scholarly journals In Vivo Imaging of Embryonic Vascular Development Using Transgenic Zebrafish

2002 ◽  
Vol 248 (2) ◽  
pp. 307-318 ◽  
Author(s):  
Nathan D. Lawson ◽  
Brant M. Weinstein
Keyword(s):  
In Vivo Imaging ◽  
Vascular Development ◽  
Transgenic Zebrafish ◽  
Embryonic Vascular Development

scholarly journals Tyrosine 1101 of Tie2 Is the Major Site of Association of p85 and Is Required for Activation of Phosphatidylinositol 3-Kinase and Akt

1998 ◽  
Vol 18 (7) ◽  
pp. 4131-4140 ◽  
Author(s):  
Christopher D. Kontos ◽  
Thomas P. Stauffer ◽  
Wen-Pin Yang ◽  
John D. York ◽  
Liwen Huang ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Vascular Development ◽  
Pi3 Kinase ◽  
Pleckstrin Homology Domain ◽  
Phosphatidylinositol 3 Kinase ◽  
Lipid Kinase ◽  
Embryonic Vascular Development ◽  
Phosphatidylinositol 3

ABSTRACT Tie2 is an endothelium-specific receptor tyrosine kinase that is required for both normal embryonic vascular development and tumor angiogenesis and is thought to play a role in vascular maintenance. However, the signaling pathways responsible for the function of Tie2 remain unknown. In this report, we demonstrate that the p85 subunit of phosphatidylinositol 3-kinase (PI3-kinase) associates with Tie2 and that this association confers functional lipid kinase activity. Mutation of tyrosine 1101 of Tie2 abrogated p85 association both in vitro and in vivo in yeast. Tie2 was found to activate PI3-kinase in vivo as demonstrated by direct measurement of increases in cellular phosphatidylinositol 3-phosphate and phosphatidylinositol 3,4-bisphosphate, by plasma membrane translocation of a green fluorescent protein-Akt pleckstrin homology domain fusion protein, and by downstream activation of the Akt kinase. Activation of PI3-kinase was abrogated in these assays by mutation of Y1101 to phenylalanine, consistent with a requirement for this residue for p85 association with Tie2. These results suggest that activation of PI3-kinase and Akt may in part account for Tie2’s role in both embryonic vascular development and pathologic angiogenesis, and they are consistent with a role for Tie2 in endothelial cell survival.

scholarly journals Zebrafish as a Model for Fish Diseases in Aquaculture

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 609
Author(s):  
Louise von Gersdorff Jørgensen
Keyword(s):  
Animal Models ◽  
Fish Species ◽  
In Vivo Imaging ◽  
Transgenic Zebrafish ◽  
Parasitic Diseases ◽  
Fish Diseases ◽  
Immunological Responses ◽  
Infection Biology

The use of zebrafish as a model for human conditions is widely recognized. Within the last couple of decades, the zebrafish has furthermore increasingly been utilized as a model for diseases in aquacultured fish species. The unique tools available in zebrafish present advantages compared to other animal models and unprecedented in vivo imaging and the use of transgenic zebrafish lines have contributed with novel knowledge to this field. In this review, investigations conducted in zebrafish on economically important diseases in aquacultured fish species are included. Studies are summarized on bacterial, viral and parasitic diseases and described in relation to prophylactic approaches, immunology and infection biology. Considerable attention has been assigned to innate and adaptive immunological responses. Finally, advantages and drawbacks of using the zebrafish as a model for aquacultured fish species are discussed.

In vivo imaging of neural activities along the olfactory circuitry in transgenic zebrafish

2011 ◽  
Vol 71 ◽  
pp. e357
Author(s):  
Tetsuya Koide ◽  
Masamichi Ohkura ◽  
Junichi Nakai ◽  
Yoshihiro Yoshihara
Keyword(s):  
In Vivo Imaging ◽  
Transgenic Zebrafish ◽  
Neural Activities

Generation and characterisation of novel transgenic zebrafish allowing in vivo imaging of endothelial cell biology

2016 ◽  
Vol 244 ◽  
pp. e10 ◽  
Author(s):  
A.M. Savage ◽  
C. Mayo ◽  
H.R. Kim ◽  
E. Markham ◽  
F.J.M. van Eeden ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
In Vivo Imaging ◽  
Cell Biology ◽  
Transgenic Zebrafish

scholarly journals Development of Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper(roy−/−,nacre−/−) Transparent Transgenic In Vivo Zebrafish Model to Study the Cardiomyocyte Function

