scholarly journals Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Xiaoqing Song ◽  
Jin Dai ◽  
Huaguang Li ◽  
Yuemeng Li ◽  
Weixiao Hao ◽  
...  
Keyword(s):  
Neuronal Differentiation ◽  
Neuron Specific Enolase ◽  
Neuronal Morphology ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Aging Effects ◽  
Neuroprotective Effects ◽  
Self Renewal ◽  
Neuronal Markers ◽  
Wide Range

AbstractIn order to improve the therapeutic effects of mesenchymal stem cell (MSC)-based therapies for a number of intractable neurological disorders, a more favorable strategy to regulate the outcome of bone marrow MSCs (bMSCs) was examined in the present study. In view of the wide range of neurotrophic and neuroprotective effects, Tetramethylpyrazine (TMP), a biologically active alkaloid isolated from the herbal medicine Ligusticum wallichii, was used. It was revealed that treatment with 30–50 mg/l TMP for 4 days significantly increased cell viability, alleviated senescence by suppressing NF-κB signaling, and promoted bMSC proliferation by regulating the cell cycle. In addition, 40–50 mg/l TMP treatment may facilitate the neuronal differentiation of bMSCs, verified in the present study by presentation of neuronal morphology and expression of neuronal markers: microtubule-associated protein 2 (MAP-2) and neuron-specific enolase (NSE). The quantitative real-time polymerase chain reaction (qRT-PCR) revealed that TMP treatment may promote the expression of neurogenin 1 (Ngn1), neuronal differentiation 1 (NeuroD) and mammalian achaete–scute homolog 1 (Mash1). In conclusion, 4 days of 40–50 mg/l TMP treatment may significantly delay bMSC senescence by suppressing NF-κB signaling, and enhancing the self-renewal ability of bMSCs, and their potential for neuronal differentiation.

scholarly journals The application of resveratrol to mesenchymal stromal cell-based regenerative medicine

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Chenxia Hu ◽  
Lanjuan Li
Keyword(s):  
Regenerative Medicine ◽  
Tissue Injury ◽  
Lineage Commitment ◽  
Root Extract ◽  
Therapeutic Effects ◽  
Aging Effects ◽  
Chronic Injury ◽  
Self Renewal

Abstract Currently, the transplantation of mesenchymal stromal cells (MSCs) has emerged as an effective strategy to protect against tissue and organ injury. MSC transplantation also serves as a promising therapy for regenerative medicine, while poor engraftment and limited survival rates are major obstacles for its clinical application. Although multiple studies have focused on investigating chemicals to improve MSC stemness and differentiation in vitro and in vivo, there is still a shortage of effective and safe agents for MSC-based regenerative medicine. Resveratrol (RSV), a nonflavonoid polyphenol phytoalexin with a stilbene structure, was first identified in the root extract of white hellebore and is also found in the roots of Polygonum cuspidatum, and it is widely used in traditional Chinese medicine. RSV is a natural agent that possesses great therapeutic potential for protecting against acute or chronic injury in multiple tissues as a result of its antioxidative, anti-inflammatory, and anti-cancer properties. According to its demonstrated properties, RSV may improve the therapeutic effects of MSCs via enhancing their survival, self-renewal, lineage commitment, and anti-aging effects. In this review, we concluded that RSV significantly improved the preventive and therapeutic effects of MSCs against multiple diseases. We also described the underlying mechanisms of the effects of RSV on the survival, self-renewal, and lineage commitment of MSCs in vitro and in vivo. Upon further clarification of the potential mechanisms of the effects of RSV on MSC-based therapy, MSCs may be able to be more widely used in regenerative medicine to promote recovery from tissue injury.

scholarly journals MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 609-620 ◽  
Author(s):  
L. Lo ◽  
M.C. Tiveron ◽  
D.J. Anderson
Keyword(s):  
Transcription Factor ◽  
Neuronal Differentiation ◽  
Large Fraction ◽  
Constitutive Expression ◽  
Neuronal Morphology ◽  
Bone Morphogenetic Protein 2 ◽  
General Idea ◽  
Homeodomain Transcription Factor ◽  
Neuronal Markers ◽  
Neuronal Identity

