scholarly journals Notch-mediated patterning and cell fate allocation of pancreatic progenitor cells

Development ◽  
2012 ◽  
Vol 139 (10) ◽  
pp. 1744-1753 ◽  
Author(s):  
S. Afelik ◽  
X. Qu ◽  
E. Hasrouni ◽  
M. A. Bukys ◽  
T. Deering ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells

scholarly journals Notch mediated patterning and cell fate allocation of pancreatic progenitor cells

2011 ◽  
Vol 356 (1) ◽  
pp. 168
Author(s):  
Solomon Afelik ◽  
Xioling Qu ◽  
Edy Hasrouni ◽  
Michael Bukys ◽  
Stephan Niewoudt ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells

scholarly journals Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells Promote the Proliferation and Differentiation of Pancreatic Progenitor Cells and the Engraftment Function of Islet-Like Cell Clusters

2019 ◽  
Vol 20 (17) ◽  
pp. 4083
Author(s):  
Xing Yu Li ◽  
Shang Ying Wu ◽  
Po Sing Leung
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Progenitor Cells ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells ◽  
Proliferation And Differentiation ◽  
Differentiation And Proliferation

Pancreatic progenitor cells (PPCs) are the primary source for all pancreatic cells, including beta-cells, and thus the proliferation and differentiation of PPCs into islet-like cell clusters (ICCs) opens an avenue to providing transplantable islets for diabetic patients. Meanwhile, mesenchymal stem cells (MSCs) can enhance the development and function of different cell types of interest, but their role on PPCs remains unknown. We aimed to explore the mechanism-of-action whereby MSCs induce the in vitro and in vivo PPC/ICC development by means of our established co-culture system of human PPCs with human fetal bone marrow-derived MSCs. We examined the effect of MSC-conditioned medium on PPC proliferation and survival. Meanwhile, we studied the effect of MSC co-culture enhanced PPC/ICC function in vitro and in vivo co-/transplantation. Furthermore, we identified IGF1 as a critical factor responsible for the MSC effects on PPC differentiation and proliferation via IGF1-PI3K/Akt and IGF1-MEK/ERK1/2, respectively. In conclusion, our data indicate that MSCs stimulated the differentiation and proliferation of human PPCs via IGF1 signaling, and more importantly, promoted the in vivo engraftment function of ICCs. Taken together, our protocol may provide a mechanism-driven basis for the proliferation and differentiation of PPCs into clinically transplantable islets.

scholarly journals Genetic Modification of Primate Amniotic Fluid-Derived Stem Cells Produces Pancreatic Progenitor Cells in vitro

2013 ◽  
Vol 197 (4) ◽  
pp. 269-282 ◽  
Author(s):  
Yu Zhou ◽  
David L. Mack ◽  
J. Koudy Williams ◽  
Sayed-Hadi Mirmalek-Sani ◽  
Emily Moorefield ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Genetic Modification ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells
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