scholarly journals Not just scratching the surface: distinct radular motion patterns in Mollusca

Biology Open ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. bio055699
Author(s):  
Carolin Scheel ◽  
Stanislav N. Gorb ◽  
Matthias Glaubrecht ◽  
Wencke Krings
Keyword(s):  
Food Processing ◽  
Correct Position ◽  
Sem Images ◽  
Motion Patterns ◽  
Video Footage ◽  
Representative Species ◽  
Different Types ◽  
Functional Tooth ◽  
Movement Type ◽  
Scanning Electron

ABSTRACTThe radula is the organ for mechanical food processing and an important autapomorphy of Mollusca. Its chitinous membrane, embedding small radular teeth, is moved by the set of muscles resulting in an interaction with the ingesta, tearing it and collecting loosened particles. Radulae and their teeth can be quite distinct in their morphology and had been of high research interest, but only a few studies have examined the basic functional principles of this organ, the movement and motion during feeding action. Here, the radular motion of 20 representative species, belonging to four major gastropod lineages (Vetigastropoda, Neritimorpha, Caenogastropoda and Heterobranchia) and Polyplacophora, were recorded and classified. Comparisons of the video footage with the scanning electron microscope (SEM) images of the radula resulted in the recognition of functional tooth rows and the correct position of the teeth during feeding. We identified six different types of radular movements, including rotations and bending of the radula itself. In each movement type, different structures act as counter bearings enabling the animals to grab and tear food.

Treatment on Coconut Shell to Improve its Quality for Use as Coarse Aggregate

2021 ◽  
Vol 1030 ◽  
pp. 94-102
Author(s):  
T. Thilagashanthi ◽  
K. Gunasekaran ◽  
K.S. Satyanarayanan
Keyword(s):  
Coarse Aggregate ◽  
Ferrous Sulphate ◽  
Coconut Shell ◽  
Electron Microscopic ◽  
Sem Images ◽  
Different Types ◽  
Scanning Electron Microscopic ◽  
Imagej Software ◽  
Scanning Electron

To improve the quality of coconut shell (CS) to use as coarse aggregate different treatment methods are given to CS. Polyvinyl alcohol (PVA) and ferrous sulphate (FS) were used as materials for treating the CS. Ten different types of treatments were considered from raw CS to treat with PVA and FS. Significant reduction of water absorbing quality on CS was found from the different types of treatment given. Scanning electron microscopic (SEM) images were also taken and analyzed. SEM images obtained are exported into the software called ImageJ and analyzed in two different ways: one way is using 8 bits and other way is called RGB. An extract from the ImageJ software is given to support the results of treated CS aggregates. If the CS aggregates are treated, then no doubt that the water absorbing capacity of CS can be reduced.

scholarly journals InP nanowires quality control using SEM and Raman spectroscopy

2016 ◽  
Vol 34 (4) ◽  
pp. 851-855
Author(s):  
K. Grodecki ◽  
E. Dumiszewska ◽  
M. Romaniec ◽  
W. Strupinski
Keyword(s):  
Raman Spectroscopy ◽  
Low Energy ◽  
Crystal Quality ◽  
Full Width ◽  
Half Maximum ◽  
Sem Images ◽  
Different Types ◽  
Si Doped ◽  
Scanning Electron

AbstractThree different types of samples of InP nanowires, i.e. undoped, doped with Si and doped with Te, were grown and measured using SEM and Raman spectroscopy. Scanning Electron Microscope (SEM) images showed differences in the length, homogeneity and curvature of the nanowires. The most homogenous wires, grown most perpendicular to the surface, were those Si doped. They were also the shortest. Raman spectroscopy showed that the nanowires doped with Si had the lowest Full Width at Half Maximum (FWHM) TO band, which suggests the highest crystal quality of these wires. For the wires doped with Te, which were the most inhomogeneous, a low energy acoustic band was also observed, which suggests the lowest crystal quality of these structures.

Scanning electron microscopy of asbestos-containing material where the asbestos fibers are considered locked in a binder

1993 ◽  
Vol 51 ◽  
pp. 472-473
Author(s):  
J. R. Millette ◽  
R. S. Brown
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Protection Agency ◽  
Building Materials ◽  
The United States ◽  
Protection Agency ◽  
Asbestos Fibers ◽  
Different Types ◽  
Binder Material ◽  
Scanning Electron