We have investigated the genetic circuitry underlying the determination of neuronal identity, using mammalian peripheral autonomic neurons as a model system. Previously, we showed that treatment of neural crest stem cells (NCSCs) with bone morphogenetic protein-2 (BMP-2) leads to an induction of MASH1 expression and consequent autonomic neuronal differentiation. We now show that BMP2 also induces expression of the paired homeodomain transcription factor Phox2a, and the GDNF/NTN signalling receptor tyrosine kinase c-RET. Constitutive expression of MASH1 in NCSCs from a retroviral vector, in the absence of exogenous BMP2, induces expression of both Phox2a and c-RET in a large fraction of infected colonies, and also promotes morphological neuronal differentiation and expression of pan-neuronal markers. In vivo, expression of Phox2a in autonomic ganglia is strongly reduced in Mash1 −/− embryos. These loss- and gain-of-function data suggest that MASH1 positively regulates expression of Phox2a, either directly or indirectly. Constitutive expression of Phox2a, by contrast to MASH1, fails to induce expression of neuronal markers or a neuronal morphology, but does induce expression of c-RET. These data suggest that MASH1 couples expression of pan-neuronal and subtype-specific components of autonomic neuronal identity, and support the general idea that identity is established by combining subprograms involving cascades of transcription factors, which specify distinct components of neuronal phenotype.

scholarly journals Neuroprotective Effects of Cornus officinalis on Stress-Induced Hippocampal Deficits in Rats and H2O2-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Weishun Tian ◽  
Jing Zhao ◽  
Jeong-Ho Lee ◽  
Md Rashedunnabi Akanda ◽  
Jeong-Hwi Cho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Forced Swim Test ◽  
Neuroblastoma Cells ◽  
Mitogen Activated Protein Kinase ◽  
Ros Generation ◽  
Neuroprotective Effects ◽  
Neuronal Markers ◽  
Cornus Officinalis ◽  
Immobility Time ◽  
Wide Range

Oxidative stress plays a vital role in neurodegenerative diseases. Cornus officinalis (CC) has a wide range of pharmacological activities (e.g., antioxidant, neuroprotective, and anti-inflammatory). The present study was undertaken to elucidate the neuroprotective mechanism of CC and fermented CC (FCC) on stress and H2O2-induced oxidative stress damage in rats and SH-SY5Y cells. A dose of 100 mg/kg CC or FCC was orally administered to rats 1 h prior to immobilization 2 h per day for 14 days. CC, especially FCC administration decreased immobility time in forced swim test (FST), effectively alleviated the oxidative stress, and remarkably decreased corticosterone, β-endorphin and increased serotonin levels, respectively. In cells, CC and FCC significantly inhibited reactive oxygen species (ROS) generation, lactate dehydrogenase (LDH) release and significantly increased the genes expression of antioxidant and neuronal markers, such as superoxide dismutase (SOD), catalase (CAT), and brain-derived neurotrophic factor (BDNF). Moreover, the pro-apoptotic factor Bax and anti-apoptotic factor Bcl-2 (Bax/Bcl-2) ratio was regulated by CC and FCC pretreatment. Both in rats and cells, CC and FCC downregulated mitogen-activated protein kinase (MAPK) phosphorylation. Taken together, these results demonstrated that CC and particularly FCC ameliorated oxidative stress and may be used on the neuroprotection.

scholarly journals Cannabidiol: from an inactive cannabinoid to a drug with wide spectrum of action

2008 ◽  
Vol 30 (3) ◽  
pp. 271-280 ◽  
Author(s):  
Antonio Waldo Zuardi
Keyword(s):  
Chemical Structure ◽  
Web Of Science ◽  
Inflammatory Diseases ◽  
Wide Spectrum ◽  
Therapeutic Effects ◽  
Neuroprotective Effects ◽  
Wide Range ◽  
Sedative Effects ◽  
Therapeutic Properties ◽  
Number Of Publications

OBJECTIVE: The aim of this review is to describe the historical development of research on cannabidiol. METHOD: This review was carried out on reports drawn from Medline, Web of Science and SciELO. DISCUSSION: After the elucidation of the chemical structure of cannabidiol in 1963, the initial studies showed that cannabidiol was unable to mimic the effects of Cannabis. In the 1970's the number of publications on cannabidiol reached a first peak, having the research focused mainly on the interaction with delta9-THC and its antiepileptic and sedative effects. The following two decades showed lower degree of interest, and the potential therapeutic properties of cannabidiol investigated were mainly the anxiolytic, antipsychotic and on motor diseases effects. The last five years have shown a remarkable increase in publications on cannabidiol mainly stimulated by the discovery of its anti-inflammatory, anti-oxidative and neuroprotective effects. These studies have suggested a wide range of possible therapeutic effects of cannabidiol on several conditions, including Parkinson's disease, Alzheimer's disease, cerebral ischemia, diabetes, rheumatoid arthritis, other inflammatory diseases, nausea and cancer. CONCLUSION: In the last 45 years it has been possible to demonstrate that CBD has a wide range of pharmacological effects, many of which being of great therapeutic interest, but still waiting to be confirmed by clinical trials.

