TheDrosophila daughterlessgene autoregulates and is controlled by both positive and negativecisregulation

Development ◽  
2001 ◽  
Vol 128 (23) ◽  
pp. 4705-4714 ◽  
Author(s):  
John E. Smith ◽  
Claire Cronmiller
Keyword(s):  
Negative Regulation ◽  
Fourth Chromosome ◽  
Developmentally Regulated ◽  
Tissue Specific ◽  
Binding Partner ◽  
Helix Loop Helix ◽  
Specific Manner ◽  
Specific Allele ◽  
A Cell ◽  
Bhlh Transcription Factors

As the only class I helix-loop-helix transcription factor in Drosophila, Daughterless (Da) has generally been regarded as a ubiquitously expressed binding partner for other developmentally regulated bHLH transcription factors. From analysis of a novel tissue-specific allele, dalyh, we show that da expression is not constitutive, but is dynamically regulated. This transcriptional regulation includes somatic ovary-specific activation, autoregulation and negative regulation. Unexpectedly, the diverse functions of da may require that expression levels be tightly controlled in a cell and/or tissue-specific manner. Our analysis of dalyh identifies it as the first springer insertion that functions as an insulating element, with its disruptive activity mediated by the product of a fourth chromosome gene, Suppressor of lyh [Su(lyh)].

scholarly journals Syntaxin 1A Gene Is Negatively Regulated in a Cell/Tissue Specific Manner by YY1 Transcription Factor, Which Binds to the −183 to −137 Promoter Region Together with Gene Silencing Factors Including Histone Deacetylase

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Takahiro Nakayama ◽  
Toshiyuki Fukutomi ◽  
Yasuo Terao ◽  
Kimio Akagawa
Keyword(s):  
Transcription Factor ◽  
Gene Silencing ◽  
Protein Complex ◽  
Promoter Region ◽  
Neuronal Cell ◽  
Syntaxin 1A ◽  
Tissue Specific ◽  
Cell Tissue ◽  
Specific Manner ◽  
A Cell

The HPC-1/syntaxin 1A (Stx1a) gene, which is involved in synaptic transmission and neurodevelopmental disorders, is a TATA-less gene with several transcription start sites. It is activated by the binding of Sp1 and acetylated histone H3 to the −204 to +2 core promoter region (CPR) in neuronal cell/tissue. Furthermore, it is depressed by the association of class 1 histone deacetylases (HDACs) to Stx1a–CPR in non-neuronal cell/tissue. To further clarify the factors characterizing Stx1a gene silencing in non-neuronal cell/tissue not expressing Stx1a, we attempted to identify the promoter region forming DNA–protein complex only in non-neuronal cells. Electrophoresis mobility shift assays (EMSA) demonstrated that the −183 to −137 OL2 promoter region forms DNA–protein complex only in non-neuronal fetal rat skin keratinocyte (FRSK) cells which do not express Stx1a. Furthermore, the Yin-Yang 1 (YY1) transcription factor binds to the −183 to −137 promoter region of Stx1a in FRSK cells, as shown by competitive EMSA and supershift assay. Chromatin immunoprecipitation assay revealed that YY1 in vivo associates to Stx1a–CPR in cell/tissue not expressing Stx1a and that trichostatin A treatment in FRSK cells decreases the high-level association of YY1 to Stx1a-CPR in default. Reporter assay indicated that YY1 negatively regulates Stx1a transcription. Finally, mass spectrometry analysis showed that gene silencing factors, including HDAC1, associate onto the −183 to −137 promoter region together with YY1. The current study is the first to report that Stx1a transcription is negatively regulated in a cell/tissue-specific manner by YY1 transcription factor, which binds to the −183 to −137 promoter region together with gene silencing factors, including HDAC.

scholarly journals XASH genes promote neurogenesis in Xenopus embryos

Development ◽  
1994 ◽  
Vol 120 (12) ◽  
pp. 3649-3655 ◽  
Author(s):  
B. Ferreiro ◽  
C. Kintner ◽  
K. Zimmerman ◽  
D. Anderson ◽  
W.A. Harris
Keyword(s):  
Neural Development ◽  
Neural Induction ◽  
Neural Tissue ◽  
Neural Plate ◽  
Binding Partner ◽  
Helix Loop Helix ◽  
Neural Genes ◽  
Dorsal Ectoderm ◽  
Bhlh Transcription Factors ◽  
Beta Tubulin Gene