The United States Environmental Protection Agency (EPA) has labeled as “friable” those building materials that are likely to readily release fibers. Friable materials when dry, can easily be crumbled, pulverized, or reduced to powder using hand pressure. Other asbestos containing building materials (ACBM) where the asbestos fibers are in a matrix of cement or bituminous or resinous binders are considered non-friable. However, when subjected to sanding, grinding, cutting or other forms of abrasion, these non-friable materials are to be treated as friable asbestos material. There has been a hypothesis that all raw asbestos fibers are encapsulated in solvents and binders and are not released as individual fibers if the material is cut or abraded. Examination of a number of different types of non-friable materials under the SEM show that after cutting or abrasion, tuffs or bundles of fibers are evident on the surfaces of the materials. When these tuffs or bundles are examined, they are shown to contain asbestos fibers which are free from binder material. These free fibers may be released into the air upon further cutting or abrasion.

scholarly journals Microscopy Methods for Biofilm Imaging: Focus on SEM and VP-SEM Pros and Cons

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
Michela Relucenti ◽  
Giuseppe Familiari ◽  
Orlando Donfrancesco ◽  
Maurizio Taurino ◽  
Xiaobo Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ion Beam ◽  
Ruthenium Red ◽  
Variable Pressure ◽  
Sem Images ◽  
Advantages And Disadvantages ◽  
Pros And Cons ◽  
Microscopy Techniques ◽  
Scanning Electron

Several imaging methodologies have been used in biofilm studies, contributing to deepening the knowledge on their structure. This review illustrates the most widely used microscopy techniques in biofilm investigations, focusing on traditional and innovative scanning electron microscopy techniques such as scanning electron microscopy (SEM), variable pressure SEM (VP-SEM), environmental SEM (ESEM), and the more recent ambiental SEM (ASEM), ending with the cutting edge Cryo-SEM and focused ion beam SEM (FIB SEM), highlighting the pros and cons of several methods with particular emphasis on conventional SEM and VP-SEM. As each technique has its own advantages and disadvantages, the choice of the most appropriate method must be done carefully, based on the specific aim of the study. The evaluation of the drug effects on biofilm requires imaging methods that show the most detailed ultrastructural features of the biofilm. In this kind of research, the use of scanning electron microscopy with customized protocols such as osmium tetroxide (OsO4), ruthenium red (RR), tannic acid (TA) staining, and ionic liquid (IL) treatment is unrivalled for its image quality, magnification, resolution, minimal sample loss, and actual sample structure preservation. The combined use of innovative SEM protocols and 3-D image analysis software will allow for quantitative data from SEM images to be extracted; in this way, data from images of samples that have undergone different antibiofilm treatments can be compared.

scholarly journals Unraveling the fine-tuned lemon coloration of a pierid butterfly Catopsilia pomona

Microscopy ◽  
2017 ◽  
Vol 66 (6) ◽  
pp. 414-423
Author(s):  
Monalisa Mishra ◽  
Ashutosh Choudhury ◽  
P Sagar Achary ◽  
Harekrushna Sahoo
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Variable Temperature ◽  
Wing Pattern ◽  
Physical Composition ◽  
Different Types ◽  
Microscopy Techniques ◽  
Pierid Butterfly ◽  
Best Fit ◽  
Scanning Electron

Abstract Butterflies wings possess different types of scales to perform diverse functions. Each scale has many nano and microstructures, which interferes with light, resulting in unique coloration for each butterfly. Besides coloration, the arrangement of scales further helps in giving better survivability. Thus, analysis of wing pattern provides an overall idea about adaptation and activity of the animal. The current study deciphers the structure and composition of a wing of a pierid butterfly Catopsilia pomona, which remains active at 42°C at which temperature all other butterflies face a tougher task for existence. In order to know the relation between survivability and adaptation in the wing, we have investigated the structural and physical composition of the wing of C. pomona under optical spectroscopy (absorption, reflectance and transmittance) along with microscopy techniques (optical and scanning electron microscopy), which are not described in earlier studies. The current findings reveal unique structural arrangement within scales to provide the best fit to the animal in variable temperature.

The Photoluminescence Enhancement and Stability of Porous Silicon by Cathodic Reduction and Acid Treatment

2014 ◽  
Vol 887-888 ◽  
pp. 458-461
Author(s):  
Chang Qing Li ◽  
Kun Wang ◽  
Pei Jia Liu ◽  
Qi Ming
Keyword(s):  
Electron Microscope ◽  
Porous Silicon ◽  
Acid Treatment ◽  
Cathodic Reduction ◽  
Luminescence Properties ◽  
Sem Images ◽  
Photoluminescence Enhancement ◽  
The Stability ◽  
Scanning Electron ◽  
Cathode Reduction

Porous silicon (PSi) was fabricated by using electrochemical anodic etching method. Then acid treatment and cathode reduction treatment were employed to improve the luminescence properties and stability of PSi material. Photoluminescence (PL) measurements and scanning electron microscope (SEM) were used to observe the luminescence properties and microstructure of samples, respectively. The results of PL measurements showed that the PL intensity and the stability of luminescence of samples after cathodic reduction and acid treatment were significantly improved. The SEM images showed that the porosity of PSi may be increased through the cathodic reduction treated.