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1963
Author(s):  
Surendra K. Rajpurohit ◽  
Aaron Gopal ◽  
May Ye Mon ◽  
Nikhil G. Patel ◽  
Vishal Arora
Keyword(s):  
In Vivo Imaging ◽  
High Throughput Screening ◽  
Skin Pigmentation ◽  
Fluorescent Microscopy ◽  
Confocal Imaging ◽  
Transgenic Zebrafish ◽  
Cellular Phenotype ◽  
Zebrafish Model ◽  
Over Time

The zebrafish provided an excellent platform to study the genetic and molecular approach of cellular phenotype-based cardiac research. We designed a novel protocol to develop the transparent transgenic zebrafish model to study annexin-5 activity in the cardiovascular function by generating homozygous transparent skin Casper (roy−/−,nacre−/−); myl7:RFP; annexin-5:YFP transgenic zebrafish. The skin pigmentation background of any vertebrate model organism is a major obstruction for in vivo confocal imaging to study the transgenic cellular phenotype-based study. By developing Casper (roy−/−,nacre−/−); myl7; annexin-5 transparent transgenic zebrafish strain, we established time-lapse in vivo confocal microscopy to study cellular phenotype/pathologies of cardiomyocytes over time to quantify changes in cardiomyocyte morphology and function over time, comparing control and cardiac injury and cardio-oncology. Casper contributes to the study by integrating a transparent characteristic in adult zebrafish that allows for simpler transparent visualization and observation. The Casper (roy−/−,nacre−/−) transgenic progenies developed through cross-breeding with the transgenic strain of Tg (UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP). Confocal and fluorescent microscopy were being used to obtain accurate, precise imaging and to determine fluorescent protein being activated. This study protocol was conducted under two sections; 1.1: Generation of homozygous Tg (UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper (roy−/−,nacre−/−) zebrafish (generation F01-F06) and 1.2: Screening and sorting the transparent transgenic progeny and in vivo imaging to validate cardiac morphology through in vivo confocal imaging. We coined the newly developed strain as Tg (UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper (roy−/−,nacre−/−) gmc1. Thus, the newly developed strain maintains transparency of the skin throughout the entire life of zebrafish and is capable of application of a non-invasive in vivo imaging process. These novel results provide an in vivo whole organism-based platform to design high-throughput screening and establish a new horizon for drug discovery in cardiac cell death and cardio-oncology therapeutics and treatment.

scholarly journals Platelets regulate lymphatic vascular development through CLEC-2–SLP-76 signaling

Blood ◽  
2010 ◽  
Vol 116 (4) ◽  
pp. 661-670 ◽  
Author(s):  
Cara C. Bertozzi ◽  
Alec A. Schmaier ◽  
Patricia Mericko ◽  
Paul R. Hess ◽  
Zhiying Zou ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ex Vivo ◽  
Transmembrane Protein ◽  
Vascular Development ◽  
Lymphatic Endothelial Cells ◽  
Cell Type ◽  
Platelet Factor ◽  
Platelet Aggregate ◽  
Embryonic Vascular Development

Abstract Although platelets appear by embryonic day 10.5 in the developing mouse, an embryonic role for these cells has not been identified. The SYK–SLP-76 signaling pathway is required in blood cells to regulate embryonic blood-lymphatic vascular separation, but the cell type and molecular mechanism underlying this regulatory pathway are not known. In the present study we demonstrate that platelets regulate lymphatic vascular development by directly interacting with lymphatic endothelial cells through C-type lectin-like receptor 2 (CLEC-2) receptors. PODOPLANIN (PDPN), a transmembrane protein expressed on the surface of lymphatic endothelial cells, is required in nonhematopoietic cells for blood-lymphatic separation. Genetic loss of the PDPN receptor CLEC-2 ablates PDPN binding by platelets and confers embryonic lymphatic vascular defects like those seen in animals lacking PDPN or SLP-76. Platelet factor 4-Cre–mediated deletion of Slp-76 is sufficient to confer lymphatic vascular defects, identifying platelets as the cell type in which SLP-76 signaling is required to regulate lymphatic vascular development. Consistent with these genetic findings, we observe SLP-76–dependent platelet aggregate formation on the surface of lymphatic endothelial cells in vivo and ex vivo. These studies identify a nonhemostatic pathway in which platelet CLEC-2 receptors bind lymphatic endothelial PDPN and activate SLP-76 signaling to regulate embryonic vascular development.

scholarly journals Generation of a transgenic zebrafish line suitable for in vivo imaging of apoptosis in liver

2021 ◽  
Vol 94 (0) ◽  
pp. 1-O-G1-1
Author(s):  
Aina Higuchi ◽  
Eri Wakai ◽  
Yuka Adachi ◽  
Tomoko Tada ◽  
Junko Koiwa ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Transgenic Zebrafish
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