scholarly journals Segregating expression domains of two goosecoid genes during the transition from gastrulation to neurulation in chick embryos

Development ◽  
1997 ◽  
Vol 124 (8) ◽  
pp. 1443-1452 ◽  
Author(s):  
L. Lemaire ◽  
T. Roeser ◽  
J.C. Izpisua-Belmonte ◽  
M. Kessel
Keyword(s):  
Posterior Margin ◽  
Anterior Part ◽  
Primitive Streak ◽  
Neural Plate ◽  
Chick Embryos ◽  
Positive Part ◽  
Full Extension ◽  
Isolation And Characterization ◽  
Chick Embryogenesis

We report the isolation and characterization of a chicken gene, GSX, containing a homeobox similar to that of the goosecoid gene. The structure of the GSX gene and the deduced GSX protein are highly related to the previously described goosecoid gene. The two homeodomains are 74% identical. In the first few hours of chick embryogenesis, the expression pattern of GSX is similar to GSC, in the posterior margin of the embryo and the young primitive streak. Later during gastrulation, expression of the two genes segregate. GSC is expressed in the anterior part of the primitive streak, then in the node, and finally in the pre-chordal plate. GSX is expressed in the primitive streak excluding the node, and then demarcating the early neural plate around the anterior streak and overlying the pre-chordal plate. We demonstrate that the GSX-positive part of the primitive streak induces gastrulation, while the GSC-expressing part induces neurulation. After full extension of the streak, the fate of cells now characterized by GSX is to undergo neurulation, while those expressing GSC undergo gastrulation. We discuss the effect of a duplicated basic goosecoid identity for the generation of a chordate nervous system in ontogeny and phylogeny.

scholarly journals Experiments on Embryonic Induction

1934 ◽  
Vol 11 (3) ◽  
pp. 212-217
Author(s):  
C. H. WADDINGTON
Keyword(s):  
Primitive Streak ◽  
Neural Plate ◽  
Chick Embryos ◽  
Embryonic Induction ◽  
Gut Endoderm

It is shown that the ectoderm of the area opaca of chick embryos of the primitive streak stage can react to primitive streak grafts by the formation of an induced neural plate. The conclusion is drawn that the competence to form neural plate is not conferred on ectoderm by the gut endoderm, which determines the formation of the primitive streak.

scholarly journals Induction by the Primitive Streak and its Derivatives in the Chick

1933 ◽  
Vol 10 (1) ◽  
pp. 38-46 ◽  
Author(s):  
C. H. WADDINGTON
Keyword(s):  
Anterior Part ◽  
Primitive Streak ◽  
Neural Plate ◽  
Embryonic Axis ◽  
Host Embryo

1. Specimens are described which demonstrate the induction of neural plateby (a) the mesodermal part of the primitive streak, (b) the head process and sinus rhomboidalis, and (c) neural plate. 2. The neural plate which was induced by the mesodermal part of the primitive streak was in reversed orientation as regards the host embryo. Thus the orientation of the embryo must be already fixed in the mesodermal part of the streak, and must in this case have overcome any influence which the host may be able to exert. 3. The same embryo was more complete than indicated by the presumptive fate of the tissue which induced it, whence it is concluded that the chick organiser,like the amphibian, shows a tendency to complete itself, and to this extent behaves like part of a harmonious equipotential system. 4. Grafts of the anterior part of the embryonic axis (head process and neuralplate) into the anterior part of the host blastoderm, have induced structures which in nearly all cases give indications of being heads. Inductions by posterior parts of the axis (sinus rhomboidalis) have never given such indications. 5. Grafts of the notochord have not, as yet, given satisfactory inductions.

scholarly journals Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yuhua Gao ◽  
Chunyu Bai ◽  
Hui Xiong ◽  
Qian Li ◽  
Zhiqiang Shan ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Skin Tissue ◽  
Chick Embryos ◽  
Multilineage Differentiation ◽  
Rt Pcr ◽  
Ideal Candidate ◽  
Isolation And Characterization ◽  
Cellular Transplantation ◽  
Transplantation Therapy

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression ofβ-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.

scholarly journals Isolation and characterization of collagen A and B chains from chick embryos

FEBS Letters ◽  
1979 ◽  
Vol 99 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Helga Von Der Mark ◽  
Klaus Von Der Mark
Keyword(s):  
Chick Embryos ◽  
Isolation And Characterization

Isolation and Characterization of Collagen - Synthesizing Polysomes from Chick Embryos

1975 ◽  
Vol 5 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Wang L. ◽  
Andrade H. J. ◽  
Silva S. M. F. ◽  
Sim[otilde]es C. L. ◽  
Ďabronzo F. H. ◽  
...  
Keyword(s):  
Chick Embryos ◽  
Isolation And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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