Tangeretin: a mechanistic review of its pharmacological and therapeutic effects

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
Milad Ashrafizadeh ◽  
Zahra Ahmadi ◽  
Reza Mohammadinejad ◽  
Elham Ghasemipour Afshar
Keyword(s):  
Diabetes Mellitus ◽  
Side Effects ◽  
Fruits And Vegetables ◽  
Biological Activities ◽  
Therapeutic Effects ◽  
Neuroprotective Effects ◽  
Synthetic Drugs ◽  
Treatment And Prevention ◽  
Naturally Occurring ◽  
Wide Range

AbstractTo date, a large number of synthetic drugs have been developed for the treatment and prevention of different disorders, such as neurodegenerative diseases, diabetes mellitus, and cancer. However, these drugs suffer from a variety of drawbacks including side effects and low efficacy. In response to this problem, researchers have focused on the plant-derived natural products due to their valuable biological activities and low side effects. Flavonoids consist of a wide range of naturally occurring compounds exclusively found in fruits and vegetables and demonstrate a number of pharmacological and therapeutic effects. Tangeretin (TGN) is a key member of flavonoids that is extensively found in citrus peels. It has different favorable biological activities such as antioxidant, anti-inflammatory, antitumor, hepatoprotective, and neuroprotective effects. In the present review, we discuss the various pharmacological and therapeutic effects of TGN and then, demonstrate how this naturally occurring compound affects signaling pathways to exert its impacts.

DEVELOPMENT OF THE COMPOSITION AND TECHNOLOGY OF SEDATIVE FILMS

2021 ◽  
pp. 162-168
Author(s):  
Zamakhaeva E.A. ◽  
Oleshko O.A. ◽  
Yaborova O.V. ◽  
Smirnova M.M. ◽  
Kuritsyn A.V.
Keyword(s):  
Medicinal Plant ◽  
Raw Materials ◽  
Biological Fluid ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Plant Raw Materials ◽  
Number Of Patients ◽  
Wide Range ◽  
Active Substances

Herbal sedatives have been popular for several centuries, and the accumulated knowledge that has been passed down from generation to generation laid the foundation for the development of traditional medicine, and over time for such a science as herbal medicine. Herbal sedatives have a wide range of therapeutic effects, are well tolerated and have no serious adverse reactions, and are not addictive. According to statistical data on the incidence of the population by main classes and individual diseases for 2010-2018, the number of patients with diseases of the nervous system is growing annually, along with this, the need for sedative drugs is increasing. In this regard, the search and development of effective and safe medicines based on medicinal plant raw materials is an urgent area [1, 2]. Pharmaceutical infusions and decoctions, according to the order of the Ministry of Health of the Russian Federation No. 751n dated October 26, 2015, have a shelf life of 2 days, which significantly limits the possibility of their use. The production of films based on aqueous extracts from medicinal plant raw materials will make it possible to obtain more stable drugs, in addition, films have a number of advantages over other dosage forms. Films containing biologically active substances of plant origin have received the unofficial name phytofilms, which are a type of transdermal therapeutic systems. Phytofilms are hydrophilic systems that swell when in contact with water (or biological fluid), absorbing it in a certain amount, which leads to the dissolution of biologically active substances. They differ from synthetic polymeric therapeutic systems in that phytofilms are made in the form of matrix transdermal therapeutic systems on carriers of natural origin (gelatin, collagen, sodium alginate, agar-agar, etc.), which makes them safer [3, 4].

BIOTECHNOLOGICAL PROCESS OF CREATING FUNCTIONAL DAIRY PRODUCT ENRICHED WITH THE EXTRACT OF SCUTELLARIA GALERICULATA L.

2020 ◽  
Author(s):  
O.V. Belashova ◽  
◽  
A.V. Zaushintsena ◽  
N.V. Fotina
Keyword(s):  
Dairy Product ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Scutellaria Baicalensis Georgi ◽  
Physico Chemical ◽  
Functional Product ◽  
Wide Range ◽  
Active Substances

One of the ways to eliminate population diseases and increase the body is resistance to adverse environmental factors is the systematic use of functional food products enriched with a complex of biologically active substances with a wide range of therapeutic effects. The creation of a new, unique functional dairy product was preceded by long-term comparative biochemical studies of Scutellaria baicalensis Georgi and Scutellaria galericulata L. In the grass and roots, biologically active substances of a phenolic nature, such as baicalin, vogonin, scutellarin, etc., were found useful for medicinal purposes. The use of extracts from the of Scutellaria galericulata L. in the development of a functional product based on sour-milk fractions made it possible to obtain curd mass enriched with the biologically active substance of this plant. The functional product meets the organoleptic, physico-chemical, tasting requirements for new functional products and is recommended for introduction into the diet of different population groups by age and functional activity.

