scholarly journals The atypical histone variant H3.15 promotes callus formation in Arabidopsis thaliana

Development ◽  
2020 ◽  
Vol 147 (11) ◽  
pp. dev184895 ◽  
Author(s):  
An Yan ◽  
Michael Borg ◽  
Frédéric Berger ◽  
Zhong Chen
Keyword(s):  
Arabidopsis Thaliana ◽  
Callus Formation ◽  
Histone Variant

Crosstalk between the cytokinin and MAX2 signaling pathways in growth and callus formation of Arabidopsis thaliana

2019 ◽  
Vol 511 (2) ◽  
pp. 300-306 ◽  
Author(s):  
Weiqiang Li ◽  
Kien Huu Nguyen ◽  
Chien Van Ha ◽  
Yasuko Watanabe ◽  
Lam-Son Phan Tran
Keyword(s):  
Arabidopsis Thaliana ◽  
Signaling Pathways ◽  
Callus Formation

scholarly journals Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Yeong Yeop Jeong ◽  
Hun-Young Lee ◽  
Suk Weon Kim ◽  
Yoo-Sun Noh ◽  
Pil Joon Seo
Keyword(s):  
Tissue Culture ◽  
Arabidopsis Thaliana ◽  
Plant Regeneration ◽  
Tissue Regeneration ◽  
De Novo ◽  
Callus Formation ◽  
Protoplast Regeneration ◽  
Culture Methods ◽  
Cell And Tissue Culture

Abstract Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration. With the growing need for protoplast regeneration in genetic engineering and genome editing, fundamental studies that enhance our understanding of cell cycle re-entry, pluripotency acquisition, and de novo tissue regeneration are essential. To conduct these studies, a reproducible and efficient protoplast regeneration method using model plants is necessary. Results Here, we optimized cell and tissue culture methods for improving protoplast regeneration efficiency in Arabidopsis thaliana. Protoplasts were isolated from whole seedlings of four different Arabidopsis ecotypes including Columbia (Col-0), Wassilewskija (Ws-2), Nossen (No-0), and HR (HR-10). Among these ecotypes, Ws-2 showed the highest potential for protoplast regeneration. A modified thin alginate layer was applied to the protoplast culture at an optimal density of 1 × 106 protoplasts/mL. Following callus formation and de novo shoot regeneration, the regenerated inflorescence stems were used for de novo root organogenesis. The entire protoplast regeneration process was completed within 15 weeks. The in vitro regenerated plants were fertile and produced morphologically normal progenies. Conclusion The cell and tissue culture system optimized in this study for protoplast regeneration is efficient and reproducible. This method of Arabidopsis protoplast regeneration can be used for fundamental studies on pluripotency establishment and de novo tissue regeneration.

scholarly journals Haploid Arabidopsis Thaliana Callus and Plants From Anther Culture

1972 ◽  
Vol 25 (2) ◽  
pp. 259 ◽  
Author(s):  
PM Gresshoff ◽  
CH Doy
Keyword(s):  
Arabidopsis Thaliana ◽  
Hordeum Vulgare ◽  
Callus Formation ◽  
Defined Medium ◽  
Dark Cycle ◽  
Late Prophase ◽  
General Application ◽  
High Auxin ◽  
Haploid Callus ◽  
Prophase Of Meiosis

Haploid callus and plants were cultured from the anthers of diploid A. thaliana. This depends on removing anthers during late prophase of meiosis, selecting a genotype favouring callus formation from dividing sporocytes on a high auxin-low kinetin concentration, fully defined medium, then inducing differentiation by transfer to a low auxin-high kinetin concentration, fully defined medium, with a light-dark cycle. Attempts to produce embryoids directly from' anthers were unsuccessful. The view that our approach may have Ii. more general application is discussed in relation to the work of others and our culture of haploid callus and plantlets from tomato (LycoperBicon eBculentum) and haploid callus from barley (Hordeum vulgare).

scholarly journals High temperature perception in leaves promotes vascular regeneration in distant tissues

