scholarly journals MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 609-620 ◽  
Author(s):  
L. Lo ◽  
M.C. Tiveron ◽  
D.J. Anderson
Keyword(s):  
Transcription Factor ◽  
Neuronal Differentiation ◽  
Large Fraction ◽  
Constitutive Expression ◽  
Neuronal Morphology ◽  
Bone Morphogenetic Protein 2 ◽  
General Idea ◽  
Homeodomain Transcription Factor ◽  
Neuronal Markers ◽  
Neuronal Identity

We have investigated the genetic circuitry underlying the determination of neuronal identity, using mammalian peripheral autonomic neurons as a model system. Previously, we showed that treatment of neural crest stem cells (NCSCs) with bone morphogenetic protein-2 (BMP-2) leads to an induction of MASH1 expression and consequent autonomic neuronal differentiation. We now show that BMP2 also induces expression of the paired homeodomain transcription factor Phox2a, and the GDNF/NTN signalling receptor tyrosine kinase c-RET. Constitutive expression of MASH1 in NCSCs from a retroviral vector, in the absence of exogenous BMP2, induces expression of both Phox2a and c-RET in a large fraction of infected colonies, and also promotes morphological neuronal differentiation and expression of pan-neuronal markers. In vivo, expression of Phox2a in autonomic ganglia is strongly reduced in Mash1 −/− embryos. These loss- and gain-of-function data suggest that MASH1 positively regulates expression of Phox2a, either directly or indirectly. Constitutive expression of Phox2a, by contrast to MASH1, fails to induce expression of neuronal markers or a neuronal morphology, but does induce expression of c-RET. These data suggest that MASH1 couples expression of pan-neuronal and subtype-specific components of autonomic neuronal identity, and support the general idea that identity is established by combining subprograms involving cascades of transcription factors, which specify distinct components of neuronal phenotype.

scholarly journals Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Xiaoqing Song ◽  
Jin Dai ◽  
Huaguang Li ◽  
Yuemeng Li ◽  
Weixiao Hao ◽  
...  
Keyword(s):  
Neuronal Differentiation ◽  
Neuron Specific Enolase ◽  
Neuronal Morphology ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Aging Effects ◽  
Neuroprotective Effects ◽  
Self Renewal ◽  
Neuronal Markers ◽  
Wide Range

AbstractIn order to improve the therapeutic effects of mesenchymal stem cell (MSC)-based therapies for a number of intractable neurological disorders, a more favorable strategy to regulate the outcome of bone marrow MSCs (bMSCs) was examined in the present study. In view of the wide range of neurotrophic and neuroprotective effects, Tetramethylpyrazine (TMP), a biologically active alkaloid isolated from the herbal medicine Ligusticum wallichii, was used. It was revealed that treatment with 30–50 mg/l TMP for 4 days significantly increased cell viability, alleviated senescence by suppressing NF-κB signaling, and promoted bMSC proliferation by regulating the cell cycle. In addition, 40–50 mg/l TMP treatment may facilitate the neuronal differentiation of bMSCs, verified in the present study by presentation of neuronal morphology and expression of neuronal markers: microtubule-associated protein 2 (MAP-2) and neuron-specific enolase (NSE). The quantitative real-time polymerase chain reaction (qRT-PCR) revealed that TMP treatment may promote the expression of neurogenin 1 (Ngn1), neuronal differentiation 1 (NeuroD) and mammalian achaete–scute homolog 1 (Mash1). In conclusion, 4 days of 40–50 mg/l TMP treatment may significantly delay bMSC senescence by suppressing NF-κB signaling, and enhancing the self-renewal ability of bMSCs, and their potential for neuronal differentiation.

scholarly journals Homeodomain Transcription Factor Phox2a, via Cyclic AMP-Mediated Activation, Induces p27Kip1 Transcription, Coordinating Neural Progenitor Cell Cycle Exit and Differentiation

2006 ◽  
Vol 26 (23) ◽  
pp. 8826-8839 ◽  
Author(s):  
Maryline Paris ◽  
Wen-Horng Wang ◽  
Min-Hwa Shin ◽  
David S. Franklin ◽  
Ourania M. Andrisani
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Cyclic Amp ◽  
Neuronal Differentiation ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Camp Signaling ◽  
Cell Cycle Exit ◽  
Homeodomain Transcription Factor