scholarly journals STUDY OF THE BIOTECHNOLOGICAL POTENTIAL OF SELECTED LACTIC ACID BACTERIA CULTURES

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
N. Kigel ◽  
I. Melnik ◽  
O. Naumenko
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Biologically Active ◽  
Protoplast Regeneration ◽  
Therapeutic Effects ◽  
Biotechnological Potential ◽  
Milk Clotting ◽  
Wide Range

Fermenting microflora has been selected by biotechnological activity markers, with various methodological approaches used, namely: directional selection, selection of bacteriophage-insensitive mutants, protoplast regeneration. The experimental data show a significant biotechnological potential of the selected lactic acid bacteria. They are characterized by high milk-clotting activity and yield, the ability to form aromatic compounds and/or viscous components, excellent organoleptic qualities of clots fermented by them, antago-nistic activity against pathogenic and opportunistic pathogenic microorganisms, and phage-resistance to species-specific virulent phages. The collection of industrial microorganisms of Institute of Food Resources of National Academy of Agrarian Sciences (IFR NAAS) has been supplemented with new bioactive strains, in particular, the species Streptococcus thermophilus, Lactobacillus acidophilus, Lactoba-cillus rhamnosus and Lactococcus lactis. Four bacterial compositions have been created. Three of them (Iprovit-LB-R; Iprovit-LB-A, and Iprovit-ST) are supposed to be used as functional enrichers for dry foodstuffs or as biologically active supplements. The bacterial prepara-tion Iprovit-Bifidolux is a universal composition. It can be introduced both as a fermenting culture for milk fermentation and as an enricher for dry and liquid foodstuffs. Biotechnologies for the production of dry bacterial preparations on the basis of selected strains have been field-proven at the State Research Enterprise of Starter Cultures that belongs to the IFR NAAS. The data on a wide range of clinical and therapeutic effects of the bacterial preparation Iprovit-Bifidolux allow recommending it for manufacturing functional foods.

Aspergillus sydowii Strain SCAU066 and Aspergillus versicolor Isolate BAB-6580: Potential Source of Xylanolytic, Cellulolytic and Amylolytic Enzymes

2020 ◽  
Vol 14 (2) ◽  
pp. 15
Author(s):  
Zaidah Zainal ariffin
Keyword(s):  
Extracellular Enzymes ◽  
Biologically Active ◽  
Aspergillus Versicolor ◽  
Aspergillus Sydowii ◽  
Aspergillus Tamarii ◽  
Alternative Source ◽  
Amylolytic Enzymes ◽  
Potato Dextrose Broth ◽  
Wide Range ◽  
Pharmaceutical Industries

Fungi is known to produce a wide range of biologically active metabolites and enzymes. Enzymes produced by fungi are utilized in food and pharmaceutical industries because of their rich enzymatic profile. Filamentous fungi are particularly interesting due to their high production of extracellular enzymes which has a large industrial potential. The aim of this study is to isolate potential soil fungi species that are able to produce functional enzymes for industries. Five Aspergillus species were successfully isolated from antibiotic overexposed soil (GPS coordinate of N3.093219 E101.40269) by standard microbiological method. The isolated fungi were identified via morphological observations and molecular tools; polymerase chain reactions, ITS 1 (5’- TCC GTA GGT GAA CCT GCG G3’) forward primer and ITS 4 (5’-TCC TCC GCT TAT TGA TAT GC-3’) reverse primer. The isolated fungi were identified as Aspergillus sydowii strain SCAU066, Aspergillus tamarii isolate TN-7, Aspergillus candidus strain KUFA 0062, Aspergillus versicolor isolate BAB-6580, and Aspergillus protuberus strain KAS 6024. Supernatant obtained via submerged fermentation of the isolated fungi in potato dextrose broth (PDB) and extracted via centrifugation was loaded onto specific media to screen for the production of xylanolytic, cellulolytic and amylolytic enzymes. The present findings indicate that Aspergillus sydowii strain SCAU066 and Aspergillus versicolor isolate BAB-6580 have great potential as an alternative source of xylanolytic, cellulolytic and amylolytic enzymes.

The content of decenoic acids in drone brood preparations and combined preparations based on it

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 389-393
Author(s):  
D.V. Mitrofanov ◽  
N.V. Budnikova
Keyword(s):  
Royal Jelly ◽  
Storage Conditions ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Strict Adherence ◽  
Drone Brood ◽  
Wide Range ◽  
Active Substances ◽  
Melanin Complex

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

Sign in / Sign up

Export Citation Format

Share Document