scholarly journals Prostaglandin E2 promotes embryonic vascular development and maturation in zebrafish

Biology Open ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. bio039768 ◽  
Author(s):  
Kingsley Chukwunonso Ugwuagbo ◽  
Sujit Maiti ◽  
Ahmed Omar ◽  
Stephanie Hunter ◽  
Braydon Nault ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Vascular Development ◽  
Embryonic Vascular Development

scholarly journals Tyrosine 1101 of Tie2 Is the Major Site of Association of p85 and Is Required for Activation of Phosphatidylinositol 3-Kinase and Akt

1998 ◽  
Vol 18 (7) ◽  
pp. 4131-4140 ◽  
Author(s):  
Christopher D. Kontos ◽  
Thomas P. Stauffer ◽  
Wen-Pin Yang ◽  
John D. York ◽  
Liwen Huang ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Vascular Development ◽  
Pi3 Kinase ◽  
Pleckstrin Homology Domain ◽  
Phosphatidylinositol 3 Kinase ◽  
Lipid Kinase ◽  
Embryonic Vascular Development ◽  
Phosphatidylinositol 3

ABSTRACT Tie2 is an endothelium-specific receptor tyrosine kinase that is required for both normal embryonic vascular development and tumor angiogenesis and is thought to play a role in vascular maintenance. However, the signaling pathways responsible for the function of Tie2 remain unknown. In this report, we demonstrate that the p85 subunit of phosphatidylinositol 3-kinase (PI3-kinase) associates with Tie2 and that this association confers functional lipid kinase activity. Mutation of tyrosine 1101 of Tie2 abrogated p85 association both in vitro and in vivo in yeast. Tie2 was found to activate PI3-kinase in vivo as demonstrated by direct measurement of increases in cellular phosphatidylinositol 3-phosphate and phosphatidylinositol 3,4-bisphosphate, by plasma membrane translocation of a green fluorescent protein-Akt pleckstrin homology domain fusion protein, and by downstream activation of the Akt kinase. Activation of PI3-kinase was abrogated in these assays by mutation of Y1101 to phenylalanine, consistent with a requirement for this residue for p85 association with Tie2. These results suggest that activation of PI3-kinase and Akt may in part account for Tie2’s role in both embryonic vascular development and pathologic angiogenesis, and they are consistent with a role for Tie2 in endothelial cell survival.

scholarly journals SRG3, a core component of mouse SWI/SNF complex, is essential for extra-embryonic vascular development

2008 ◽  
Vol 315 (1) ◽  
pp. 136-146 ◽  
Author(s):  
Daehee Han ◽  
Shin Jeon ◽  
Dong Hyun Sohn ◽  
Changjin Lee ◽  
Sangil Ahn ◽  
...  
Keyword(s):  
Vascular Development ◽  
Core Component ◽  
Embryonic Vascular Development

Notch signaling is required for arterial-venous differentiation during embryonic vascular development

Development ◽  
2001 ◽  
Vol 128 (19) ◽  
pp. 3675-3683 ◽  
Author(s):  
Nathan D. Lawson ◽  
Nico Scheer ◽  
Van N. Pham ◽  
Cheol-Hee Kim ◽  
Ajay B. Chitnis ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Blood Vessels ◽  
Cell Fate ◽  
Venous Blood ◽  
Vascular Development ◽  
Ectopic Expression ◽  
Specific Expression ◽  
Ectopic Activation ◽  
On Line ◽  
Embryonic Vascular Development

Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression of a number of Notch signaling genes in mouse and zebrafish suggests that this pathway may play a role in arterial-venous cell fate determination during vascular development. We show that loss of Notch signaling in zebrafish embryos leads to molecular defects in arterial-venous differentiation, including loss of artery-specific markers and ectopic expression of venous markers within the dorsal aorta. Conversely, we find that ectopic activation of Notch signaling leads to repression of venous cell fate. Finally, embryos lacking Notch function exhibit defects in blood vessel formation similar to those associated with improper arterial-venous specification. Our results suggest that Notch signaling is required for the proper development of arterial and venous blood vessels, and that a major role of Notch signaling in blood vessels is to repress venous differentiation within developing arteries.Movies available on-line

scholarly journals Time-lapse microscopy of macrophages during embryonic vascular development

2012 ◽  
Vol 241 (9) ◽  
pp. 1423-1431 ◽  
Author(s):  
Sarah Al-Roubaie ◽  
Jasmine H. Hughes ◽  
Michael B. Filla ◽  
Rusty Lansford ◽  
Stephanie Lehoux ◽  
...  
Keyword(s):  
Vascular Development ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Embryonic Vascular Development
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