scholarly journals Possible source of ancient carbon in phytolith concentrates from harvested grasses

2012 ◽  
Vol 9 (5) ◽  
pp. 1873-1884 ◽  
Author(s):  
G. M. Santos ◽  
A. Alexandre ◽  
J. R. Southon ◽  
K. K. Treseder ◽  
R. Corbineau ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Atmospheric Carbon Dioxide ◽  
Energy Dispersive Spectrometer ◽  
Nutrient Acquisition ◽  
Published Data ◽  
Plant Nutrient ◽  
Before Present ◽  
Sem Images ◽  
Carbon Dioxide Co2 ◽  
Scanning Electron

Abstract. Plants absorb and transport silicon (Si) from soil, and precipitation of Si within the living plants results in micrometric amorphous biosilica particles known as phytoliths. During phytolith formation, a small amount of carbon (<2%) can become occluded in the silica structure (phytC) and therefore protected from degradation by the environment after plant tissue decomposition. Since the major C source within plants is from atmospheric carbon dioxide (CO2) via photosynthesis, the current understanding is that the radiocarbon (14C) content of phytC should reflect the 14C content of atmospheric CO2 at the time the plant is growing. This assumption was recently challenged by 14C data from phytoliths extracted from living grasses that yielded ages of several thousand years (2–8 kyr BP; in radiocarbon years "Before Present" (BP), "Present" being defined as 1950). Because plants can take up small amounts of C of varying ages from soils (e.g., during nutrient acquisition), we hypothesized that this transported C within the plant tissue could be attached to or even embedded in phytoliths. In this work, we explore this hypothesis by reviewing previously published data on biosilica mineralization and plant nutrient acquisition as well as by evaluating the efficiency of phytolith extraction protocols from scanning electron microscope (SEM) images and energy dispersive spectrometer (EDS) analyses from harvested grasses phytolith concentrates. We show that current extraction protocols are inefficient since they do not entirely remove recalcitrant forms of C from plant tissue. Consequently, material previously measured as "phytC" may contain at least some fraction of soil-derived C (likely radiocarbon-old) taken up by roots. We also suggest a novel interpretation for at least some of the phytC – which enters via the root pathway during nutrient acquisition – that may help to explain the old ages previously obtained from phytolith concentrates.

scholarly journals Apolar Bioactive Fraction ofMelipona scutellarisGeopropolis onStreptococcus mutansBiofilm

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Marcos Guilherme da Cunha ◽  
Marcelo Franchin ◽  
Lívia Câmara de Carvalho Galvão ◽  
Bruno Bueno-Silva ◽  
Masaharu Ikegaki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Biofilm Formation ◽  
Microbial Population ◽  
Ethanolic Extract ◽  
Vehicle Control ◽  
Sem Images ◽  
Killing Assay ◽  
Bioactive Fraction ◽  
Biofilm Matrix ◽  
Scanning Electron

The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis onStreptococcus mutansbiofilm. The ethanolic extract ofMelipona scutellarisgeopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF onS. mutansUA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 μg/mL and 400 μg/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P<0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins. In addition, theS. mutansviability (killing assay) and acid production by glycolytic pH drop were not affected (P>0.05). In conclusion, the bioactive HF of geopropolis was promising to control theS. mutansbiofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.

Hierarchical Design and Nanomechanics of the Calcified Byssus of Anomia simplex

2009 ◽  
Vol 1187 ◽  
Author(s):  
Jakob R Eltzholtz ◽  
Marie Krogsgaard ◽  
Henrik Birkedal
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Mechanical Performance ◽  
Blue Mussel ◽  
Hierarchical Design ◽  
Reduced Modulus ◽  
Different Types ◽  
Microscopy Images ◽  
Scanning Electron

AbstractBiology has evolved several strategies for attachment of sedentary animals. In the bivalves, byssi abound and the best known example being the protein-based byssus of the blue mussel and other Mytilidae. In contrast the bivalve Anomia sp. has a single calcified thread. The byssus is hierarchical in design and contains several different types of structures as revealed by scanning electron microscopy images. The mechanical properties of the byssus are probed by nanoindentation. It is found that the mineralized part of the byssus is very stiff with a reduced modulus of about 67 GPa and a hardness of ˜3.7 GPa. This corresponds to a modulus roughly 20% smaller than that of pure calcite and a hardness that is about 20% larger than pure calcite. The results reveal the importance of microstructure on mechanical performance.

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