2021 ◽  
Author(s):  
Phanu T Serivichyaswat ◽  
Kai Bartusch ◽  
Martina Leso ◽  
Constance Musseau ◽  
Akira Iwase ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Elevated Temperatures ◽  
Callus Formation ◽  
Effect Of Temperature ◽  
Asymmetric Effect ◽  
Ambient Temperatures ◽  
Graft Healing ◽  
Vascular Regeneration ◽  
Xylem Formation ◽  
Temperature Enhancement

Cellular regeneration in response to wounding is fundamental to maintain tissue integrity. Various internal factors including hormones and developmental pathways affect wound healing but little is known about how external factors influence regeneration. To better understand how the environment affects regeneration, we investigated the effects of temperature using the horticulturally relevant process of plant grafting. We found that elevated temperatures accelerated vascular regeneration of Arabidopsis thaliana and tomato (Solanum lycopersicum) grafts. Leaves were critical for this effect since blocking auxin transport or mutating PHYTOCHROME INTERACTING FACTOR4 (PIF4) or YUCCA2/5/8/9 in the cotyledons abolished the temperature enhancement. However, these perturbations had no effect upon graft healing at ambient temperatures and mutations in PIF4 did not affect the temperature enhancement of callus formation or tissue adhesion, suggesting that leaf-derived auxin was specific for enhancing vascular regeneration in response to elevated temperatures. Tissue-specific perturbations of auxin response using a BODENLOS (BDL) mutant revealed an asymmetric effect of temperature upon regeneration: the presence of bdl above the cut prevented temperature enhancement whereas the presence of bdl below the cut prevented graft healing regardless of temperature. Promotion of tissue regeneration by elevated temperatures was not specific for graft healing and we found that elevated temperatures accelerated xylem formation between the parasite Phtheirospermum japonicum and host Arabidopsis thaliana, and this effect required shoot-derived auxin from the parasite. Taken together, our results identify a pathway by which elevated temperatures accelerate vascular development which could be of relevance for improving regeneration and better understanding inter-plant vascular connections.

MYB94 and MYB96 additively inhibit callus formation via directly repressing LBD29 expression in Arabidopsis thaliana

Plant Science ◽  
2020 ◽  
Vol 293 ◽  
pp. 110323 ◽  
Author(s):  
Xuehuan Dai ◽  
Na Liu ◽  
Lijuan Wang ◽  
Juan Li ◽  
Xiaojian Zheng ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Callus Formation

scholarly journals Selection of valine-resistance in callus culture of Arabidopsis thaliana (L.) Heynh. derived from leaf explants

2014 ◽  
Vol 68 (3) ◽  
pp. 211-215
Author(s):  
Małgorzata D. Gaj ◽  
Grzegorz Czaja ◽  
Małgorzata Nawrot
Keyword(s):  
Arabidopsis Thaliana ◽  
In Vitro Selection ◽  
Callus Formation ◽  
Leaf Explants ◽  
Average Frequency ◽  
Leaf Explant ◽  
Regeneration Ability ◽  
Valine Resistance ◽  
Selection Of

The selection of valine-resistant mutants was carried out in leaf explant cultures of three <em>Arabidopsis thaliana</em> (L.) Heynh. ecotypes: C-24, RLD and Columbia. The valine concentration used for in vitro selection, lethal for seed-growing plants, has not affected callus formation and growth. However, strong inhibition of shoot regeneration ability of calli growing under selection pressure was noticed. In total, 1043 explants were cultured on valine medium and 18 shoots were regenerated with an average frequency of 1.7 shoots per 100 calli. Most R<sub>1</sub> shoots were sterile and seeds were collected from 3 plants. The transmission of valine-resistance to the sexual progeny of these plants was scored and the increased level of valine-resistance was found in progeny of one line - 61 C. This line originated from the culture of Columbia leaf explant and displayed tetraploid chromosome number.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Late Flowering ◽  
Transition To Flowering
Sign in / Sign up

Export Citation Format

Share Document