scholarly journals Developmental expression patterns of toolkit genes in male accessory gland of Drosophila parallels those of mammalian prostate

Biology Open ◽  
2021 ◽  
Author(s):  
Jaya Kumari ◽  
Pradip Sinha
Keyword(s):  
Fibroblast Growth Factor Receptor ◽  
Expression Patterns ◽  
Growth Factor Receptor ◽  
Accessory Gland ◽  
Developmental Expression ◽  
Fibroblast Growth ◽  
Male Accessory Gland ◽  
Hox Gene ◽  
Sexual Organ ◽  
The Individual

Conservation of genetic toolkits in disparate phyla may help reveal commonalities in organ designs transcending their extreme anatomical disparities. A male accessory sexual organ in mammals, the prostate, for instance, is anatomically disparate from its analogous, phylogenetically distant counterpart—the male accessory gland (MAG)—in insects like Drosophila. It has not been ascertained if the anatomically disparate Drosophila MAG shares developmental parallels with those of the mammalian prostate. Here we show that the development of Drosophila mesoderm-derived MAG entails recruitment of similar genetic toolkits of tubular organs like that seen in endoderm-derived mammalian prostate. For instance, like mammalian prostate, Drosophila MAG morphogenesis is marked by recruitment of fibroblast growth factor receptor, FGFR—a signalling pathway often seen recruited for tubulogenesis—starting early during its adepithelial genesis. A specialisation of the individual domains of the developing MAG tube, on the other hand, is marked by the expression of a posterior Hox gene transcription factor, Abd-B, while Hh-Dpp signalling marks its growth. Drosophila MAG, therefore, reveals the developmental design of a unitary bud-derived tube that appears to have been co-opted for the development of male accessory sexual organs across distant phylogeny and embryonic lineages.

scholarly journals DrosophilaMale Accessory Gland Displays Developmental Ground Plan of a Primitive Prostate

2021 ◽  
Author(s):  
Jaya Kumari ◽  
Pradip Sinha
Keyword(s):  
Fibroblast Growth Factor Receptor ◽  
Expression Patterns ◽  
Growth Factor Receptor ◽  
Accessory Gland ◽  
Male Accessory Gland ◽  
Developmental Genetic ◽  
Evolutionary Diversification ◽  
Ground Plan ◽  
Deep Homology ◽  
The Individual

AbstractConservation of developmental genetic toolkits of functionally comparable organs from disparate phyla reveals their deep homology, which may help overcome the challenges of their confounding categorization as either homologous or analogous organs. A male accessory sexual organ in mammals, prostate, for instance, is anatomically disparate from its phylogenetically distant counterpart—the male accessory gland (MAG)—in insects likeDrosophila. By examining a select set of toolkit gene expression patterns, here we show thatDrosophilaMAG displays deep homology with the mammalian prostate. Like mammalian prostate, MAG morphogenesis is marked by recruitment of fibroblast growth factor receptor, FGFR, a tubulogenesis toolkit signaling pathway, starting early during its adepithelial genesis. Specialization of the individual domains of the developing MAG tube on the other hand is marked by expression of a posterior Hox gene transcription factor, Abd-B, while Hh-Dpp signaling marks its growth.DrosophilaMAG thus reveals developmental design of unitary bud-derived tube—a ground plan that appears to have been reiteratively co-opted during evolutionary diversification of male accessory sexual organs across distant phylogeny.

Expression Patterns of Two Fibroblast Growth Factor Receptor Genes during Early Chick Eye Development

1994 ◽  
Vol 58 (6) ◽  
pp. 649-658 ◽  
Author(s):  
Hideyo Ohuchi ◽  
Eiki Koyama ◽  
Fumio Myokai ◽  
Tsutomu Nohno ◽  
Fumio Shiraga ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Eye Development ◽  
Expression Patterns ◽  
Growth Factor Receptor ◽  
Fibroblast Growth

212 EXPRESSION OF FIBROBLAST GROWTH FACTOR 18 (FGF18) AND COGNATE RECEPTORS (FGFR3C AND FGFR4) DURING LUTEAL DEVELOPMENT AND INDUCED LUTEOLYSIS IN CATTLE

2010 ◽  
Vol 22 (1) ◽  
pp. 264
Author(s):  
D. M. Guerra ◽  
A. C. S. Castilho ◽  
M.F. Machado ◽  
B. Berisha ◽  
D. Schams ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Blood Vessels ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Expression Patterns ◽  
Growth Factor Receptor ◽  
Immunohistochemical Analysis ◽  
Fibroblast Growth ◽  
Santa Cruz

