Molecular cloning and developmental expression of rat fibroblast growth factor receptor 3

2001 ◽  
Vol 115 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Peter Claus ◽  
Claudia Grothe
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Molecular Cloning ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Developmental Expression ◽  
Fibroblast Growth

Distinct developmental expression of a new avian fibroblast growth factor receptor

Development ◽  
1994 ◽  
Vol 120 (3) ◽  
pp. 683-694 ◽  
Author(s):  
C. Marcelle ◽  
A. Eichmann ◽  
O. Halevy ◽  
C. Breant ◽  
N.M. Le Douarin
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Satellite Cells ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Primitive Streak ◽  
Developmental Expression ◽  
Fibroblast Growth ◽  
Fibroblast Growth Factor Receptors

We have cloned a new member of the fibroblast growth factor receptor family from avian embryonic RNA. The FREK (for fibroblast growth factor receptor-like embryonic kinase) primary transcript can be alternatively spliced in a tissue- and stage-specific manner to give rise to molecules containing either two or three Ig-like domains. During elongating primitive streak stages, FREK is expressed in the rostral and lateral epiblast and in the Hensen's node. From 2.5 days of development (E 2.5) on, it is expressed in various ectoderm- and mesoderm-derived structures. Most striking is FREK expression in the skeletal muscle lineage. It is highly expressed in the early myotome and, at later stages, in all skeletal muscles of the embryo. From E9 to hatching, FREK expression in the muscles decreases dramatically but is maintained in satellite cells of adult muscles. FREK transcript is elevated upon addition of basic fibroblast growth factor to serum-starved satellite cells. From this study, we conclude: (1) that the structure and pattern of expression of FREK set it apart from other cloned fibroblast growth factor receptors (FGFR) and suggest that FREK is a new member of that family; (2) that FREK may play multiple roles in early avian development, including a specialized role in the early differentiation of skeletal muscle.

FGFR-4, a new member of the fibroblast growth factor receptor family, expressed in the definitive endoderm and skeletal muscle lineages of the mouse

Development ◽  
1991 ◽  
Vol 113 (2) ◽  
pp. 641-651 ◽  
Author(s):  
K.L. Stark ◽  
J.A. McMahon ◽  
A.P. McMahon
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Definitive Endoderm ◽  
Developmental Expression ◽  
Fibroblast Growth ◽  
Collecting Tubules ◽  
Receptor Family

We have used the polymerase chain reaction to clone from fetal cerebellar RNA a novel member of the fibroblast growth factor receptor family, FGFR-4. cDNAs encoding a full-length receptor were isolated and RNA expression examined in adult and fetal tissues by RNA blot analysis. Transcripts were detected in adult lung, liver and kidney and in fetal RNAs from 11.5 to 16.5 days post coitum (p.c.). In situ hybridization was performed to examine developmental expression. FGFR-4 RNA was expressed in definitive endoderm of the developing gut and extraembryonic endoderm of the yolk-sac from 8.5 to 14.5 days p.c. At early somite stages, FGFR-4 was also expressed in the myotomal component of the somite, and by 14.5 days p.c. in the myotomally derived skeletal muscle. No expression was seen at any stage in cardiac muscle. Several endodermal derivatives, the liver, lung and pancreas, expressed FGFR-4 at 14.5 days p.c. In addition, FGFR-4 RNA was detected in the adrenal cortex, collecting tubules of the kidney and condensing cartilage at this time. These results suggest that FGFR-4 is likely to have diverse roles in development, which may include regulation of definitive endoderm and skeletal muscle lineages.

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