ABSTRACT Mechanisms coordinating neural progenitor cell cycle exit and differentiation are incompletely understood. The cyclin-dependent kinase inhibitor p27Kip1 is transcriptionally induced, switching specific neural progenitors from proliferation to differentiation. However, neuronal differentiation-specific transcription factors mediating p27Kip1 transcription have not been identified. We demonstrate the homeodomain transcription factor Phox2a, required for central nervous system (CNS)- and neural crest (NC)-derived noradrenergic neuron differentiation, coordinates cell cycle exit and differentiation by inducing p27Kip1 transcription. Phox2a transcription and activation in the CNS-derived CAD cell line and primary NC cells is mediated by combined cyclic AMP (cAMP) and bone morphogenetic protein 2 (BMP2) signaling. In the CAD cellular model, cAMP and BMP2 signaling initially induces proliferation of the undifferentiated precursors, followed by p27Kip1 transcription, G1 arrest, and neuronal differentiation. Small interfering RNA silencing of either Phox2a or p27Kip1 suppresses p27Kip1 transcription and neuronal differentiation, suggesting a causal link between p27Kip1 expression and differentiation. Conversely, ectopic Phox2a expression via the Tet-off expression system promotes accelerated CAD cell neuronal differentiation and p27Kip1 transcription only in the presence of cAMP signaling. Importantly, endogenous or ectopically expressed Phox2a activated by cAMP signaling binds homeodomain cis-acting elements of the p27Kip1 promoter in vivo and mediates p27Kip1-luciferase expression in CAD and NC cells. We conclude that developmental cues of cAMP signaling causally link Phox2a activation with p27Kip1 transcription, thereby coordinating neural progenitor cell cycle exit and differentiation.

The role of Phox2b in synchronizing pan-neuronal and type-specific aspects of neurogenesis

Development ◽  
2002 ◽  
Vol 129 (22) ◽  
pp. 5241-5253
Author(s):  
Véronique Dubreuil ◽  
Marie-Rose Hirsch ◽  
Caroline Jouve ◽  
Jean-François Brunet ◽  
Christo Goridis
Keyword(s):  
Nervous System ◽  
Transcription Factor ◽  
Neuronal Differentiation ◽  
Genetic Interactions ◽  
Homeodomain Transcription Factor ◽  
Dual Action ◽  
Separate Pathway ◽  
Neuronal Subtype ◽  
Necessary And Sufficient

Within the developing vertebrate nervous system, specific subclasses of neurons are produced in vastly different numbers at defined times and locations. This implies the concomitant activation of a program that controls pan-neuronal differentiation and of a program that specifies neuronal subtype identity, but how these programs are coordinated in time and space is not well understood. Our previous loss- and gain-of-function studies have defined Phox2b as a homeodomain transcription factor that coordinately regulates generic and type-specific neuronal properties. It is necessary and sufficient to impose differentiation towards a branchio- and viscero-motoneuronal phenotype and at the same time promotes generic neuronal differentiation. We have examined the underlying genetic interactions. We show thatPhox2b has a dual action on pan-neuronal differentiation. It upregulates the expression of proneural genes (Ngn2) when expressed alone and upregulates the expression of Mash1 when expressed in combination with Nkx2.2. By a separate pathway, Phox2brepresses expression of the inhibitors of neurogenesis Hes5 andId2. The role of Phox2b in the specification of neuronal subtype identity appears to depend in part on its capacity to act as a patterning gene in the progenitor domain. Phox2b misexpression represses the Pax6 and Olig2 genes, which should inhibit a branchiomotor fate, and induces Nkx6.1 and Nkx6.2, which are expressed in branchiomotor progenitors. We further show that Phox2b behaves like a transcriptional activator in the promotion of both, generic neuronal differentiation and expression of the motoneuronal marker Islet1. These results provide insights into the mechanisms by which a homeodomain transcription factor through interaction with other factors controls both generic and type-specific features of neuronal differentiation.

The transcription factor activating protein 2 beta (TFAP2B) mediates neuronal differentiation in neuroblastoma cells

2013 ◽  
Vol 225 (03) ◽  
Author(s):  
F Sherkheli ◽  
S Ackermann ◽  
F Roels ◽  
H Kocak ◽  
R Volland ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neuronal Differentiation ◽  
Neuroblastoma Cells

scholarly journals A crucial role for the homeodomain transcription factor Hhex in lymphopoiesis

Blood ◽  
2015 ◽  
Vol 125 (5) ◽  
pp. 803-814 ◽  
Author(s):  
Jacob T. Jackson ◽  
Chayanica Nasa ◽  
Wei Shi ◽  
Nicholas D. Huntington ◽  
Clifford W. Bogue ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Crucial Role ◽  
Homeodomain Transcription Factor ◽  
Key Points

Key Points Hhex regulates development of diverse lymphoid lineages. Hhex regulates cycling of lymphoid precursors.

scholarly journals The PDX1 Homeodomain Transcription Factor Negatively Regulates the Pancreatic Ductal Cell-specific Keratin 19 Promoter

2006 ◽  
Vol 281 (50) ◽  
pp. 38385-38395 ◽  
Author(s):  
Therese B. Deramaudt ◽  
Mira M. Sachdeva ◽  
Melanie P. Wescott ◽  
Yuting Chen ◽  
Doris A. Stoffers ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Homeodomain Transcription Factor ◽  
Keratin 19 ◽  
Ductal Cell ◽  
Pancreatic Ductal Cell
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