scholarly journals Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yuhua Gao ◽  
Chunyu Bai ◽  
Hui Xiong ◽  
Qian Li ◽  
Zhiqiang Shan ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Skin Tissue ◽  
Chick Embryos ◽  
Multilineage Differentiation ◽  
Rt Pcr ◽  
Ideal Candidate ◽  
Isolation And Characterization ◽  
Cellular Transplantation ◽  
Transplantation Therapy

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression ofβ-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.

Isolation and Characterization of Mesenchymal Stem Cells from Chicken Liver

2020 ◽  
Vol 10 (1) ◽  
pp. 8-16
Author(s):  
Xibin Liu ◽  
Shuang Zhang ◽  
Weijun Guan ◽  
Dong Zheng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Markers ◽  
Multilineage Differentiation ◽  
Rt Pcr ◽  
Multipotent Stem Cells ◽  
Isolation And Characterization ◽  
Potential Applications ◽  
Polymerase Chain

Hepatic mesenchymal stem cells (HMSCs) are multipotent stem cells that is a vital part of the regeneration of hepatocytes after injury. In this study, HMSCs were isolated in embryonic livers from of 12-day-old chick embryo using collagenase, and the primary HMSCs were sub-cultured to passage. The protein markers of HMSCs, namely CD71, CD29 and CD44, were tested with immunofluorescence and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The proliferation of HMSCs in different passages was detected using growth curve, which shown a typically sigmoidal. And then, the pluripotent of HMSCs was analyzed, the results showed that HMSCs could directly induce to differentiate into neural-like cells, adipocytes, and osteoblasts. Our data illustrated that the chick HMSCs have same characteristics to those obtained from other species. The capacity of these cells for multilineage differentiation shows promise for many potential applications.

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties

2007 ◽  
Vol 313 (5) ◽  
pp. 1008-1023 ◽  
Author(s):  
Mitsutaka Shiota ◽  
Toshio Heike ◽  
Munetada Haruyama ◽  
Shiro Baba ◽  
Atsunori Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Distal Lung Progenitor Cells

2012 ◽  
pp. 109-122 ◽  
Author(s):  
Barbara Driscoll ◽  
Alex Kikuchi ◽  
Allison N. Lau ◽  
Jooeun Lee ◽  
Raghava Reddy ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Isolation And Characterization ◽  
Distal Lung ◽  
Lung Progenitor

Isolation and characterization of murine neural stem/progenitor cells based on Prominin-1 expression

2007 ◽  
Vol 205 (2) ◽  
pp. 547-562 ◽  
Author(s):  
Stefania Corti ◽  
Monica Nizzardo ◽  
Martina Nardini ◽  
Chiara Donadoni ◽  
Federica Locatelli ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Isolation And Characterization ◽  
Neural Stem Progenitor Cells

300 ESTABLISHMENT AND CHARACTERIZATION OF RAT MESENCHYMAL STEM CELLS

2011 ◽  
Vol 23 (1) ◽  
pp. 247
Author(s):  
T. H. Kim ◽  
B. G. Jeon ◽  
S. L. Lee ◽  
G. J. Rho
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcriptional Factors ◽  
Skin Tissue ◽  
Rt Pcr ◽  
Alternative Source ◽  
Differentiated Cells ◽  
Fetal Bovine

Mesenchymal stem cells (MSC) are regarded as an attractive source for tissue engineering and regeneration, and bone marrow extract has been commonly used as a source of pluripotent MSC. However, skin tissue has recently been identified as a convenient alternative source of MSC. The present study was focused on the effect of characterised MSC derived from rat on expression of early transcriptional factors, alkaline phosphate (AP) activity, and in vitro differentiation into selected cell lineages. The MSC were isolated from 8-week-old s.d. rat’s ear skin and cultured in advanced DMEM supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere of 5% CO2 in air. To evaluate AP activity, cells were fixed with 3.7% formaldehyde solution and stained with Western Blue® (Promega, Madison, WI, USA). Expressions of early transcriptional factors (Oct-4, Sox2, and Nanog) were evaluated by RT-PCR. Differentiation into distinct mesenchymal lineages such as adipogenic, osteogenic, and neuron was done by following previously described protocols and assessed by lineage-specific stains. The specific genes in the osteocytes (osteocalcin, osteonectin, osteopontin, and Runx2), adipocytes (pparγ2, adiponectin, and aP2) or neuron (nestin, neurogenin 1, β-tublin, and nerve growth factor) were characterised by RT-PCR. The MSC were positive for AP activity and expressed Oct-4, Sox2, and Nanog. Following induction, MSC were successfully differentiated into adipocytes, osteocytes, and neurons. As adipocytes markers, aP2, pparγ2, and adiponectin were strongly detected in the adipocyte induced cells. Osteonectin, osteocalcin, Runx2, and osteopontin were expressed in the adipocyte induced cells. Futhermore, neuron-specific markers were clearly expressed in the neuronal differentiated cells. In conclusion, MSC have the capability of differentiation into multilineages including adipocytes, osteocytes, and neurons under the specific induction conditions. Skin tissue in rat can serve as an easily accessible and expandable alternative source for MSC harvesting and preclinical applications using an animal model. This work was supported by Grant No. 2007031034040 from Bio-organ and 200908FHT010204005 from Biogreen21, Republic of Korea.

scholarly journals Isolation and characterization of neural progenitor cells from post-mortem human cortex

2003 ◽  
Vol 74 (6) ◽  
pp. 838-851 ◽  
Author(s):  
Philip H. Schwartz ◽  
Peter J. Bryant ◽  
Tannin J. Fuja ◽  
Hailing Su ◽  
Diane K. O'Dowd ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Post Mortem ◽  
Human Cortex ◽  
Isolation And Characterization
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