Neural development in Drosophila is promoted by a family of basic helix-loop-helix (bHLH) transcription factors encoded within the Achaete Scute-Complex (AS-C). XASH-3, a Xenopus homolog of the Drosophila AS-C genes, is expressed during neural induction within a portion of the dorsal ectoderm that gives rise to the neural plate and tube. Here, we show that XASH-3, when expressed with the promiscuous binding partner XE12, specifically activates the expression of neural genes in naive ectoderm, suggesting that XASH-3 promotes neural development. Moreover, XASH-3/XE12 RNA injections into embryos lead to hypertrophy of the neural tube. Interestingly, XASH-3 misexpression does not lead to the formation of ectopic neural tissue in ventral regions, suggesting that the domain of XASH proneural function is restricted in the embryo. In contrast to the neural inducer noggin, which permanently activates the NCAM gene, the activation of neural genes by XASH-3/XE12 is not stable in naive ectoderm, yet XASH-3/XE12 powerfully and stably activates NCAM, Neurofilament and type III beta-tubulin gene expression in noggin-treated ectoderm. These results show that the XASH-3 promotes neural development, and suggest that its activity depends on additional factors which are induced in ectoderm by factors such as noggin.

Follistatin modulates activin activity in a cell- and tissue-specific manner.

Endocrinology ◽  
1993 ◽  
Vol 132 (6) ◽  
pp. 2732-2734 ◽  
Author(s):  
J P Mather ◽  
P E Roberts ◽  
L A Krummen
Keyword(s):  
Tissue Specific ◽  
Specific Manner ◽  
A Cell

Hypertension Increases Connexin43 in a Tissue-Specific Manner

Circulation ◽  
1997 ◽  
Vol 95 (4) ◽  
pp. 1007-1014 ◽  
Author(s):  
Jacques-Antoine Haefliger ◽  
Einar Castillo ◽  
Ge´rard Waeber ◽  
Gabriela E. Bergonzelli ◽  
Jean-Franc¸ois Aubert ◽  
...  
Keyword(s):  
Tissue Specific ◽  
Specific Manner

scholarly journals Tentacle Morphological Variation Coincides with Differential Expression of Toxins in Sea Anemones

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 452
Author(s):  
Lauren M. Ashwood ◽  
Michela L. Mitchell ◽  
Bruno Madio ◽  
David A. Hurwood ◽  
Glenn F. King ◽  
...  
Keyword(s):  
Differential Expression ◽  
Morphological Variation ◽  
Expression Profiles ◽  
The Body ◽  
Sea Anemones ◽  
Tissue Specific ◽  
Venom Composition ◽  
Specific Manner ◽  
Branched Structures ◽  
Morphological Variants

Phylum Cnidaria is an ancient venomous group defined by the presence of cnidae, specialised organelles that serve as venom delivery systems. The distribution of cnidae across the body plan is linked to regionalisation of venom production, with tissue-specific venom composition observed in multiple actiniarian species. In this study, we assess whether morphological variants of tentacles are associated with distinct toxin expression profiles and investigate the functional significance of specialised tentacular structures. Using five sea anemone species, we analysed differential expression of toxin-like transcripts and found that expression levels differ significantly across tentacular structures when substantial morphological variation is present. Therefore, the differential expression of toxin genes is associated with morphological variation of tentacular structures in a tissue-specific manner. Furthermore, the unique toxin profile of spherical tentacular structures in families Aliciidae and Thalassianthidae indicate that vesicles and nematospheres may function to protect branched structures that host a large number of photosynthetic symbionts. Thus, hosting zooxanthellae may account for the tentacle-specific toxin expression profiles observed in the current study. Overall, specialised tentacular structures serve unique ecological roles and, in order to fulfil their functions, they possess distinct venom cocktails.

scholarly journals Retinoid nutritional status differently affects the expression of Japanese quail retinoic acid receptor-beta isoform transcripts in a tissue-specific manner

