scholarly journals Detection of nerve growth factor mRNA in the developing chicken embryo

Development ◽  
1988 ◽  
Vol 102 (1) ◽  
pp. 101-106 ◽  
Author(s):  
T. Ebendal ◽  
H. Persson
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Chicken Embryo ◽  
Sensory Innervation ◽  
Cholinergic Innervation ◽  
Mrna Levels ◽  
Peripheral Tissues ◽  
Nerve Growth ◽  
Early Peak ◽  
The Brain

Nerve growth factor (beta NGF) is a protein supporting sympathetic and sensory innervation in the peripheral tissues as well as cholinergic innervation in the brain. A DNA probe derived from a genomic clone coding for chicken NGF was used to study NGF mRNA levels during development. NGF mRNA was detected in the chicken embryo as early as day 3.5 of incubation. The level of NGF mRNA in total embryo increased four-fold until day 8, remained high until day 12, and subsequently decreased. No corresponding peak in NGF mRNA expression was found in heart and brain measured separately. Instead these organs showed increased NGF mRNA levels after hatching. The highest levels of NGF mRNA in the day-8 embryo were found in skin and eye (in particular cornea, but also iris, sclera-choroid and neural retina) suggesting a correlation between sensory innervation and this early peak of NGF expression.

scholarly journals Studies on the regulation of beta-nerve growth factor gene expression in the rat iris: the level of mRNA-encoding nerve growth factor is increased in irises placed in explant cultures in vitro, but not in irises deprived of sensory or sympathetic innervation in vivo.

1986 ◽  
Vol 102 (5) ◽  
pp. 1940-1948 ◽  
Author(s):  
D L Shelton ◽  
L F Reichardt
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sympathetic Innervation ◽  
Sensory Innervation ◽  
Surgical Removal ◽  
Mrna Levels ◽  
Nerve Growth ◽  
Rat Iris

Beta-nerve growth factor (NGF) is a protein necessary for the survival and maintenance of sympathetic and sensory neurons that appears to be produced by the target tissues of these neurons in vivo. Both denervation and the culture of explants of one model target, the rat iris, leads to an increase in the NGF content, suggesting that innervating neurons may regulate a step in synthesis or turnover of NGF. To determine whether there is a change in synthesis controlled at the mRNA level, the rat iris has been assayed for its content of NGF mRNA after surgical and chemical denervation and after explant into culture. Using a sensitive blot hybridization assay, a large, rapid increase in the content of NGF mRNA was observed upon explant of the rat iris. The increase was readily detectable within 1 h, reached a maximum increase of 10- to 20-fold by 6 to 12 h, and was still evident after 3 d in culture. The distribution of NGF mRNA in different areas of the iris does not change during this time. This rapid increase in NGF mRNA is also seen in the fully innervated iris in vivo after trauma to the anterior chamber. In contrast, denervation to varying degrees in situ had no effect on NGF mRNA levels. Neither removal of sympathetic innervation by surgical or chemical methods nor combined surgical removal of sympathetic and sensory innervation detectably altered NGF mRNA content. Thus, denervation of the rat iris in situ does not cause the observed accumulation of NGF by increasing the level of NGF mRNA, and the increase in NGF content must be due to other factors.

scholarly journals Modulatory role of platelet-derived growth factor on cytokine-induced nerve growth factor synthesis in rat glomerular mesangial cells

1995 ◽  
Vol 312 (3) ◽  
pp. 707-711 ◽  
Author(s):  
K M A Plüss ◽  
J Pfeilschifter ◽  
H Mühl ◽  
A Huwiler ◽  
C Boeckh ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mesangial Cells ◽  
Interleukin 1 ◽  
Platelet Derived Growth Factor ◽  
Tnf Alpha ◽  
Mrna Levels ◽  
Glomerular Mesangial Cells ◽  
Peripheral Tissues ◽  
Nerve Growth

Recent evidence indicates that cytokines are potent inducers of nerve growth factor (NGF) expression in peripheral tissues and in brain. Cultured rat glomerular mesangial cells respond to interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) by increased NGF synthesis. We found that co-stimulation of rat glomerular mesangial cells with platelet-derived growth factor (PGDF-BB) and IL-1 beta/TNF-alpha significantly augments the IL-1 beta/TNF-alpha-induced NGF mRNA levels and NGF synthesis. In contrast, preincubation with PDGF-BB drastically reduces NGF gene expression and NGF protein synthesis in response to IL-1 beta/TNF-alpha stimulation. Thus our results indicate that PDGF-BB is a potent modulator of cytokine-induced NGF expression; its precise action is critically depending on the time at which the PDGF receptor is activated.

A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis

2015 ◽  
Vol 25 (6) ◽  
pp. 931-936 ◽  
Author(s):  
Takanori Omae ◽  
Junichi Nakamura ◽  
Seiji Ohtori ◽  
Sumihisa Orita ◽  
Kazuyo Yamauchi ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Model ◽  
Inflammatory Arthritis ◽  
Hip Pain ◽  
Sensory Innervation ◽  
Nerve Growth
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