Lis1, the Drosophila homolog of a human lissencephaly disease gene, is required for germline cell division and oocyte differentiation

Development ◽  
1999 ◽  
Vol 126 (20) ◽  
pp. 4477-4488 ◽  
Author(s):  
Z. Liu ◽  
T. Xie ◽  
R. Steward
Keyword(s):  
Cell Division ◽  
Neuronal Migration ◽  
Nuclear Migration ◽  
Germline Cell ◽  
Causal Gene ◽  
Membrane Cytoskeleton ◽  
Drosophila Homolog ◽  
Congenital Brain Malformation ◽  
Development Analysis ◽  
Oocyte Differentiation

Lissencephaly is a severe congenital brain malformation resulting from incomplete neuronal migration. One causal gene, LIS1, is homologous to nudF, a gene required for nuclear migration in A. nidulans. We have characterized the Drosophila homolog of LIS1 (Lis1) and show that Lis1 is essential for fly development. Analysis of ovarian Lis1 mutant clones demonstrates that Lis1 is required in the germline for synchronized germline cell division, fusome integrity and oocyte differentiation. Abnormal packaging of the cysts was observed in Lis1 mutant clones. Our results indicate that LIS1 is important for cell division and differentiation and the function of the membrane cytoskeleton. They support the notion that LIS1 functions with the dynein complex to regulate nuclear migration or cell migration.

Asymmetric cell division and differentiation; fern spore germination as a model. II. Ultrastructural studies

1985 ◽  
Vol 86 ◽  
pp. 227-230
Author(s):  
Alix R. Bassel
Keyword(s):  
Cell Division ◽  
Metal Binding ◽  
Asymmetric Cell Division ◽  
Red Light ◽  
Nuclear Migration ◽  
Silver Stain ◽  
Metal Binding Sites ◽  
Ultrastructural Studies ◽  
Fern Spore Germination

SynopsisThe germination of Onoclea spores is a model system with many advantages for the study of asymmetric cell division and cellular differentiation. Our results suggest that both microtubules and a lipophilic site are important in the nuclear migration to one end of the spore prior to asymmetric cell division. A metalbinding region containing pore-like structures in the proximal face of the spore coat may be a source of the inherent polarity of the spore. The pattern of endogenous metal binding during germination has been characterised using a sulphide-silver stain. Metal-binding sites are described in a differentiating system in which polarity is imposed externally using polarised red light. The possibility of a role of ion gradients in determining the direction of nuclear migration is discussed.

scholarly journals NudF, a nuclear migration gene in Aspergillus nidulans, is similar to the human LIS-1 gene required for neuronal migration.

1995 ◽  
Vol 6 (3) ◽  
pp. 297-310 ◽  
Author(s):  
X Xiang ◽  
A H Osmani ◽  
S A Osmani ◽  
M Xin ◽  
N R Morris
Keyword(s):  
Brain Development ◽  
Aspergillus Nidulans ◽  
Gene Product ◽  
Filamentous Fungus ◽  
Neuronal Migration ◽  
Protein Level ◽  
Vegetative Growth ◽  
Nuclear Migration ◽  
Strong Similarity ◽  
Sequence Identity

During a study of the genetics of nuclear migration in the filamentous fungus Aspergillus nidulans, we cloned a gene, nudF, which is required for nuclear migration during vegetative growth as well as development. The NUDF protein level is controlled by another protein NUDC, and extra copies of the nudF gene can suppress the nudC3 mutation. nudF encodes a protein with 42% sequence identity to the human LIS-1 (Miller-Dieker lissencephaly-1) gene, which is required for proper neuronal migration during brain development. This strong similarity suggests that the LIS-1 gene product may have a function similar to that of NUDF and supports previous findings to suggest that nuclear migration may play a role in neuronal migration.

scholarly journals LIS1 at the microtubule plus end and its role in dynein-mediated nuclear migration

2003 ◽  
Vol 160 (3) ◽  
pp. 289-290 ◽  
Author(s):  
Xin Xiang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Migration ◽  
Cytoplasmic Dynein ◽  
Causal Gene ◽  
New Findings ◽  
Dynactin Complex

The cytoplasmic dynein complex and its accessory dynactin complex are involved in many cellular activities including nuclear migration in fungi (for review see Karki and Holzbaur, 1999). LIS1, the product of a causal gene for human lissencephaly (smooth brain), has also been implicated in dynein function based on studies in fungi and more recent studies in higher eukaryotic systems (for review see Gupta et al., 2002). Exactly how LIS1 may regulate the behavior of cytoplasmic dynein in various organisms is a fascinating question. In this issue, Lee et al. (2003) describe important new findings in Saccharomyces cerevisiae regarding the role of LIS1 (Pac1) in dynein-mediated nuclear migration.

scholarly journals Rapid accumulation of mutations in growing mycelia of a hypervariable fungus Schizophyllum commune

2019 ◽  
Author(s):  
Aleksandra V. Bezmenova ◽  
Elena A. Zvyagina ◽  
Anna V. Fedotova ◽  
Artem S. Kasianov ◽  
Tatiana V. Neretina ◽  
...  
Keyword(s):  
Cell Division ◽  
Mutation Rate ◽  
De Novo ◽  
Schizophyllum Commune ◽  
Natural Populations ◽  
Germline Cell ◽  
Cell Divisions ◽  
Mutation Burden ◽  
Armillaria Gallica ◽  
Very High

AbstractThe number of mutations that occur per nucleotide per generation varies between species by several orders of magnitude. In multicellular eukaryotes, the per generation mutation rate depends both on the per cell division mutation rate and on the number of germline cell divisions per generation. In a range of species, from fungi to humans, the number of germline cell divisions is lower than that of somatic cells, reducing the mutation burden on the offspring. The basidiomycete Schizophyllum commune has the highest level of genetic polymorphism known among eukaryotes. In a previous study, it was also found to have a high per generation mutation rate, probably contributing to its high polymorphism. However, this rate has been measured only in a breeding experiment on Petri dishes, and it is unclear how this result translates to natural populations. Here, we used an experimental design that measures the rate of accumulation of de novo mutations in a linearly growing mycelium. We show that S. commune accumulates mutations at a uniform rate of 1.4·10−7 substitutions per nucleotide per meter of growth, which is 3 orders of magnitude higher than the corresponding rates in the oak Quercus robur and the fungus Armillaria gallica. This figure is consistent with the estimate obtained before, and suggests the lack of a dedicated germline in this system. If so, even a low per cell division mutation rate can translate into a very high per generation mutation rate when the number of cell divisions between consecutive meioses is large.

scholarly journals Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity

2015 ◽  
Vol 32 (2) ◽  
pp. 203-219 ◽  
Author(s):  
Paulina J. Strzyz ◽  
Hyun O. Lee ◽  
Jaydeep Sidhaye ◽  
Isabell P. Weber ◽  
Louis C. Leung ◽  
...  
Keyword(s):  
Cell Division ◽  
Apical Cell ◽  
Nuclear Migration ◽  
Tissue Integrity ◽  
Interkinetic Nuclear Migration
Sign in / Sign up

Export Citation Format

Share Document