishing mutant VE with Prickle 1 completely rescued BM but not cell polarity. Taken together, our results reveal a distinct role of Prickle 1 in regulating polarized BM secretion and deposition in precedently uncharacterized tear drainage system and VE, which is independent of apicobasal polarity establishment.

Role of the planar cell polarity pathway in regulating ectopic hair cell-like cells induced by Math1 and testosterone treatment

2015 ◽  
Vol 1615 ◽  
pp. 22-30 ◽  
Author(s):  
Xiao-yu Yang ◽  
Kai Jin ◽  
Rui Ma ◽  
Juan-mei Yang ◽  
Wen-wei Luo ◽  
...  
Keyword(s):  
Hair Cell ◽  
Cell Polarity ◽  
Planar Cell Polarity ◽  
Testosterone Treatment ◽  
Planar Cell Polarity Pathway

scholarly journals Planar cell polarity in the larval epidermis of Drosophila and the role of microtubules

Open Biology ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 200290
Author(s):  
Stefano Pietra ◽  
KangBo Ng ◽  
Peter A. Lawrence ◽  
José Casal
Keyword(s):  
Cell Polarity ◽  
Planar Cell Polarity ◽  
Published Data ◽  
Anterior Compartment ◽  
Intercellular Bridges ◽  
Gradient Model

We investigate planar cell polarity (PCP) in the Drosophila larval epidermis. The intricate pattern of denticles depends on only one system of PCP, the Dachsous/Fat system. Dachsous molecules in one cell bind to Fat molecules in a neighbour cell to make intercellular bridges. The disposition and orientation of these Dachsous–Fat bridges allows each cell to compare two neighbours and point its denticles towards the neighbour with the most Dachsous. Measurements of the amount of Dachsous reveal a peak at the back of the anterior compartment of each segment. Localization of Dachs and orientation of ectopic denticles help reveal the polarity of every cell. We discuss whether these findings support our gradient model of Dachsous activity. Several groups have proposed that Dachsous and Fat fix the direction of PCP via oriented microtubules that transport PCP proteins to one side of the cell. We test this proposition in the larval cells and find that most microtubules grow perpendicularly to the axis of PCP. We find no meaningful bias in the polarity of microtubules aligned close to that axis. We also reexamine published data from the pupal abdomen and find no evidence supporting the hypothesis that microtubular orientation draws the arrow of PCP.

Shaping the nervous system: role of the core planar cell polarity genes

2013 ◽  
Vol 14 (8) ◽  
pp. 525-535 ◽  
Author(s):  
Fadel Tissir ◽  
André M. Goffinet
Keyword(s):  
Nervous System ◽  
Cell Polarity ◽  
Planar Cell Polarity ◽  
The Core
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