2001 ◽  
Vol 169 (2) ◽  
pp. 281-290 ◽  
Author(s):  
ZW Fu ◽  
T Kubo ◽  
K Sugahara ◽  
T Noguchi ◽  
H Kato
Keyword(s):  
Retinoic Acid ◽  
Nutritional Status ◽  
Japanese Quail ◽  
Retinoic Acid Receptor ◽  
Mrna Levels ◽  
Tissue Specific ◽  
Specific Manner ◽  
Lower Magnitude ◽  
Lung And Kidney ◽  
Receptor Beta

We investigated the effects of vitamin A (VA) nutritional status on the levels of expression of retinoic acid (RA) receptor-beta (RARbeta) gene in the various tissues of Japanese quail. VA deficiency caused a significant decrease in the mRNA levels of brain, liver, heart, lung and kidney RARbeta2/beta4, whereas no change was observed in the level of testis RARbeta2 transcript. In contrast, reduction in the RARbeta1 transcript caused by VA depletion was observed only in the lung, remaining unchanged in the other tissues. The administration of RA to the VA-deficient quail rapidly induced the expression of RARbeta2/beta4 mRNAs in all the tissues examined, but RA increased the expression of RARbeta1 transcript in the liver, heart, lung and kidney at a lower magnitude. RA could not change the expression of the brain RARbeta1 transcript, while it induced the expression of the testis RARbeta1 mRNA in a temporal way. These results clearly indicate that VA nutritional status differently regulates the expression of RARbeta1 and RARbeta2/beta4 transcripts in a tissue-specific manner.

W1763 Colonic Expression of Claudin-2 Increases in Colitis and Colitis Associated Cancer (Cac) and is Regulated by EGF Receptor Activation in a Tissue Specific Manner

2010 ◽  
Vol 138 (5) ◽  
pp. S-735
Author(s):  
Rupesh Chaturvedi ◽  
Rizwan Ahmad ◽  
Mohammad Asim ◽  
Kay Washington ◽  
Keith T. Wilson ◽  
...  
Keyword(s):  
Egf Receptor ◽  
Receptor Activation ◽  
Tissue Specific ◽  
Specific Manner

ERRATUM

Development ◽  
1988 ◽  
Vol 104 (4) ◽  
pp. 525-s-525
Keyword(s):  
National Cancer Institute ◽  
Health Sciences ◽  
Experimental Carcinogenesis ◽  
Developmentally Regulated ◽  
Tissue Specific

The authors made an error in the institution address for the article: The expression of rat homeobox-containing genes is developmentally regulated and tissue specific in Development103, 601–610 (1988) The correct institution address should read: MIRIAM FALZON1 and SU YUN CHUNG Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, MD 20892 and Department of Biochemistry, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA Note: Present address: Laboratory of Biochemistry. National Cancer Institute, Bethesda. MD 20892, USA

scholarly journals Rel Induces Interferon Regulatory Factor 4 (IRF-4) Expression in Lymphocytes

2000 ◽  
Vol 191 (8) ◽  
pp. 1281-1292 ◽  
Author(s):  
Raelene J. Grumont ◽  
Steve Gerondakis
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Nuclear Factor Κb ◽  
Wild Type ◽  
Cell Type ◽  
Regulatory Factor ◽  
Specific Manner ◽  
A Cell ◽  
Cell Type Specific ◽  
Interferon Regulatory Factor 4

In lymphocytes, the Rel transcription factor is essential in establishing a pattern of gene expression that promotes cell proliferation, survival, and differentiation. Here we show that mitogen-induced expression of interferon (IFN) regulatory factor 4 (IRF-4), a lymphoid-specific member of the IFN family of transcription factors, is Rel dependent. Consistent with IRF-4 functioning as a repressor of IFN-induced gene expression, the absence of IRF-4 expression in c-rel−/− B cells coincided with a greater sensitivity of these cells to the antiproliferative activity of IFNs. In turn, enforced expression of an IRF-4 transgene restored IFN modulated c-rel−/− B cell proliferation to that of wild-type cells. This cross-regulation between two different signaling pathways represents a novel mechanism that Rel/nuclear factor κB can repress the transcription of IFN-regulated genes in a cell type–specific manner.

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