Fibroblast growth factor receptor 2 (FGFR2) has been shown to induce luteinization in granulosa cells, luteal angiogenesis, and luteal growth. Alternative splicing of 4 genes give rise to 7 subtypes of fibroblast growth factor receptors (FGFR) with varying affinity for different fibroblast growth factors (FGF). Fibroblast growth factor receptor 2 and FGF18 efficiently activate FGFR3C and FGFR4 and may act in cooperation in tissues expressing these receptors. We aimed to determine mRNA expression patterns for FGF18, FGFR3C, and FGFR4 during bovine luteal development and following induced luteolysis. In addition, we assessed FGF18 localization in the bovine CL. Bovine CL were obtained from abattoir ovaries and classed into 4 stages of development: stage 1 =corpus hemorragicum; stage 2 = developing CL; stage 3 = mature or early functional luteolysis CL; and stage 4 = structural luteolysis. To assess FGF18 and FGFR mRNA expression during induced luteolysis, adult cows (Bos taurus Holstein-Friesians) were injected with the PGF2 analogue cloprostenol (500 mg i.m. Intervet, Unterschleissheim, Germany) during the mid-luteal phase of the cycle (Days 8-12). Corpus luteum were collected by transvaginal ovariectomy at 0, 0.5, 2, 4, 12, 24, 48, and 64 hr (n = 5/time point) after PGF2 injection. Tissue samples were submitted to total RNA extraction. Expression of FGF18, FGFR3C, and 4 mRNA during the bovine CL lifespan and induced luteolysis were measured by real-time RT-PCR with oligo-dT in the RT and bovine-specific primers in the PCR. Expression of cyclophilin was used as internal control. The effect of developmental stage and time post-PGF2 on gene expression was tested by ANOVA, followed by Tukey- Kramer HSD test. Immunohistochemical analysis was performed with a commercial human antibody (anti-FGF18; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Fibroblast growth factor 18, FGFR3C, and FGFR4 mRNA was detected in all 4 developmental stages; FGF18 mRNA abundance was higher in stage 3 (2.89 ± 0.05; mean ± SEM) compared with stages 1 (0.3 ± 0.27), 2 (0.56 ± 1.27), and 4 (0.99 ± 0.32). Fibroblast growth factor 18 and FGFR4 mRNA expression did not significantly change during induced luteolysis. Fibroblast growth factor receptor 3C mRNA abundance peaked 4 h after PGF2 injection and significantly decreased at 24 h post-treatment in comparison with peak levels. Immunohistochemical analysis revealed the presence of FGF18 in small and large luteal cells and in blood vessels. In conclusion, the mRNA expression patterns of FGF18 and its receptors suggest their participation in the control of luteal differentiation, particularly during functional luteolysis. The localization of FGF18 protein to blood vessels suggests it may play a role in the control of angiogenesis in the bovine CL. Supported by CAPES/FAPESP.

Distinct developmental expression of a new avian fibroblast growth factor receptor

Development ◽  
1994 ◽  
Vol 120 (3) ◽  
pp. 683-694 ◽  
Author(s):  
C. Marcelle ◽  
A. Eichmann ◽  
O. Halevy ◽  
C. Breant ◽  
N.M. Le Douarin
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Satellite Cells ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Primitive Streak ◽  
Developmental Expression ◽  
Fibroblast Growth ◽  
Fibroblast Growth Factor Receptors

We have cloned a new member of the fibroblast growth factor receptor family from avian embryonic RNA. The FREK (for fibroblast growth factor receptor-like embryonic kinase) primary transcript can be alternatively spliced in a tissue- and stage-specific manner to give rise to molecules containing either two or three Ig-like domains. During elongating primitive streak stages, FREK is expressed in the rostral and lateral epiblast and in the Hensen's node. From 2.5 days of development (E 2.5) on, it is expressed in various ectoderm- and mesoderm-derived structures. Most striking is FREK expression in the skeletal muscle lineage. It is highly expressed in the early myotome and, at later stages, in all skeletal muscles of the embryo. From E9 to hatching, FREK expression in the muscles decreases dramatically but is maintained in satellite cells of adult muscles. FREK transcript is elevated upon addition of basic fibroblast growth factor to serum-starved satellite cells. From this study, we conclude: (1) that the structure and pattern of expression of FREK set it apart from other cloned fibroblast growth factor receptors (FGFR) and suggest that FREK is a new member of that family; (2) that FREK may play multiple roles in early avian development, including a specialized role in the early differentiation of skeletal muscle.

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